##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaII       AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
CelII       ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        Hin1I       HincII      
HindII      HpaI        HphI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AocI          328  CCTCAGG              CC^T[ACGT]AGG
ApaI          323  GGGCCC               GGGCC^C
BanI          271  GGCACC               G^G[CT][GA]CC
BanII         323  GGGCCC               G[GA]GC[CT]^C
BbsI          187  GTCTTC               GAAGAC(2/6)
BpmI          135  CTCCAG               CTGGAG(16/14)
BpuAI         187  GTCTTC               GAAGAC(2/6)
BseRI         292  GAGGAG               GAGGAG(10/8)
BsgI          349  GTGCAG               GTGCAG(16/14)
BsmAI         343  GAGAC                GTCTC(1/5)
BsmBI         343  GAGACG               CGTCTC(1/5)
BspMI         152  ACCTGC               ACCTGC(4/8)
BsrFI         278  ACCGGC               [GA]^CCGG[CT]
BsrI          284  CCAGT                ACTGG(1/-1)
BstF5I        125  CATCC                GGATG(2/0)
Bsu36I        328  CCTCAGG              CC^T[ACGT]AGG
CfoI           31  GCGC                 GCG^C
Cfr10I        278  ACCGGC               [GA]^CCGG[CT]
DdeI          329  CTCAG                C^T[ACGT]AG
DraII         323  GGGCCCC              [GA]G^G[ACGT]CC[CT]
Eco81I        328  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      323  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       338  CCAAGG               C^C[AT][AT]GG
FauI           56  CCCGC                CCCGC(4/6)
FokI          125  CATCC                GGATG(9/13)
HaeII          30  GGCGCT               [GA]GCGC^[CT]
HgaI          156  GCGTC                GACGC(5/10)
HhaI           31  GCGC                 GCG^C
HinP1I         31  GCGC                 G^CGC
HindIII       244  AAGCTT               A^AGCTT
HinfI         334  GACTC                G^A[ACGT]TC
MboII         188  TCTTC                GAAGA(8/7)
MspA1I         57  CCGCTG               C[AC]G^C[GT]G
NlaIII        307  CATG                 CATG^
PleI          334  GACTC                GAGTC(4/5)
StyI          338  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AvaI           10  CCCGGG               C^[CT]CG[GA]G
              320  CCCGGG              
BmyI          270  GGGCAC               G[AGT]GC[ACT]^C
              323  GGGCCC              
BsoBI          10  CCCGGG               C^[CT]CG[GA]G
              320  CCCGGG              
Bsp1286I      270  GGGCAC               G[AGT]GC[ACT]^C
              323  GGGCCC              
BstNI          71  CCTGG                CC^[AT]GG
              213  CCTGG               
EcoRII         71  CCTGG                ^CC[AT]GG
              213  CCTGG               
MvaI           71  CCTGG                CC^[AT]GG
              213  CCTGG               
SmaI           10  CCCGGG               CCC^GGG
              320  CCCGGG              
XmaCI          10  CCCGGG               C^CCGGG
              320  CCCGGG              
XmaI           10  CCCGGG               C^CCGGG
              320  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AluI        BbvI        BcnI        BsaJI       
BsiYI       BslI        BssKI       Cac8I       Cfr13I      
Fnu4HI      HaeIII      HapII       HpaII       ItaI        
MnlI        MspI        MwoI        NciI        NlaIV       
Sau96I      ScrFI       TseI        TspRI