Restriction map for
pF1KE2783
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanI BbeI BbsI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI KspI MaeI
MamI MfeI MflI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tth111I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 271 GTAGAC GT^[AC][GT]AC
AflIII 347 ACATGT A^C[GA][CT]GT
AlwI 192 GATCC GGATC(4/5)
ApaI 86 GGGCCC GGGCC^C
BanII 86 GGGCCC G[GA]GC[CT]^C
BbrPI 579 CACGTG CAC^GTG
BpmI 590 CTCCAG CTGGAG(16/14)
BsaAI 579 CACGTG [CT]AC^GT[GA]
BseRI 145 GAGGAG GAGGAG(10/8)
BspLU11I 347 ACATGT A^CATGT
BspMI 771 GCAGGT ACCTGC(4/8)
BsrFI 32 ACCGGC [GA]^CCGG[CT]
BstEII 774 GGTGACC G^GT[ACGT]ACC
BstPI 774 GGTGACC G^GT[ACGT]ACC
Cfr10I 32 ACCGGC [GA]^CCGG[CT]
DpnII 192 GATC ^GATC
DraIII 463 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 74 CGGCCG C^GGCCG
EarI 220 GAAGAG CTCTTC(1/4)
EclXI 74 CGGCCG C^GGCCG
Eco52I 74 CGGCCG C^GGCCG
EcoO65I 774 GGTGACC G^GT[ACGT]ACC
HaeII 39 GGCGCT [GA]GCGC^[CT]
Ksp632I 220 GAAGAG CTCTTC(1/4)
MboI 192 GATC ^GATC
MboII 220 GAAGA GAAGA(8/7)
MseI 440 TTAA T^TAA
NdeII 192 GATC ^GATC
PflMI 332 CCAGTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 579 CACGTG CAC^GTG
PmlI 579 CACGTG CAC^GTG
PpuMI 635 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 650 CAGCTG CAG^CTG
Sau3AI 192 GATC ^GATC
TaqI 143 TCGA T^CGA
Tru9I 440 TTAA T^TAA
Tsp509I 611 AATT ^AATT
TthHB8I 143 TCGA T^CGA
Van91I 332 CCAGTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 5 CCAGGCCCGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 28 CGCG CG^CG
72 CGCG
AlwNI 100 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
375 CAGCATCTG
AvaII 93 GGTCC G^G[AT]CC
636 GGACC
BalI 2 TGGCCA TGG^CCA
459 TGGCCA
BbvCI 372 CCTCAGC CCTCAGC(-5/-2)
717 CCTCAGC
BcgI 574 CGAGCCACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
543 GCAAGAGCTTCG
BmyI 86 GGGCCC G[AGT]GC[ACT]^C
430 GGGCAC
BsgI 381 CTGCAC GTGCAG(16/14)
170 GTGCAG
BsiEI 30 CGACCG CG[GA][CT]^CG
74 CGGCCG
Bsp1286I 86 GGGCCC G[AGT]GC[ACT]^C
430 GGGCAC
BsrBI 19 GAGCGG CCGCTC(-3/-3)
412 GAGCGG
BsrI 332 CCAGT ACTGG(1/-1)
399 CCAGT
BstUI 28 CGCG CG^CG
72 CGCG
DsaI 77 CCGTGG C^C[GA][CT]GG
764 CCACGG
FauI 125 CCCGC CCCGC(4/6)
727 CCCGC
HphI 45 GGTGA GGTGA(8/7)
774 GGTGA
MluNI 2 TGGCCA TGG^CCA
459 TGGCCA
MscI 2 TGGCCA TGG^CCA
459 TGGCCA
MslI 262 CACAGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
750 CATCCAGATG
MspA1I 264 CAGCGG C[AC]G^C[GT]G
650 CAGCTG
MvnI 28 CGCG CG^CG
72 CGCG
NspI 347 ACATGT [GA]CATG^[CT]
452 GCATGT
SfcI 198 CTACAG C^T[GA][CT]AG
495 CTATAG
SmaI 83 CCCGGG CCC^GGG
181 CCCGGG
XmaCI 83 CCCGGG C^CCGGG
181 CCCGGG
XmaI 83 CCCGGG C^CCGGG
181 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AvaI BbvI BcnI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MaeIII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI SfaNI TseI Tsp45I TspRI