Restriction map for
pF1KE2333
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BbsI
BbvCI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CelII CfoI ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
MaeI MamI MfeI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1044 GACGTC GACGT^C
AcsI 1083 GAATTT [GA]^AATT[CT]
AfaI 1332 GTAC GT^AC
AflIII 1244 ACGCGT A^C[GA][CT]GT
Alw44I 684 GTGCAC G^TGCAC
AlwNI 374 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 562 CCTCAGG CC^T[ACGT]AGG
ApaI 13 GGGCCC GGGCC^C
ApaLI 684 GTGCAC G^TGCAC
ApoI 1083 GAATTT [GA]^AATT[CT]
BamHI 916 GGATCC G^GATCC
BanI 402 GGTGCC G^G[CT][GA]CC
BcgI 287 GCAACAGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsgI 1265 GTGCAG GTGCAG(16/14)
BspMI 252 GCAGGT ACCTGC(4/8)
BsrFI 925 ACCGGC [GA]^CCGG[CT]
BssSI 331 CACGAG CACGAG(-5/-1)
BstYI 916 GGATCC [GA]^GATC[CT]
Bsu36I 562 CCTCAGG CC^T[ACGT]AGG
Cfr10I 925 ACCGGC [GA]^CCGG[CT]
DraI 1087 TTTAAA TTT^AAA
Eco81I 562 CCTCAGG CC^T[ACGT]AGG
MflI 916 GGATCC [GA]^GATC[CT]
MluI 1244 ACGCGT A^CGCGT
MslI 412 CACAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 441 CCATGG C^CATGG
NspI 1302 GCATGC [GA]CATG^[CT]
PpuMI 1136 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 185 CAGCTG CAG^CTG
RsaI 1332 GTAC GT^AC
SacI 569 GAGCTC GAGCT^C
ScaI 1331 AGTACT AGT^ACT
SexAI 1133 ACCAGGT A^CC[AT]GGT
SfiI 466 GGCCTTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 458 CTCAAG C^T[CT][GA]AG
SphI 1302 GCATGC GCATG^C
SspI 100 AATATT AAT^ATT
TaqI 363 TCGA T^CGA
TthHB8I 363 TCGA T^CGA
XhoII 916 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 133 GACGCC G[GA]^CG[CT]C
1044 GACGTC
AlwI 917 GATCC GGATC(4/5)
916 GGATC
AspHI 569 GAGCTC G[AT]GC[AT]^C
684 GTGCAC
BalI 213 TGGCCA TGG^CCA
408 TGGCCA
BciVI 523 GGATAC GTATCC(6/5)
1308 GTATCC
BglI 175 GCCGGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
467 GCCTTGGAGGC
BsaHI 133 GACGCC G[GA]^CG[CT]C
1044 GACGTC
BsiHKAI 569 GAGCTC G[AT]GC[AT]^C
684 GTGCAC
BsrDI 590 GCAATG GCAATG(2/0)
776 GCAATG
BstAPI 304 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
396 GCAGGGGGTGC
BstF5I 1055 GGATG GGATG(2/0)
1116 GGATG
DpnII 917 GATC ^GATC
1279 GATC
EaeI 213 TGGCCA [CT]^GGCC[GA]
408 TGGCCA
FokI 1055 GGATG GGATG(9/13)
1116 GGATG
Hin1I 133 GACGCC G[GA]^CG[CT]C
1044 GACGTC
KspI 384 CCGCGG CCGC^GG
1008 CCGCGG
MaeII 1045 ACGT A^CGT
1263 ACGT
MaeIII 782 GTGAC ^GT[ACGT]AC
890 GTGAC
MboI 917 GATC ^GATC
1279 GATC
MluNI 213 TGGCCA TGG^CCA
408 TGGCCA
MscI 213 TGGCCA TGG^CCA
408 TGGCCA
MseI 1061 TTAA T^TAA
1088 TTAA
NdeII 917 GATC ^GATC
1279 GATC
SacII 384 CCGCGG CCGC^GG
1008 CCGCGG
Sau3AI 917 GATC ^GATC
1279 GATC
SmaI 979 CCCGGG CCC^GGG
1385 CCCGGG
Tru9I 1061 TTAA T^TAA
1088 TTAA
Tsp45I 782 GTGAC ^GT[GC]AC
890 GTGAC
XmaCI 979 CCCGGG C^CCGGG
1385 CCCGGG
XmaI 979 CCCGGG C^CCGGG
1385 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstNI BstUI Cac8I
Cfr13I DdeI DraII DsaI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PflMI PleI PstI Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI Tsp509I TspRI Van91I XcmI