Restriction map for
pF1KE2597
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# NO CUT ENZYMES ( 61 enzymes) #
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AatII AclI AcyI AflII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI AspEI BbeI BbrPI
BfrI BsaAI BsaHI BsiEI BsiWI
BsmBI Bst1107I BstBI BstZ17I CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV FseI Hin1I
HpaI KasI KspI MluI NarI
NdeI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SfoI SfuI SgrAI SnaBI
SwaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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Acc65I 766 GGTACC G^GTACC
Asp700I 2130 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 766 GGTACC G^GTACC
AvrII 1213 CCTAGG C^CTAGG
BciVI 4965 GGATAC GTATCC(6/5)
BlnI 1213 CCTAGG C^CTAGG
BsaBI 3687 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssHII 3844 GCGCGC G^CGCGC
BstEII 1326 GGTCACC G^GT[ACGT]ACC
BstPI 1326 GGTCACC G^GT[ACGT]ACC
BstXI 4907 CCAACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2014 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1326 GGTCACC G^GT[ACGT]ACC
EcoT22I 1078 ATGCAT ATGCA^T
HgaI 4197 GCGTC GACGC(5/10)
KpnI 766 GGTACC GGTAC^C
MamI 3687 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 593 CAATTG C^AATTG
MunI 593 CAATTG C^AATTG
NaeI 1780 GCCGGC GCC^GGC
NcoI 2060 CCATGG C^CATGG
NgoMIV 1780 GCCGGC G^CCGGC
NsiI 1078 ATGCAT ATGCA^T
PaeR7I 3915 CTCGAG C^TCGAG
PshAI 105 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 3039 CCTGCAGG CCTGCA^GG
SexAI 5609 ACCTGGT A^CC[AT]GGT
SpeI 3900 ACTAGT A^CTAGT
SphI 3597 GCATGC GCATG^C
Sse8387I 3039 CCTGCAGG CCTGCA^GG
StuI 692 AGGCCT AGG^CCT
XhoI 3915 CTCGAG C^TCGAG
XmnI 2130 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 39 enzymes) #
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AccI 1300 GTCTAC GT^[AC][GT]AC
5528 GTCTAC
AccII 3760 CGCG CG^CG
3845 CGCG
AccIII 3143 TCCGGA T^CCGGA
4994 TCCGGA
AflIII 1484 ACATGT A^C[GA][CT]GT
4218 ACATGT
Aor51HI 323 AGCGCT AGC^GCT
3416 AGCGCT
AviII 3533 TGCGCA TGC^GCA
4688 TGCGCA
BamHI 755 GGATCC G^GATCC
5705 GGATCC
BcgI 3696 CGATGACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5105 GCAGGAAAATCG
BsaI 3182 GAGACC GGTCTC(1/5)
4871 GAGACC
BseAI 3143 TCCGGA T^CCGGA
4994 TCCGGA
BsmI 4233 GAATGC GAATGC(1/-1)
4467 GAATGC
Bsp1407I 308 TGTACA T^GTACA
1268 TGTACA
BspDI 2218 ATCGAT AT^CGAT
3694 ATCGAT
BspEI 3143 TCCGGA T^CCGGA
4994 TCCGGA
BspHI 2474 TCATGA T^CATGA
4295 TCATGA
BspLU11I 1484 ACATGT A^CATGT
4218 ACATGT
BsrFI 1780 GCCGGC [GA]^CCGG[CT]
3469 GCCGGT
BsrGI 308 TGTACA T^GTACA
1268 TGTACA
BssSI 292 CACGAG CACGAG(-5/-1)
5713 CACGAG
BstAPI 589 GCAACAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1276 GCAGTGGGTGC
BstUI 3760 CGCG CG^CG
3845 CGCG
Cfr10I 1780 GCCGGC [GA]^CCGG[CT]
3469 GCCGGT
ClaI 2218 ATCGAT AT^CGAT
3694 ATCGAT
Eco47III 323 AGCGCT AGC^GCT
3416 AGCGCT
FspI 3533 TGCGCA TGC^GCA
4688 TGCGCA
HindIII 3247 AAGCTT A^AGCTT
4415 AAGCTT
MaeII 1534 ACGT A^CGT
5681 ACGT
MroI 3143 TCCGGA T^CCGGA
4994 TCCGGA
MvnI 3760 CGCG CG^CG
3845 CGCG
RcaI 2474 TCATGA T^CATGA
4295 TCATGA
SacI 1573 GAGCTC GAGCT^C
5125 GAGCTC
SapI 2593 GAAGAGC GCTCTTC(1/4)
5778 GAAGAGC
ScaI 581 AGTACT AGT^ACT
1370 AGTACT
SfiI 3390 GGCCTCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5274 GGCCAAGAAGGCC
SmaI 3395 CCCGGG CCC^GGG
3431 CCCGGG
SspBI 308 TGTACA T^GTACA
1268 TGTACA
SspI 3869 AATATT AAT^ATT
5590 AATATT
XmaCI 3395 CCCGGG C^CCGGG
3431 CCCGGG
XmaI 3395 CCCGGG C^CCGGG
3431 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoII