Restriction map for
pF1KE8021
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BfrI BlnI BsaHI BsaI BseAI
BsgI BsiWI BspDI BspEI BspLU11I
BspMI BsrBI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Bsu36I CfoI ClaI CpoI DraI
DrdI DsaI Eam1105I Eco47III Eco81I
EcoO65I EcoT14I FseI FspI HaeII
HhaI Hin1I HinP1I KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NruI NspV
PacI PflFI PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 687 GTATAC GT^[AC][GT]AC
AgeI 18 ACCGGT A^CCGGT
AlwNI 160 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 252 GGGCCC GGGCC^C
Asp700I 1678 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1948 GAGCTC G[AT]GC[AT]^C
AvaI 1523 CTCGAG C^[CT]CG[GA]G
BamHI 552 GGATCC G^GATCC
BbvCI 1692 CCTCAGC CCTCAGC(-5/-2)
BcgI 1364 CGAAGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 534 GTATCC GTATCC(6/5)
BclI 2007 TGATCA T^GATCA
BcnI 81 CCGGG CC^[GC]GG
BglII 1809 AGATCT A^GATCT
BlpI 1855 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1855 GCTGAGC GC^T[ACGT]AGC
BsaBI 1440 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 18 ACCGGT [AT]^CCGG[AT]
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsiHKAI 1948 GAGCTC G[AT]GC[AT]^C
BsmBI 1502 CGTCTC CGTCTC(1/5)
BsoBI 1523 CTCGAG C^[CT]CG[GA]G
BsrFI 18 ACCGGT [GA]^CCGG[CT]
Bst1107I 687 GTATAC GTA^TAC
BstZ17I 687 GTATAC GTA^TAC
CelII 1855 GCTGAGC GC^T[ACGT]AGC
Cfr10I 18 ACCGGT [GA]^CCGG[CT]
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoNI 2151 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 426 GAATTC G^AATTC
EcoT22I 895 ATGCAT ATGCA^T
FbaI 2007 TGATCA T^GATCA
HgaI 1501 GCGTC GACGC(5/10)
HincII 570 GTTAAC GT[CT]^[GA]AC
HindII 570 GTTAAC GT[CT]^[GA]AC
HindIII 1643 AAGCTT A^AGCTT
HpaI 570 GTTAAC GTT^AAC
MamI 1440 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 81 CCGGG CC^[GC]GG
NdeI 1470 CATATG CA^TATG
NotI 4 GCGGCCGC GC^GGCCGC
NsiI 895 ATGCAT ATGCA^T
NspI 522 GCATGC [GA]CATG^[CT]
PaeR7I 1523 CTCGAG C^TCGAG
PinAI 18 ACCGGT A^CCGGT
PpuMI 1428 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1377 CTGCAG CTGCA^G
PvuII 1996 CAGCTG CAG^CTG
SacI 1948 GAGCTC GAGCT^C
SapI 271 GAAGAGC GCTCTTC(1/4)
SbfI 1376 CCTGCAGG CCTGCA^GG
ScaI 666 AGTACT AGT^ACT
SmlI 1523 CTCGAG C^T[CT][GA]AG
SphI 522 GCATGC GCATG^C
Sse8387I 1376 CCTGCAGG CCTGCA^GG
SspI 865 AATATT AAT^ATT
Tsp45I 1784 GTGAC ^GT[GC]AC
XhoI 1523 CTCGAG C^TCGAG
XmnI 1678 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BbsI 220 GAAGAC GAAGAC(2/6)
2093 GTCTTC
BpmI 473 CTCCAG CTGGAG(16/14)
1136 CTGGAG
BpuAI 220 GAAGAC GAAGAC(2/6)
2093 GTCTTC
BsaAI 559 CACGTA [CT]AC^GT[GA]
605 TACGTG
Bsp1407I 800 TGTACA T^GTACA
1236 TGTACA
BspHI 1224 TCATGA T^CATGA
1907 TCATGA
BsrGI 800 TGTACA T^GTACA
1236 TGTACA
EaeI 5 CGGCCG [CT]^GGCC[GA]
1474 TGGCCG
FauI 277 CCCGC CCCGC(4/6)
1845 GCGGG
HapII 19 CCGG C^CGG
81 CCGG
HpaII 19 CCGG C^CGG
81 CCGG
MslI 624 CACTAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1704 CACCCAGGTG
MspI 19 CCGG C^CGG
81 CCGG
RcaI 1224 TCATGA T^CATGA
1907 TCATGA
SfcI 1325 CTACAG C^T[GA][CT]AG
1377 CTGCAG
SspBI 800 TGTACA T^GTACA
1236 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BbvI BfaI
BglI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DraIII EarI EcoO109I EcoRII
EcoRV Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII