Restriction map for
pF1KE2656
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# NO CUT ENZYMES ( 173 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanII BbeI BbrPI
BbvCI BcgI BclI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseAI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI Ksp632I KspI MaeI
MaeII MamI MfeI MluI MroI
MslI MspA1I MunI MvnI MwoI
NarI NcoI NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tsp45I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 419 GGTACC G^GTACC
Asp718I 419 GGTACC G^GTACC
BalI 81 TGGCCA TGG^CCA
BanI 419 GGTACC G^G[CT][GA]CC
BbsI 773 GTCTTC GAAGAC(2/6)
BbvI 396 GCAGC GCAGC(8/12)
BciVI 533 GTATCC GTATCC(6/5)
BcnI 544 CCGGG CC^[GC]GG
BpuAI 773 GTCTTC GAAGAC(2/6)
BsaJI 323 CCTGGG C^C[ACGT][ACGT]GG
BsaWI 416 TCCGGT [AT]^CCGG[AT]
BseRI 548 GAGGAG GAGGAG(10/8)
BsgI 782 CTGCAC GTGCAG(16/14)
BsmFI 632 GGGAC GGGAC(10/14)
BsrI 476 ACTGG ACTGG(1/-1)
BstF5I 639 CATCC GGATG(2/0)
BstNI 323 CCTGG CC^[AT]GG
BstXI 742 CCAAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 24 GGCCC G^G[ACGT]CC
EaeI 81 TGGCCA [CT]^GGCC[GA]
EcoRII 323 CCTGG ^CC[AT]GG
EcoT22I 647 ATGCAT ATGCA^T
FauI 14 GCGGG CCCGC(4/6)
Fnu4HI 396 GCAGC GC^[ACGT]GC
FokI 639 CATCC GGATG(9/13)
ItaI 396 GCAGC GC^[ACGT]GC
KpnI 419 GGTACC GGTAC^C
MaeIII 134 GTTAC ^GT[ACGT]AC
MluNI 81 TGGCCA TGG^CCA
MscI 81 TGGCCA TGG^CCA
MvaI 323 CCTGG CC^[AT]GG
NaeI 37 GCCGGC GCC^GGC
NciI 544 CCGGG CC^[GC]GG
NdeI 179 CATATG CA^TATG
NgoMIV 37 GCCGGC G^CCGGC
NlaIII 438 CATG CATG^
NsiI 647 ATGCAT ATGCA^T
PflMI 160 CCAATGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 394 CTGCAG CTGCA^G
Sau96I 24 GGCCC G^G[ACGT]CC
ScaI 406 AGTACT AGT^ACT
SfaNI 646 GATGC GCATC(5/9)
TfiI 520 GATTC G^A[AT]TC
TseI 396 GCAGC G^C[AT]GC
Van91I 160 CCAATGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 537 CCGC CCGC(-3/-1)
14 GCGG
AcsI 198 AAATTC [GA]^AATT[CT]
616 AAATTT
AfaI 407 GTAC GT^AC
420 GTAC
AlwI 272 GATCC GGATC(4/5)
96 GGATC
ApoI 198 AAATTC [GA]^AATT[CT]
616 AAATTT
BsmI 503 GCATTC GAATGC(1/-1)
539 GCATTC
BsrDI 236 CATTGC GCAATG(2/0)
91 GCAATG
BsrFI 37 GCCGGC [GA]^CCGG[CT]
497 GCCGGT
BssKI 323 CCTGG ^CC[ACGT]GG
544 CCGGG
BstYI 96 GGATCT [GA]^GATC[CT]
271 AGATCC
Cfr10I 37 GCCGGC [GA]^CCGG[CT]
497 GCCGGT
HaeIII 24 GGCC GG^CC
82 GGCC
HphI 327 GGTGA GGTGA(8/7)
44 TCACC
MboII 481 GAAGA GAAGA(8/7)
774 TCTTC
MflI 96 GGATCT [GA]^GATC[CT]
271 AGATCC
NlaIV 24 GGCCCC GG[ACGT]^[ACGT]CC
419 GGTACC
PleI 19 GAGTC GAGTC(4/5)
350 GAGTC
RsaI 407 GTAC GT^AC
420 GTAC
ScrFI 323 CCTGG CC^[ACGT]GG
544 CCGGG
SfcI 262 CTACAG C^T[GA][CT]AG
394 CTGCAG
Tsp509I 199 AATT ^AATT
617 AATT
XhoII 96 GGATCT [GA]^GATC[CT]
271 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI AlwNI BsiYI BslI Cac8I
DdeI DpnII HapII HinfI HpaII
MboI MnlI MseI MspI NdeII
Sau3AI Tru9I TspRI