Restriction map for
pF1KE2468
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BbeI BbrPI BbvCI BciVI BclI
BfrI BglII BlnI BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrDI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 388 GGCGTC G[GA]^CG[CT]C
AlwNI 211 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 302 AGCGCT AGC^GCT
ApaI 928 GGGCCC GGGCC^C
AviII 332 TGCGCA TGC^GCA
BamHI 1267 GGATCC G^GATCC
BlpI 12 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 12 GCTGAGC GC^T[ACGT]AGC
BsaHI 388 GGCGTC G[GA]^CG[CT]C
BsaI 998 GAGACC GGTCTC(1/5)
BsaWI 917 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1463 TGTACA T^GTACA
BsrFI 502 GCCGGC [GA]^CCGG[CT]
BsrGI 1463 TGTACA T^GTACA
BssSI 739 CTCGTG CACGAG(-5/-1)
BstAPI 1320 GCACGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1200 GGTAACC G^GT[ACGT]ACC
BstPI 1200 GGTAACC G^GT[ACGT]ACC
BstYI 1267 GGATCC [GA]^GATC[CT]
CelII 12 GCTGAGC GC^T[ACGT]AGC
Cfr10I 502 GCCGGC [GA]^CCGG[CT]
DraI 841 TTTAAA TTT^AAA
Eco47III 302 AGCGCT AGC^GCT
EcoO65I 1200 GGTAACC G^GT[ACGT]ACC
EcoT14I 533 CCAAGG C^C[AT][AT]GG
FseI 501 GGCCGGCC GGCCGG^CC
FspI 332 TGCGCA TGC^GCA
HgaI 389 GCGTC GACGC(5/10)
Hin1I 388 GGCGTC G[GA]^CG[CT]C
HincII 1630 GTCAAC GT[CT]^[GA]AC
HindII 1630 GTCAAC GT[CT]^[GA]AC
MflI 1267 GGATCC [GA]^GATC[CT]
MspA1I 1459 CCGCTG C[AC]G^C[GT]G
NaeI 502 GCCGGC GCC^GGC
NgoMIV 502 GCCGGC G^CCGGC
PstI 904 CTGCAG CTGCA^G
SfcI 904 CTGCAG C^T[GA][CT]AG
SspBI 1463 TGTACA T^GTACA
StuI 1274 AGGCCT AGG^CCT
StyI 533 CCAAGG C^C[AT][AT]GG
XhoII 1267 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 642 CGCG CG^CG
942 CGCG
Alw44I 147 GTGCAC G^TGCAC
1383 GTGCAC
ApaLI 147 GTGCAC G^TGCAC
1383 GTGCAC
AvaI 995 CCCGAG C^[CT]CG[GA]G
1278 CTCGGG
AvaII 682 GGACC G^G[AT]CC
735 GGACC
BanII 928 GGGCCC G[GA]GC[CT]^C
1312 GAGCCC
BbsI 589 GAAGAC GAAGAC(2/6)
1609 GTCTTC
BcgI 882 CGAGAAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
731 GCAAGGACCTCG
BfaI 865 CTAG C^TAG
1240 CTAG
BpuAI 589 GAAGAC GAAGAC(2/6)
1609 GTCTTC
BseRI 340 CTCCTC GAGGAG(10/8)
910 GAGGAG
BsgI 313 CTGCAC GTGCAG(16/14)
362 CTGCAC
BsoBI 995 CCCGAG C^[CT]CG[GA]G
1278 CTCGGG
BspMI 311 ACCTGC ACCTGC(4/8)
228 GCAGGT
BsrBI 61 CCGCTC CCGCTC(-3/-3)
598 GAGCGG
BstUI 642 CGCG CG^CG
942 CGCG
BstXI 776 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1079 CCATCTTTGTGG
DraIII 1321 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1377 CACTGAGTG
DsaI 290 CCGTGG C^C[GA][CT]GG
431 CCACGG
EarI 205 CTCTTC CTCTTC(1/4)
603 GAAGAG
HaeII 9 GGCGCT [GA]GCGC^[CT]
302 AGCGCT
Ksp632I 205 CTCTTC CTCTTC(1/4)
603 GAAGAG
MaeI 865 CTAG C^TAG
1240 CTAG
MseI 842 TTAA T^TAA
1130 TTAA
MvnI 642 CGCG CG^CG
942 CGCG
NspI 260 ACATGC [GA]CATG^[CT]
779 ACATGC
PflMI 137 CCATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
210 CCAGATTCTGG
PpuMI 681 GGGACCC [GA]G^G[AT]CC[CT]
734 AGGACCT
SmlI 895 CTCAAG C^T[CT][GA]AG
1558 CTCAAG
TfiI 213 GATTC G^A[AT]TC
833 GATTC
Tru9I 842 TTAA T^TAA
1130 TTAA
Tsp509I 838 AATT ^AATT
1602 AATT
Van91I 137 CCATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
210 CCAGATTCTGG
XcmI 431 CCACGGCCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1649 CCATATTTGACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BalI BanI BbvI BcnI BglI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I TspRI