##################################
# NO CUT ENZYMES (  189 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        Eam1105I    EarI        Eco47III    EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaI        HpaII       HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tsp45I      
Tsp509I     Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AfaI          104  GTAC                 GT^AC
AlwNI           4  CAGCGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           98  CCTTAGG              CC^T[ACGT]AGG
ApaI          150  GGGCCC               GGGCC^C
BanII         150  GGGCCC               G[GA]GC[CT]^C
BbvCI         289  GCTGAGG              CCTCAGC(-5/-2)
BmyI          150  GGGCCC               G[AGT]GC[ACT]^C
BsiEI         276  CGGCCG               CG[GA][CT]^CG
BsiYI         257  CCTATGGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          257  CCTATGGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      150  GGGCCC               G[AGT]GC[ACT]^C
BsrBI          75  CCGCTC               CCGCTC(-3/-3)
BsrI           36  ACTGG                ACTGG(1/-1)
BssSI         202  CTCGTG               CACGAG(-5/-1)
Bsu36I         98  CCTTAGG              CC^T[ACGT]AGG
DpnII          27  GATC                 ^GATC
DraIII        199  CACCTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          182  GACCCCAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          232  CCATGG               C^C[GA][CT]GG
EagI          276  CGGCCG               C^GGCCG
EclXI         276  CGGCCG               C^GGCCG
Eco52I        276  CGGCCG               C^GGCCG
Eco81I         98  CCTTAGG              CC^T[ACGT]AGG
EcoT14I       232  CCATGG               C^C[AT][AT]GG
FauI           74  CCCGC                CCCGC(4/6)
HindIII        59  AAGCTT               A^AGCTT
MboI           27  GATC                 ^GATC
MspA1I        175  CAGCTG               C[AC]G^C[GT]G
NcoI          232  CCATGG               C^CATGG
NdeII          27  GATC                 ^GATC
NlaIII        233  CATG                 CATG^
PvuII         175  CAGCTG               CAG^CTG
RsaI          104  GTAC                 GT^AC
Sau3AI         27  GATC                 ^GATC
SfaNI          91  GATGC                GCATC(5/9)
StyI          232  CCATGG               C^C[AT][AT]GG
TaqI          168  TCGA                 T^CGA
TthHB8I       168  TCGA                 T^CGA
XcmI          113  CCAACAGGGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    3 enzymes) #
###################################

DdeI           99  CTTAG                C^T[ACGT]AG
              290  CTGAG               
EaeI           44  TGGCCG               [CT]^GGCC[GA]
              276  CGGCCG              
TspRI          35  CACTG                CA[GC]TG[ACGT][ACGT]^
              285  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BpmI        
BsaJI       BsmFI       BssKI       BstF5I      BstNI       
Cfr13I      DraII       EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      ItaI        MboII       MnlI        
MvaI        MwoI        NlaIV       PpuMI       Sau96I      
ScrFI       TseI