Restriction map for
pF1KE2510
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# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AcsI AflII AflIII AhdI
Aor51HI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbrPI
BciVI BfrI BlnI BsaAI BsaBI
BsaI BsiWI BspDI BspHI BspLU11I
BsrDI BssHII BstBI ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoRI
EcoRV FspI HindIII HpaI KpnI
MamI MfeI MluI MseI MunI
NdeI NheI NruI NspV PacI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI RsrII SalI SapI
ScaI SexAI SfiI SfuI SnaBI
SpeI SspI StuI SwaI Tru9I
Tsp509I XbaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 1519 GACGTC GACGT^C
AccI 588 GTATAC GT^[AC][GT]AC
AclI 166 AACGTT AA^CGTT
AgeI 332 ACCGGT A^CCGGT
Alw44I 1273 GTGCAC G^TGCAC
AocI 2354 CCTCAGG CC^T[ACGT]AGG
ApaLI 1273 GTGCAC G^TGCAC
BamHI 1106 GGATCC G^GATCC
BclI 578 TGATCA T^GATCA
BglII 1894 AGATCT A^GATCT
BsmBI 815 CGTCTC CGTCTC(1/5)
BsmI 1129 GCATTC GAATGC(1/-1)
Bsp1407I 3275 TGTACA T^GTACA
BsrGI 3275 TGTACA T^GTACA
Bst1107I 588 GTATAC GTA^TAC
BstAPI 897 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 258 GGTGACC G^GT[ACGT]ACC
BstPI 258 GGTGACC G^GT[ACGT]ACC
BstZ17I 588 GTATAC GTA^TAC
Bsu36I 2354 CCTCAGG CC^T[ACGT]AGG
Eco81I 2354 CCTCAGG CC^T[ACGT]AGG
EcoO65I 258 GGTGACC G^GT[ACGT]ACC
EcoT22I 1375 ATGCAT ATGCA^T
FbaI 578 TGATCA T^GATCA
FseI 855 GGCCGGCC GGCCGG^CC
KspI 130 CCGCGG CCGC^GG
NotI 565 GCGGCCGC GC^GGCCGC
NsiI 1375 ATGCAT ATGCA^T
PaeR7I 116 CTCGAG C^TCGAG
PinAI 332 ACCGGT A^CCGGT
Psp1406I 166 AACGTT AA^CGTT
SacII 130 CCGCGG CCGC^GG
SgrAI 331 CACCGGTG C[GA]^CCGG[CT]G
SspBI 3275 TGTACA T^GTACA
TfiI 917 GAATC G^A[AT]TC
XhoI 116 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccII 131 CGCG CG^CG
1455 CGCG
AccIII 290 TCCGGA T^CCGGA
366 TCCGGA
Asp700I 1773 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3098 GAAGCCGTTC
BbeI 600 GGCGCC GGCGC^C
2996 GGCGCC
BbsI 1773 GAAGAC GAAGAC(2/6)
1062 GTCTTC
BpuAI 1773 GAAGAC GAAGAC(2/6)
1062 GTCTTC
BseAI 290 TCCGGA T^CCGGA
366 TCCGGA
BspEI 290 TCCGGA T^CCGGA
366 TCCGGA
BssSI 1087 CACGAG CACGAG(-5/-1)
842 CTCGTG
BstUI 131 CGCG CG^CG
1455 CGCG
BstXI 1209 CCAGCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1424 CCATGGAGCTGG
DraIII 1024 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1147 CACAGCGTG
HaeII 600 GGCGCC [GA]GCGC^[CT]
2996 GGCGCC
HincII 169 GTTGAC GT[CT]^[GA]AC
3025 GTCAAC
HindII 169 GTTGAC GT[CT]^[GA]AC
3025 GTCAAC
KasI 600 GGCGCC G^GCGCC
2996 GGCGCC
MroI 290 TCCGGA T^CCGGA
366 TCCGGA
MvnI 131 CGCG CG^CG
1455 CGCG
NarI 600 GGCGCC GG^CGCC
2996 GGCGCC
NcoI 1424 CCATGG C^CATGG
2471 CCATGG
SacI 2727 GAGCTC GAGCT^C
3256 GAGCTC
SbfI 1134 CCTGCAGG CCTGCA^GG
1561 CCTGCAGG
SfoI 600 GGCGCC GGC^GCC
2996 GGCGCC
SmaI 2391 CCCGGG CCC^GGG
2406 CCCGGG
SmlI 116 CTCGAG C^T[CT][GA]AG
2481 CTCAAG
Sse8387I 1134 CCTGCAGG CCTGCA^GG
1561 CCTGCAGG
XmaCI 2391 CCCGGG C^CCGGG
2406 CCCGGG
XmaI 2391 CCCGGG C^CCGGG
2406 CCCGGG
XmnI 1773 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3098 GAAGCCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AspI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI StyI TaqI TseI Tsp45I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII