Restriction map for
pF1KE2523
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# NO CUT ENZYMES ( 87 enzymes) #
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AccIII AclI AflII AgeI Alw44I
AocI ApaI ApaLI AscI AvrII
BamHI BanII BbeI BbrPI BbvCI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BsiWI BspEI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I CpoI DrdI EagI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FseI KasI MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I RsrII SacI
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspI StuI SwaI XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 592 GACGTC GACGT^C
AccI 2332 GTCTAC GT^[AC][GT]AC
Aor51HI 3689 AGCGCT AGC^GCT
AseI 2281 ATTAAT AT^TAAT
AsnI 2281 ATTAAT AT^TAAT
AspHI 2989 GTGCTC G[AT]GC[AT]^C
AspI 1241 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2925 TGCGCA TGC^GCA
BcgI 1659 CGATGAAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3270 GGATAC GTATCC(6/5)
BclI 2699 TGATCA T^GATCA
BglII 2180 AGATCT A^GATCT
BpmI 2195 CTGGAG CTGGAG(16/14)
BsaAI 884 TACGTG [CT]AC^GT[GA]
BsaWI 1796 ACCGGA [AT]^CCGG[AT]
BsiEI 207 CGATCG CG[GA][CT]^CG
BsiHKAI 2989 GTGCTC G[AT]GC[AT]^C
BsmBI 590 GAGACG CGTCTC(1/5)
BspDI 3129 ATCGAT AT^CGAT
BspHI 1388 TCATGA T^CATGA
BspLU11I 3366 ACATGT A^CATGT
BstAPI 2338 GCAAAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 3129 ATCGAT AT^CGAT
DraIII 2375 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3689 AGCGCT AGC^GCT
FbaI 2699 TGATCA T^GATCA
FspI 2925 TGCGCA TGC^GCA
HpaI 3438 GTTAAC GTT^AAC
KspI 3725 CCGCGG CCGC^GG
NdeI 1496 CATATG CA^TATG
NspI 3366 ACATGT [GA]CATG^[CT]
PaeR7I 2431 CTCGAG C^TCGAG
PflFI 1241 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2281 ATTAAT AT^TAAT
PstI 2794 CTGCAG CTGCA^G
PvuI 207 CGATCG CGAT^CG
PvuII 626 CAGCTG CAG^CTG
RcaI 1388 TCATGA T^CATGA
SacII 3725 CCGCGG CCGC^GG
SapI 376 GCTCTTC GCTCTTC(1/4)
SmlI 2431 CTCGAG C^T[CT][GA]AG
SpeI 432 ACTAGT A^CTAGT
Tth111I 1241 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2431 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
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Acc65I 1112 GGTACC G^GTACC
1197 GGTACC
AflIII 529 ACGTGT A^C[GA][CT]GT
3366 ACATGT
AlwNI 663 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
938 CAGGTGCTG
Asp718I 1112 GGTACC G^GTACC
1197 GGTACC
AvaI 1333 CTCGGG C^[CT]CG[GA]G
2431 CTCGAG
BalI 2666 TGGCCA TGG^CCA
3511 TGGCCA
BglI 2026 GCCAGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3706 GCCATCGTGGC
BsgI 2356 GTGCAG GTGCAG(16/14)
2577 GTGCAG
BsmI 2744 GAATGC GAATGC(1/-1)
3294 GAATGC
BsoBI 1333 CTCGGG C^[CT]CG[GA]G
2431 CTCGAG
Bsp1407I 184 TGTACA T^GTACA
2972 TGTACA
BsrDI 3225 CATTGC GCAATG(2/0)
3318 CATTGC
BsrGI 184 TGTACA T^GTACA
2972 TGTACA
EcoRV 1930 GATATC GAT^ATC
2789 GATATC
EcoT14I 1193 CCATGG C^C[AT][AT]GG
2621 CCATGG
FauI 1297 CCCGC CCCGC(4/6)
3642 GCGGG
HaeII 3614 GGCGCT [GA]GCGC^[CT]
3689 AGCGCT
KpnI 1112 GGTACC GGTAC^C
1197 GGTACC
MluNI 2666 TGGCCA TGG^CCA
3511 TGGCCA
MscI 2666 TGGCCA TGG^CCA
3511 TGGCCA
NcoI 1193 CCATGG C^CATGG
2621 CCATGG
PflMI 498 CCATAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2374 CCACCCGGTGG
SexAI 476 ACCTGGT A^CC[AT]GGT
2633 ACCTGGT
SspBI 184 TGTACA T^GTACA
2972 TGTACA
StyI 1193 CCATGG C^C[AT][AT]GG
2621 CCATGG
Van91I 498 CCATAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2374 CCACCCGGTGG
XcmI 1733 CCATCAGTGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2712 CCACATTGGCGTTGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI ApoI Asp700I
AspEI AvaII BanI BbsI BbvI
BcnI BfaI BmyI BpuAI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI Eam1105I
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI