Restriction map for
pF1KE2381
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# NO CUT ENZYMES ( 79 enzymes) #
##################################
AccII AccIII AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AviII AvrII BamHI BbeI BbrPI
BciVI BfrI BlnI BsaAI BsaBI
BseAI BsiWI BsmI BspEI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstUI BstZ17I Cfr10I CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoT22I FseI FspI
KasI KspI MamI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacII SalI SbfI
SexAI SfoI SgrAI SnaBI SphI
Sse8387I SspI SwaI XbaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 3143 GACGTC GACGT^C
Acc65I 2699 GGTACC G^GTACC
AccI 2942 GTCTAC GT^[AC][GT]AC
AflIII 1984 ACACGT A^C[GA][CT]GT
AocI 3212 CCTCAGG CC^T[ACGT]AGG
Asp700I 1939 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2699 GGTACC G^GTACC
AspI 3992 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 324 GCACAAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2840 TGATCA T^GATCA
BglI 3711 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2827 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2827 GCTCAGC GC^T[ACGT]AGC
BsmBI 921 GAGACG CGTCTC(1/5)
Bsp1407I 1812 TGTACA T^GTACA
BspDI 423 ATCGAT AT^CGAT
BspHI 2516 TCATGA T^CATGA
BsrGI 1812 TGTACA T^GTACA
BstBI 459 TTCGAA TT^CGAA
Bsu36I 3212 CCTCAGG CC^T[ACGT]AGG
CelII 2827 GCTCAGC GC^T[ACGT]AGC
ClaI 423 ATCGAT AT^CGAT
DraIII 4237 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3515 CCATGG C^C[GA][CT]GG
Eco81I 3212 CCTCAGG CC^T[ACGT]AGG
EcoRI 2953 GAATTC G^AATTC
FauI 959 GCGGG CCCGC(4/6)
FbaI 2840 TGATCA T^GATCA
HaeII 3862 AGCGCC [GA]GCGC^[CT]
HincII 2359 GTTAAC GT[CT]^[GA]AC
HindII 2359 GTTAAC GT[CT]^[GA]AC
HindIII 3593 AAGCTT A^AGCTT
HpaI 2359 GTTAAC GTT^AAC
KpnI 2699 GGTACC GGTAC^C
MfeI 1592 CAATTG C^AATTG
MslI 2347 CACCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1592 CAATTG C^AATTG
NcoI 3515 CCATGG C^CATGG
NspV 459 TTCGAA TT^CGAA
PflFI 3992 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4101 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2516 TCATGA T^CATGA
SacI 2020 GAGCTC GAGCT^C
SfiI 3710 GGCCACAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 459 TTCGAA TT^CGAA
SmaI 356 CCCGGG CCC^GGG
SpeI 2253 ACTAGT A^CTAGT
SspBI 1812 TGTACA T^GTACA
Tth111I 3992 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4101 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2813 CCAATCTGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 356 CCCGGG C^CCGGG
XmaI 356 CCCGGG C^CCGGG
XmnI 1939 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AclI 1875 AACGTT AA^CGTT
2732 AACGTT
AcyI 2939 GGCGTC G[GA]^CG[CT]C
3143 GACGTC
Alw44I 2485 GTGCAC G^TGCAC
3024 GTGCAC
ApaI 3718 GGGCCC GGGCC^C
4380 GGGCCC
ApaLI 2485 GTGCAC G^TGCAC
3024 GTGCAC
BanI 2419 GGTGCC G^G[CT][GA]CC
2699 GGTACC
BglII 898 AGATCT A^GATCT
2034 AGATCT
BsaHI 2939 GGCGTC G[GA]^CG[CT]C
3143 GACGTC
BsaWI 434 ACCGGA [AT]^CCGG[AT]
587 ACCGGA
BsgI 322 CTGCAC GTGCAG(16/14)
4183 GTGCAG
BsiEI 315 CGACCG CG[GA][CT]^CG
925 CGGTCG
BstAPI 1219 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3544 GCACCCTCTGC
BstEII 165 GGTGACC G^GT[ACGT]ACC
869 GGTCACC
BstPI 165 GGTGACC G^GT[ACGT]ACC
869 GGTCACC
BstXI 149 CCAAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2869 CCAGCTGTGTGG
CfoI 490 GCGC GCG^C
3863 GCGC
EcoO65I 165 GGTGACC G^GT[ACGT]ACC
869 GGTCACC
EcoRV 223 GATATC GAT^ATC
438 GATATC
HgaI 102 GCGTC GACGC(5/10)
2940 GCGTC
HhaI 490 GCGC GCG^C
3863 GCGC
Hin1I 2939 GGCGTC G[GA]^CG[CT]C
3143 GACGTC
HinP1I 490 GCGC G^CGC
3863 GCGC
PaeR7I 765 CTCGAG C^TCGAG
807 CTCGAG
Psp1406I 1875 AACGTT AA^CGTT
2732 AACGTT
SapI 3172 GAAGAGC GCTCTTC(1/4)
1675 GCTCTTC
XhoI 765 CTCGAG C^TCGAG
807 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BalI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII