Restriction map for
pF1KE1913
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AocI Aor51HI
ApoI AscI AseI AsnI Asp718I
AvrII BbeI BbrPI BbvCI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI HindIII HpaI KasI KpnI
MaeI MamI MfeI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I XbaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 145 GTCTAC GT^[AC][GT]AC
AcyI 1279 GACGCC G[GA]^CG[CT]C
AhdI 1249 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 13 GTGCAC G^TGCAC
ApaLI 13 GTGCAC G^TGCAC
Asp700I 492 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1249 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 220 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 749 TGCGCA TGC^GCA
BamHI 1031 GGATCC G^GATCC
BanI 771 GGTGCC G^G[CT][GA]CC
BbsI 1312 GAAGAC GAAGAC(2/6)
BciVI 474 GTATCC GTATCC(6/5)
BpmI 130 CTGGAG CTGGAG(16/14)
BpuAI 1312 GAAGAC GAAGAC(2/6)
BsaHI 1279 GACGCC G[GA]^CG[CT]C
BsaI 592 GAGACC GGTCTC(1/5)
BsmAI 592 GAGAC GTCTC(1/5)
BsmFI 668 GGGAC GGGAC(10/14)
BspHI 488 TCATGA T^CATGA
BspMI 746 ACCTGC ACCTGC(4/8)
BstXI 188 CCATTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1031 GGATCC [GA]^GATC[CT]
DdeI 231 CTCAG C^T[ACGT]AG
Eam1105I 1249 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 24 GGCCGGCC GGCCGG^CC
FspI 749 TGCGCA TGC^GCA
HaeII 615 GGCGCT [GA]GCGC^[CT]
Hin1I 1279 GACGCC G[GA]^CG[CT]C
HincII 763 GTCAAC GT[CT]^[GA]AC
HindII 763 GTCAAC GT[CT]^[GA]AC
KspI 1074 CCGCGG CCGC^GG
MflI 1031 GGATCC [GA]^GATC[CT]
NcoI 215 CCATGG C^CATGG
NspI 959 GCATGT [GA]CATG^[CT]
PaeR7I 182 CTCGAG C^TCGAG
PflFI 220 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 668 GGGACCT [GA]G^G[AT]CC[CT]
PstI 388 CTGCAG CTGCA^G
RcaI 488 TCATGA T^CATGA
SacI 862 GAGCTC GAGCT^C
SacII 1074 CCGCGG CCGC^GG
SbfI 387 CCTGCAGG CCTGCA^GG
SfcI 388 CTGCAG C^T[GA][CT]AG
Sse8387I 387 CCTGCAGG CCTGCA^GG
TfiI 340 GATTC G^A[AT]TC
Tsp509I 265 AATT ^AATT
Tth111I 220 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 182 CTCGAG C^TCGAG
XhoII 1031 GGATCC [GA]^GATC[CT]
XmnI 492 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 985 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1123 CAGGAGCTG
ApaI 845 GGGCCC GGGCC^C
1341 GGGCCC
AspHI 13 GTGCAC G[AT]GC[AT]^C
862 GAGCTC
BcgI 38 GCAACCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1013 GCAGCTACTTCG
BglI 29 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
386 GCCTGCAGGGC
BsaAI 106 TACGTG [CT]AC^GT[GA]
175 TACGTG
BsgI 787 CTGCAC GTGCAG(16/14)
1183 CTGCAC
BsiHKAI 13 GTGCAC G[AT]GC[AT]^C
862 GAGCTC
BsrFI 25 GCCGGC [GA]^CCGG[CT]
289 GCCGGC
Cfr10I 25 GCCGGC [GA]^CCGG[CT]
289 GCCGGC
EagI 921 CGGCCG C^GGCCG
1286 CGGCCG
EclXI 921 CGGCCG C^GGCCG
1286 CGGCCG
Eco52I 921 CGGCCG C^GGCCG
1286 CGGCCG
NaeI 25 GCCGGC GCC^GGC
289 GCCGGC
NgoMIV 25 GCCGGC G^CCGGC
289 GCCGGC
SexAI 680 ACCTGGT A^CC[AT]GGT
1250 ACCTGGT
SmaI 726 CCCGGG CCC^GGG
824 CCCGGG
XmaCI 726 CCCGGG C^CCGGG
824 CCCGGG
XmaI 726 CCCGGG C^CCGGG
824 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BalI BanII BbvI
BcnI BmyI BsaJI BseRI BsiEI
BsiYI BslI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI