Restriction map for
pF1KE6626
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII ClaI
CpoI DpnII DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeIII MamI
MboI MfeI MflI MluI MroI
MseI MslI MunI NcoI NdeI
NdeII NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I Tth111I XbaI XcmI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AlwNI 748 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 163 CCTGAGG CC^T[ACGT]AGG
ApaI 151 GGGCCC GGGCC^C
AspHI 685 GTGCTC G[AT]GC[AT]^C
AvaI 442 CTCGAG C^[CT]CG[GA]G
BalI 836 TGGCCA TGG^CCA
BbsI 694 GAAGAC GAAGAC(2/6)
BbvCI 760 GCTGAGG CCTCAGC(-5/-2)
BglI 829 GCCGCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 694 GAAGAC GAAGAC(2/6)
BsaI 613 GAGACC GGTCTC(1/5)
BsgI 106 CTGCAC GTGCAG(16/14)
BsiEI 789 CGACCG CG[GA][CT]^CG
BsiHKAI 685 GTGCTC G[AT]GC[AT]^C
BsmAI 613 GAGAC GTCTC(1/5)
BsoBI 442 CTCGAG C^[CT]CG[GA]G
BspMI 752 ACCTGC ACCTGC(4/8)
BsrFI 91 GCCGGC [GA]^CCGG[CT]
BsrI 825 ACTGG ACTGG(1/-1)
Bsu36I 163 CCTGAGG CC^T[ACGT]AGG
Cfr10I 91 GCCGGC [GA]^CCGG[CT]
DsaI 833 CCGTGG C^C[GA][CT]GG
EarI 55 CTCTTC CTCTTC(1/4)
Eco81I 163 CCTGAGG CC^T[ACGT]AGG
EcoT14I 677 CCAAGG C^C[AT][AT]GG
HgaI 231 GCGTC GACGC(5/10)
Ksp632I 55 CTCTTC CTCTTC(1/4)
MaeII 423 ACGT A^CGT
MluNI 836 TGGCCA TGG^CCA
MscI 836 TGGCCA TGG^CCA
NaeI 91 GCCGGC GCC^GGC
NgoMIV 91 GCCGGC G^CCGGC
PaeR7I 442 CTCGAG C^TCGAG
PflMI 240 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 440 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI 220 CTACAG C^T[GA][CT]AG
SfiI 828 GGCCGCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 442 CTCGAG C^T[CT][GA]AG
StyI 677 CCAAGG C^C[AT][AT]GG
Tsp509I 348 AATT ^AATT
TspRI 104 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 240 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 442 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 6 GGCGCC G[GA]^CG[CT]C
194 GGCGCC
AfaI 18 GTAC GT^AC
564 GTAC
AvaII 204 GGACC G^G[AT]CC
750 GGACC
BanI 6 GGCGCC G^G[CT][GA]CC
194 GGCGCC
BanII 151 GGGCCC G[GA]GC[CT]^C
236 GAGCCC
BbeI 6 GGCGCC GGCGC^C
194 GGCGCC
BbvI 68 GCAGC GCAGC(8/12)
212 GCAGC
BfaI 40 CTAG C^TAG
292 CTAG
BsaHI 6 GGCGCC G[GA]^CG[CT]C
194 GGCGCC
BsrBI 430 GAGCGG CCGCTC(-3/-3)
481 GAGCGG
EcoNI 291 CCTAGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
492 CCTGGAGGAGG
HaeII 6 GGCGCC [GA]GCGC^[CT]
194 GGCGCC
Hin1I 6 GGCGCC G[GA]^CG[CT]C
194 GGCGCC
HphI 333 TCACC GGTGA(8/7)
905 TCACC
KasI 6 GGCGCC G^GCGCC
194 GGCGCC
MaeI 40 CTAG C^TAG
292 CTAG
NarI 6 GGCGCC GG^CGCC
194 GGCGCC
NlaIII 556 CATG CATG^
618 CATG
PpuMI 203 GGGACCT [GA]G^G[AT]CC[CT]
749 AGGACCT
RsaI 18 GTAC GT^AC
564 GTAC
SfaNI 85 GCATC GCATC(5/9)
743 GCATC
SfoI 6 GGCGCC GGC^GCC
194 GGCGCC
StuI 187 AGGCCT AGG^CCT
581 AGGCCT
TseI 68 GCAGC G^C[AT]GC
212 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AluI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV PleI Sau96I
ScrFI TaqI TthHB8I