Restriction map for
pF1KE2666
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# NO CUT ENZYMES ( 193 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BcgI BclI BcnI BfaI BfrI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DdeI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FbaI Fnu4HI FokI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HphI ItaI KasI KpnI
Ksp632I KspI MaeI MfeI MluI
MroI MspA1I MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmeI PpuMI
PshAI PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TaqI TseI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AciI 462 CCGC CCGC(-3/-1)
AclI 268 AACGTT AA^CGTT
AfaI 450 GTAC GT^AC
AflIII 686 ACGTGT A^C[GA][CT]GT
AseI 481 ATTAAT AT^TAAT
AsnI 481 ATTAAT AT^TAAT
Asp700I 265 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 729 GGTCC G^G[AT]CC
BalI 87 TGGCCA TGG^CCA
BbrPI 685 CACGTG CAC^GTG
BciVI 609 GGATAC GTATCC(6/5)
BsaAI 685 CACGTG [CT]AC^GT[GA]
BsaBI 325 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 732 CCGAGG C^C[ACGT][ACGT]GG
BssKI 90 CCAGG ^CC[ACGT]GG
BstNI 90 CCAGG CC^[AT]GG
Cfr13I 729 GGTCC G^G[ACGT]CC
EaeI 87 TGGCCA [CT]^GGCC[GA]
EcoRI 558 GAATTC G^AATTC
EcoRII 90 CCAGG ^CC[AT]GG
FauI 461 CCCGC CCCGC(4/6)
HapII 714 CCGG C^CGG
HinfI 223 GAATC G^A[ACGT]TC
HpaII 714 CCGG C^CGG
MamI 325 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 87 TGGCCA TGG^CCA
MscI 87 TGGCCA TGG^CCA
MslI 34 CATGTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 714 CCGG C^CGG
MvaI 90 CCAGG CC^[AT]GG
NlaIV 93 GGAACC GG[ACGT]^[ACGT]CC
PmaCI 685 CACGTG CAC^GTG
PmlI 685 CACGTG CAC^GTG
PshBI 481 ATTAAT AT^TAAT
Psp1406I 268 AACGTT AA^CGTT
RsaI 450 GTAC GT^AC
Sau96I 729 GGTCC G^G[ACGT]CC
ScrFI 90 CCAGG CC^[ACGT]GG
TfiI 223 GAATC G^A[AT]TC
Tsp45I 565 GTGAC ^GT[GC]AC
TspRI 709 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 265 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AcsI 176 AAATTC [GA]^AATT[CT]
558 GAATTC
AlwI 331 GATCC GGATC(4/5)
319 GGATC
ApoI 176 AAATTC [GA]^AATT[CT]
558 GAATTC
BglII 12 AGATCT A^GATCT
324 AGATCT
BstYI 12 AGATCT [GA]^GATC[CT]
324 AGATCT
HaeIII 3 GGCC GG^CC
88 GGCC
MaeII 269 ACGT A^CGT
686 ACGT
MflI 12 AGATCT [GA]^GATC[CT]
324 AGATCT
NlaIII 34 CATG CATG^
453 CATG
SmlI 208 CTCAAG C^T[CT][GA]AG
287 CTCAAG
SspI 55 AATATT AAT^ATT
697 AATATT
XhoII 12 AGATCT [GA]^GATC[CT]
324 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AluI DpnII MaeIII MboI MboII
MnlI MseI NdeII Sau3AI Tru9I
Tsp509I