Restriction map for
pF1KB5711
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MaeI MluI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 1649 TCCGGA T^CCGGA
AflIII 2321 ACATGT A^C[GA][CT]GT
Aor51HI 369 AGCGCT AGC^GCT
Asp700I 153 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 403 CCCGAG C^[CT]CG[GA]G
BbsI 1892 GAAGAC GAAGAC(2/6)
BclI 2265 TGATCA T^GATCA
BpuAI 1892 GAAGAC GAAGAC(2/6)
BsaBI 2155 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1459 GAGACC GGTCTC(1/5)
BsaWI 1649 TCCGGA [AT]^CCGG[AT]
BseAI 1649 TCCGGA T^CCGGA
BsgI 2006 CTGCAC GTGCAG(16/14)
BsoBI 403 CCCGAG C^[CT]CG[GA]G
BspEI 1649 TCCGGA T^CCGGA
BspHI 1203 TCATGA T^CATGA
BspLU11I 2321 ACATGT A^CATGT
BstYI 1702 GGATCT [GA]^GATC[CT]
Eco47III 369 AGCGCT AGC^GCT
EcoRV 433 GATATC GAT^ATC
EcoT14I 1448 CCATGG C^C[AT][AT]GG
FauI 1970 GCGGG CCCGC(4/6)
FbaI 2265 TGATCA T^GATCA
HaeII 369 AGCGCT [GA]GCGC^[CT]
HindIII 208 AAGCTT A^AGCTT
MamI 2155 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1241 CAATTG C^AATTG
MflI 1702 GGATCT [GA]^GATC[CT]
MroI 1649 TCCGGA T^CCGGA
MspA1I 141 CAGCTG C[AC]G^C[GT]G
MunI 1241 CAATTG C^AATTG
NcoI 1448 CCATGG C^CATGG
NspI 2321 ACATGT [GA]CATG^[CT]
PflMI 1531 CCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 141 CAGCTG CAG^CTG
RcaI 1203 TCATGA T^CATGA
SacI 362 GAGCTC GAGCT^C
SalI 2081 GTCGAC G^TCGAC
StyI 1448 CCATGG C^C[AT][AT]GG
Van91I 1531 CCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1702 GGATCT [GA]^GATC[CT]
XmnI 153 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 574 GTCTAC GT^[AC][GT]AC
2081 GTCGAC
AccII 1741 CGCG CG^CG
2316 CGCG
AlwNI 740 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
2219 CAGTTGCTG
AspHI 362 GAGCTC G[AT]GC[AT]^C
2168 GTGCTC
BpmI 2060 CTCCAG CTGGAG(16/14)
169 CTGGAG
BsiHKAI 362 GAGCTC G[AT]GC[AT]^C
2168 GTGCTC
BsrDI 1360 GCAATG GCAATG(2/0)
1781 GCAATG
BstUI 1741 CGCG CG^CG
2316 CGCG
Cac8I 342 GCAGGC GC[ACGT]^[ACGT]GC
1807 GCTGGC
CfoI 138 GCGC GCG^C
370 GCGC
DraI 2092 TTTAAA TTT^AAA
2305 TTTAAA
EarI 1131 CTCTTC CTCTTC(1/4)
1391 GAAGAG
HhaI 138 GCGC GCG^C
370 GCGC
HinP1I 138 GCGC G^CGC
370 GCGC
Ksp632I 1131 CTCTTC CTCTTC(1/4)
1391 GAAGAG
MaeII 809 ACGT A^CGT
2024 ACGT
MslI 214 CACTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1737 CATCCGCGTG
MvnI 1741 CGCG CG^CG
2316 CGCG
NdeI 551 CATATG CA^TATG
2195 CATATG
PshAI 621 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1461 GACCACCGTC
PstI 34 CTGCAG CTGCA^G
2188 CTGCAG
SspI 1276 AATATT AAT^ATT
1945 AATATT
StuI 344 AGGCCT AGG^CCT
1790 AGGCCT
XcmI 317 CCACCAAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
386 CCAACGAACCCGTGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BalI BanII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MluNI MnlI MscI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I