Restriction map for
pF1KE2317
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# NO CUT ENZYMES ( 73 enzymes) #
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Acc65I AccI AclI AflII AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
BbvCI BfrI BglII BsiWI BsmI
BspDI Bst1107I BstAPI BstBI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I Eco81I EcoRV EcoT22I FseI
FspI HincII HindII HpaI KpnI
KspI MfeI MluI MseI MunI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I RsrII SacII SalI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspI StuI SwaI
Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1939 GACGTC GACGT^C
AccIII 1943 TCCGGA T^CCGGA
AcsI 2392 GAATTC [GA]^AATT[CT]
AflIII 362 ACATGT A^C[GA][CT]GT
ApoI 2392 GAATTC [GA]^AATT[CT]
Asp700I 2115 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2836 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2445 CCTAGG C^CTAGG
BamHI 2631 GGATCC G^GATCC
BbrPI 2913 CACGTG CAC^GTG
BclI 3023 TGATCA T^GATCA
BlnI 2445 CCTAGG C^CTAGG
BsaBI 315 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1943 TCCGGA T^CCGGA
Bsp1407I 1196 TGTACA T^GTACA
BspEI 1943 TCCGGA T^CCGGA
BspHI 1034 TCATGA T^CATGA
BspLU11I 362 ACATGT A^CATGT
BsrGI 1196 TGTACA T^GTACA
BssHII 20 GCGCGC G^CGCGC
BstEII 2172 GGTGACC G^GT[ACGT]ACC
BstPI 2172 GGTGACC G^GT[ACGT]ACC
BstYI 2631 GGATCC [GA]^GATC[CT]
EcoNI 1788 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2172 GGTGACC G^GT[ACGT]ACC
EcoRI 2392 GAATTC G^AATTC
FbaI 3023 TGATCA T^GATCA
HindIII 2316 AAGCTT A^AGCTT
MamI 315 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2631 GGATCC [GA]^GATC[CT]
MroI 1943 TCCGGA T^CCGGA
NdeI 829 CATATG CA^TATG
PflFI 2836 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2913 CACGTG CAC^GTG
PmlI 2913 CACGTG CAC^GTG
PshAI 2436 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1743 CGATCG CGAT^CG
RcaI 1034 TCATGA T^CATGA
SapI 3111 GAAGAGC GCTCTTC(1/4)
SbfI 397 CCTGCAGG CCTGCA^GG
SmlI 2383 CTCAAG C^T[CT][GA]AG
Sse8387I 397 CCTGCAGG CCTGCA^GG
SspBI 1196 TGTACA T^GTACA
Tth111I 2836 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2888 CCACCATCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2631 GGATCC [GA]^GATC[CT]
XmnI 2115 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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Aor51HI 197 AGCGCT AGC^GCT
1991 AGCGCT
AspHI 229 GAGCTC G[AT]GC[AT]^C
1169 GAGCTC
BcgI 146 GCAGCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2807 GCACCTTCATCG
BciVI 105 GGATAC GTATCC(6/5)
2493 GTATCC
BsaAI 487 TACGTG [CT]AC^GT[GA]
2913 CACGTG
BsaWI 257 ACCGGA [AT]^CCGG[AT]
1943 TCCGGA
BsiHKAI 229 GAGCTC G[AT]GC[AT]^C
1169 GAGCTC
BsrBI 187 GAGCGG CCGCTC(-3/-3)
310 GAGCGG
BssSI 2011 CACGAG CACGAG(-5/-1)
2792 CACGAG
DraIII 1366 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
2728 CACTGTGTG
EarI 151 CTCTTC CTCTTC(1/4)
3111 GAAGAG
Eco47III 197 AGCGCT AGC^GCT
1991 AGCGCT
Ksp632I 151 CTCTTC CTCTTC(1/4)
3111 GAAGAG
NcoI 1251 CCATGG C^CATGG
2514 CCATGG
NspI 362 ACATGT [GA]CATG^[CT]
584 GCATGT
PflMI 1650 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2133 CCAGAGGATGG
PstI 398 CTGCAG CTGCA^G
2206 CTGCAG
SacI 229 GAGCTC GAGCT^C
1169 GAGCTC
TfiI 113 GATTC G^A[AT]TC
678 GATTC
Tsp509I 1949 AATT ^AATT
2393 AATT
Van91I 1650 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2133 CCAGAGGATGG
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# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XmaCI XmaI