Restriction map for
pF1KE2438
##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BbrPI BlnI BsaAI BsaBI BsiEI
BsiWI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV FseI
HindIII HpaI KpnI KspI MamI
MluI MvnI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SalI
SexAI SfuI SgrAI SnaBI SpeI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1300 GTCTAC GT^[AC][GT]AC
AccIII 5000 TCCGGA T^CCGGA
AclI 323 AACGTT AA^CGTT
AflII 838 CTTAAG C^TTAAG
Aor51HI 3422 AGCGCT AGC^GCT
BbeI 3851 GGCGCC GGCGC^C
BcgI 5111 GCAGAAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 838 CTTAAG C^TTAAG
BglII 1188 AGATCT A^GATCT
BsaWI 5000 TCCGGA [AT]^CCGG[AT]
BseAI 5000 TCCGGA T^CCGGA
BsmBI 4144 GAGACG CGTCTC(1/5)
Bsp1407I 308 TGTACA T^GTACA
BspDI 976 ATCGAT AT^CGAT
BspEI 5000 TCCGGA T^CCGGA
BspHI 2480 TCATGA T^CATGA
BsrFI 3475 GCCGGT [GA]^CCGG[CT]
BsrGI 308 TGTACA T^GTACA
BstXI 4913 CCAACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 3475 GCCGGT [GA]^CCGG[CT]
ClaI 976 ATCGAT AT^CGAT
DraIII 2020 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3422 AGCGCT AGC^GCT
EcoT22I 1078 ATGCAT ATGCA^T
KasI 3851 GGCGCC G^GCGCC
MroI 5000 TCCGGA T^CCGGA
NarI 3851 GGCGCC GG^CGCC
NcoI 2066 CCATGG C^CATGG
NdeI 929 CATATG CA^TATG
NsiI 1078 ATGCAT ATGCA^T
Psp1406I 323 AACGTT AA^CGTT
RcaI 2480 TCATGA T^CATGA
SbfI 3045 CCTGCAGG CCTGCA^GG
SfoI 3851 GGCGCC GGC^GCC
Sse8387I 3045 CCTGCAGG CCTGCA^GG
SspBI 308 TGTACA T^GTACA
SspI 5596 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 3851 GGCGCC G[GA]^CG[CT]C
4150 GACGCC
AflIII 1484 ACATGT A^C[GA][CT]GT
4224 ACATGT
AspI 1953 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3061 GACAAAGTC
BanI 2062 GGTGCC G^G[CT][GA]CC
3851 GGCGCC
BsaHI 3851 GGCGCC G[GA]^CG[CT]C
4150 GACGCC
BsmI 4473 GAATGC GAATGC(1/-1)
3320 GCATTC
BspLU11I 1484 ACATGT A^CATGT
4224 ACATGT
BsrBI 3391 GAGCGG CCGCTC(-3/-3)
5356 GAGCGG
DsaI 1820 CCGTGG C^C[GA][CT]GG
2066 CCATGG
HgaI 2652 GACGC GACGC(5/10)
4150 GACGC
Hin1I 3851 GGCGCC G[GA]^CG[CT]C
4150 GACGCC
HincII 376 GTCAAC GT[CT]^[GA]AC
3067 GTCAAC
HindII 376 GTCAAC GT[CT]^[GA]AC
3067 GTCAAC
MfeI 593 CAATTG C^AATTG
3325 CAATTG
MunI 593 CAATTG C^AATTG
3325 CAATTG
PflFI 1953 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3061 GACAAAGTC
SacI 1573 GAGCTC GAGCT^C
5131 GAGCTC
ScaI 581 AGTACT AGT^ACT
1370 AGTACT
SfiI 3396 GGCCTCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5280 GGCCAAGAAGGCC
SmaI 3401 CCCGGG CCC^GGG
3437 CCCGGG
Tth111I 1953 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3061 GACAAAGTC
XbaI 820 TCTAGA T^CTAGA
3771 TCTAGA
XmaCI 3401 CCCGGG C^CCGGG
3437 CCCGGG
XmaI 3401 CCCGGG C^CCGGG
3437 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AvaI AvaII AviII BalI BanII
BbsI BbvCI BbvI BciVI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI FspI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI