Restriction map for
pF1KE2534
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BanI
BbeI BbrPI BbsI BciVI BfaI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MseI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 418 GTATAC GT^[AC][GT]AC
AocI 505 CCTGAGG CC^T[ACGT]AGG
ApaI 274 GGGCCC GGGCC^C
AspI 679 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 11 CCTCAGC CCTCAGC(-5/-2)
BcgI 1001 CGATTGGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 944 TGATCA T^GATCA
BglI 1015 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 381 AGATCT A^GATCT
BpmI 447 CTCCAG CTGGAG(16/14)
BsaWI 403 ACCGGA [AT]^CCGG[AT]
BsgI 797 CTGCAC GTGCAG(16/14)
Bst1107I 418 GTATAC GTA^TAC
BstXI 461 CCAACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 418 GTATAC GTA^TAC
Bsu36I 505 CCTGAGG CC^T[ACGT]AGG
DsaI 467 CCGTGG C^C[GA][CT]GG
Eco81I 505 CCTGAGG CC^T[ACGT]AGG
EcoT22I 311 ATGCAT ATGCA^T
FauI 277 CCCGC CCCGC(4/6)
FbaI 944 TGATCA T^GATCA
HgaI 611 GCGTC GACGC(5/10)
MslI 864 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 311 ATGCAT ATGCA^T
NspI 33 GCATGT [GA]CATG^[CT]
PflFI 679 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 930 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 847 GAGCTC GAGCT^C
ScaI 803 AGTACT AGT^ACT
TaqI 291 TCGA T^CGA
Tth111I 679 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 291 TCGA T^CGA
XcmI 177 CCAGAATCGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 201 GTAC GT^AC
804 GTAC
AlwNI 911 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
950 CAGTCTCTG
AspHI 847 GAGCTC G[AT]GC[AT]^C
962 GTGCTC
BalI 983 TGGCCA TGG^CCA
1013 TGGCCA
BamHI 631 GGATCC G^GATCC
883 GGATCC
BanII 274 GGGCCC G[GA]GC[CT]^C
847 GAGCTC
BcnI 423 CCCGG CC^[GC]GG
555 CCCGG
BsiHKAI 847 GAGCTC G[AT]GC[AT]^C
962 GTGCTC
BsmAI 295 GAGAC GTCTC(1/5)
952 GTCTC
BspMI 170 ACCTGC ACCTGC(4/8)
795 ACCTGC
BsrI 916 ACTGG ACTGG(1/-1)
682 CCAGT
Cac8I 276 GCCCGC GC[ACGT]^[ACGT]GC
1011 GCTGGC
EaeI 983 TGGCCA [CT]^GGCC[GA]
1013 TGGCCA
MaeII 332 ACGT A^CGT
826 ACGT
MboII 394 GAAGA GAAGA(8/7)
153 TCTTC
MluNI 983 TGGCCA TGG^CCA
1013 TGGCCA
MscI 983 TGGCCA TGG^CCA
1013 TGGCCA
MwoI 429 GCTGCCCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1015 GCCAGGGTGGC
NciI 423 CCCGG CC^[GC]GG
555 CCCGG
PpuMI 3 GGGACCC [GA]G^G[AT]CC[CT]
50 AGGTCCT
PstI 24 CTGCAG CTGCA^G
571 CTGCAG
RsaI 201 GTAC GT^AC
804 GTAC
SfcI 24 CTGCAG C^T[GA][CT]AG
571 CTGCAG
Tsp509I 234 AATT ^AATT
742 AATT
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MflI MnlI MspA1I MspI
MvaI NdeII NlaIII NlaIV PvuII
Sau3AI Sau96I ScrFI SfaNI TfiI
TseI Tsp45I TspRI XhoII