Restriction map for
pF1KE2585
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AhdI AocI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BbeI BbrPI BbsI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaHI
BsaI BsgI BsiWI BspDI BspMI
BsrBI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstXI Bsu36I
CelII ClaI DraI DraIII Eam1105I
Eco47III Eco81I EcoO65I EcoRV EcoT22I
FseI HaeII Hin1I HincII HindII
HpaI KasI KpnI KspI MaeI
MfeI MluI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SmlI SnaBI SpeI Sse8387I SspI
StuI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 1200 GTATAC GT^[AC][GT]AC
AccIII 14 TCCGGA T^CCGGA
AflIII 1862 ACATGT A^C[GA][CT]GT
Alw44I 664 GTGCAC G^TGCAC
ApaI 2228 GGGCCC GGGCC^C
ApaLI 664 GTGCAC G^TGCAC
AspHI 664 GTGCAC G[AT]GC[AT]^C
AvaI 272 CCCGGG C^[CT]CG[GA]G
AviII 2247 TGCGCA TGC^GCA
BalI 2363 TGGCCA TGG^CCA
BanI 992 GGTGCC G^G[CT][GA]CC
BanII 2228 GGGCCC G[GA]GC[CT]^C
BbvCI 2232 CCTCAGC CCTCAGC(-5/-2)
BclI 2375 TGATCA T^GATCA
BsaAI 661 TACGTG [CT]AC^GT[GA]
BsaBI 2280 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 14 TCCGGA T^CCGGA
BsiEI 1465 CGGCCG CG[GA][CT]^CG
BsiHKAI 664 GTGCAC G[AT]GC[AT]^C
BsmBI 1495 GAGACG CGTCTC(1/5)
BsmI 1830 GAATGC GAATGC(1/-1)
BsoBI 272 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1394 TGTACA T^GTACA
BspEI 14 TCCGGA T^CCGGA
BspHI 1754 TCATGA T^CATGA
BspLU11I 1862 ACATGT A^CATGT
BsrDI 1590 CATTGC GCAATG(2/0)
BsrGI 1394 TGTACA T^GTACA
Bst1107I 1200 GTATAC GTA^TAC
BstZ17I 1200 GTATAC GTA^TAC
CpoI 2061 CGGACCG CG^G[AT]CCG
DrdI 2146 GACACCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1465 CGGCCG C^GGCCG
EclXI 1465 CGGCCG C^GGCCG
Eco52I 1465 CGGCCG C^GGCCG
EcoNI 609 CCTGCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1922 GAATTC G^AATTC
FbaI 2375 TGATCA T^GATCA
FspI 2247 TGCGCA TGC^GCA
HindIII 190 AAGCTT A^AGCTT
MamI 2280 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2363 TGGCCA TGG^CCA
MroI 14 TCCGGA T^CCGGA
MscI 2363 TGGCCA TGG^CCA
NdeI 1660 CATATG CA^TATG
PflMI 1882 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1246 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 868 CTGCAG CTGCA^G
RcaI 1754 TCATGA T^CATGA
RsrII 2061 CGGACCG CG^G[AT]CCG
SapI 2301 GAAGAGC GCTCTTC(1/4)
ScaI 2180 AGTACT AGT^ACT
SgrAI 243 CACCGGTG C[GA]^CCGG[CT]G
SmaI 272 CCCGGG CCC^GGG
SphI 575 GCATGC GCATG^C
SspBI 1394 TGTACA T^GTACA
TfiI 837 GAATC G^A[AT]TC
Van91I 1882 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 272 CCCGGG C^CCGGG
XmaI 272 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AgeI 244 ACCGGT A^CCGGT
2455 ACCGGT
AlwNI 56 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1883 CAGAGTCTG
Asp700I 132 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2436 GAATAAATTC
BseRI 2010 GAGGAG GAGGAG(10/8)
2071 GAGGAG
BsrFI 244 ACCGGT [GA]^CCGG[CT]
2455 ACCGGT
Cac8I 575 GCATGC GC[ACGT]^[ACGT]GC
870 GCAGGC
Cfr10I 244 ACCGGT [GA]^CCGG[CT]
2455 ACCGGT
DraII 2228 GGGCCCT [GA]G^G[ACGT]CC[CT]
2384 AGGGCCT
EaeI 1465 CGGCCG [CT]^GGCC[GA]
2363 TGGCCA
EcoO109I 2228 GGGCCCT [GA]G^G[ACGT]CC[CT]
2384 AGGGCCT
HgaI 1497 GACGC GACGC(5/10)
2122 GACGC
NcoI 2150 CCATGG C^CATGG
2273 CCATGG
NspI 575 GCATGC [GA]CATG^[CT]
1862 ACATGT
PinAI 244 ACCGGT A^CCGGT
2455 ACCGGT
XcmI 1659 CCATATGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2351 CCACCCTGACCGTGG
XmnI 132 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2436 GAATAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BamHI BbvI BcnI
BmyI BpmI BsaJI BsaWI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI CfoI
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII