Restriction map for
pF1KE1761
##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI BalI
BamHI BbeI BbrPI BbvCI BciVI
BfrI BglII BlpI Bpu1102I BsaAI
BsaBI BsaI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FseI HindIII KasI KpnI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MluNI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccIII 302 TCCGGA T^CCGGA
AcsI 487 GAATTC [GA]^AATT[CT]
AcyI 709 GACGCC G[GA]^CG[CT]C
AflIII 427 ACATGT A^C[GA][CT]GT
ApoI 487 GAATTC [GA]^AATT[CT]
AvrII 183 CCTAGG C^CTAGG
BanI 586 GGTGCC G^G[CT][GA]CC
BanII 235 GAGCCC G[GA]GC[CT]^C
BbsI 402 GAAGAC GAAGAC(2/6)
BcgI 210 CGAGGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 184 CTAG C^TAG
BlnI 183 CCTAGG C^CTAGG
BmyI 235 GAGCCC G[AGT]GC[ACT]^C
BpmI 326 CTGGAG CTGGAG(16/14)
BpuAI 402 GAAGAC GAAGAC(2/6)
BsaHI 709 GACGCC G[GA]^CG[CT]C
BsaWI 302 TCCGGA [AT]^CCGG[AT]
BseAI 302 TCCGGA T^CCGGA
BseRI 286 GAGGAG GAGGAG(10/8)
BsiYI 60 CCTGGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 60 CCTGGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 306 GAGAC GTCTC(1/5)
BsmFI 559 GGGAC GGGAC(10/14)
Bsp1286I 235 GAGCCC G[AGT]GC[ACT]^C
BspEI 302 TCCGGA T^CCGGA
BspHI 359 TCATGA T^CATGA
BspLU11I 427 ACATGT A^CATGT
BsrI 751 ACTGG ACTGG(1/-1)
BstXI 318 CCAGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 651 CTCAG C^T[ACGT]AG
DrdI 334 GACATGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 487 GAATTC G^AATTC
EcoT14I 183 CCTAGG C^C[AT][AT]GG
Hin1I 709 GACGCC G[GA]^CG[CT]C
HincII 10 GTTAAC GT[CT]^[GA]AC
HindII 10 GTTAAC GT[CT]^[GA]AC
HpaI 10 GTTAAC GTT^AAC
HphI 765 GGTGA GGTGA(8/7)
MaeI 184 CTAG C^TAG
MaeIII 382 GTGAC ^GT[ACGT]AC
MboII 402 GAAGA GAAGA(8/7)
MroI 302 TCCGGA T^CCGGA
MseI 11 TTAA T^TAA
MslI 610 CACCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 427 ACATGT [GA]CATG^[CT]
PshAI 424 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 784 CTGCAG CTGCA^G
RcaI 359 TCATGA T^CATGA
ScaI 494 AGTACT AGT^ACT
SfcI 784 CTGCAG C^T[GA][CT]AG
StuI 90 AGGCCT AGG^CCT
StyI 183 CCTAGG C^C[AT][AT]GG
TaqI 542 TCGA T^CGA
Tru9I 11 TTAA T^TAA
Tsp45I 382 GTGAC ^GT[GC]AC
Tsp509I 488 AATT ^AATT
TthHB8I 542 TCGA T^CGA
XcmI 464 CCACAGGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 225 GTAC GT^AC
495 GTAC
AvaII 291 GGTCC G^G[AT]CC
560 GGACC
AviII 176 TGCGCA TGC^GCA
219 TGCGCA
BclI 95 TGATCA T^GATCA
635 TGATCA
BcnI 107 CCGGG CC^[GC]GG
231 CCCGG
BglI 316 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
589 GCCTTTGAGGC
DraII 56 GGGGCCT [GA]G^G[ACGT]CC[CT]
68 GGGGCCT
EaeI 170 CGGCCA [CT]^GGCC[GA]
443 TGGCCG
EcoO109I 56 GGGGCCT [GA]G^G[ACGT]CC[CT]
68 GGGGCCT
FbaI 95 TGATCA T^GATCA
635 TGATCA
FspI 176 TGCGCA TGC^GCA
219 TGCGCA
HaeII 199 AGCGCC [GA]GCGC^[CT]
346 AGCGCC
HgaI 644 GACGC GACGC(5/10)
709 GACGC
HinfI 191 GAGTC G^A[ACGT]TC
308 GACTC
NciI 107 CCGGG CC^[GC]GG
231 CCCGG
PleI 308 GACTC GAGTC(4/5)
191 GAGTC
RsaI 225 GTAC GT^AC
495 GTAC
SfaNI 179 GCATC GCATC(5/9)
621 GCATC
TspRI 546 CACTG CA[GC]TG[ACGT][ACGT]^
750 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI BbvI BsaJI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DpnII EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MboI MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PvuII Sau3AI Sau96I ScrFI TseI