Restriction map for
pF1KE2664
##################################
# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BseRI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstNI
BstPI BstZ17I Bsu36I Cac8I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI MaeI MaeII MaeIII
MfeI MluI MluNI MroI MscI
MseI MunI MvaI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tru9I Tsp45I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AlwI 54 GATCC GGATC(4/5)
AlwNI 52 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
BanII 40 GGGCTC G[GA]GC[CT]^C
BbsI 168 GTCTTC GAAGAC(2/6)
BcnI 38 CCGGG CC^[GC]GG
BpmI 317 CTGGAG CTGGAG(16/14)
BpuAI 168 GTCTTC GAAGAC(2/6)
BsaBI 264 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 402 GGTCTC GGTCTC(1/5)
BsaJI 76 CCGCGG C^C[ACGT][ACGT]GG
BsgI 305 GTGCAG GTGCAG(16/14)
BsiEI 35 CGGCCG CG[GA][CT]^CG
BsmAI 403 GTCTC GTCTC(1/5)
BsrBI 15 GAGCGG CCGCTC(-3/-3)
BssKI 38 CCGGG ^CC[ACGT]GG
BstAPI 307 GCAGCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 255 CATCC GGATG(2/0)
BstXI 130 CCAGAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 53 AGATCC [GA]^GATC[CT]
CfoI 6 GCGC GCG^C
DpnII 54 GATC ^GATC
DraII 91 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 76 CCGCGG C^C[GA][CT]GG
EaeI 35 CGGCCG [CT]^GGCC[GA]
EagI 35 CGGCCG C^GGCCG
EclXI 35 CGGCCG C^GGCCG
Eco52I 35 CGGCCG C^GGCCG
EcoO109I 91 GGGACCC [GA]G^G[ACGT]CC[CT]
FokI 255 CATCC GGATG(9/13)
HapII 38 CCGG C^CGG
HgaI 392 GCGTC GACGC(5/10)
HhaI 6 GCGC GCG^C
HinP1I 6 GCGC G^CGC
HinfI 293 GAATC G^A[ACGT]TC
HpaII 38 CCGG C^CGG
HphI 212 TCACC GGTGA(8/7)
KspI 76 CCGCGG CCGC^GG
MamI 264 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 54 GATC ^GATC
MflI 53 AGATCC [GA]^GATC[CT]
MslI 226 CACAAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 38 CCGG C^CGG
NciI 38 CCGGG CC^[GC]GG
NdeII 54 GATC ^GATC
PpuMI 91 GGGACCC [GA]G^G[AT]CC[CT]
PstI 46 CTGCAG CTGCA^G
SacII 76 CCGCGG CCGC^GG
Sau3AI 54 GATC ^GATC
ScrFI 38 CCGGG CC^[ACGT]GG
SfcI 46 CTGCAG C^T[GA][CT]AG
TfiI 293 GAATC G^A[AT]TC
Tsp509I 282 AATT ^AATT
XhoII 53 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccII 7 CGCG CG^CG
77 CGCG
AluI 309 AGCT AG^CT
423 AGCT
AvaII 92 GGACC G^G[AT]CC
277 GGACC
BstUI 7 CGCG CG^CG
77 CGCG
DdeI 58 CTGAG C^T[ACGT]AG
217 CTCAG
EarI 175 CTCTTC CTCTTC(1/4)
208 CTCTTC
FauI 29 GCGGG CCCGC(4/6)
78 GCGGG
HaeIII 36 GGCC GG^CC
204 GGCC
Ksp632I 175 CTCTTC CTCTTC(1/4)
208 CTCTTC
MspA1I 24 CAGCGG C[AC]G^C[GT]G
76 CCGCGG
MvnI 7 CGCG CG^CG
77 CGCG
NlaIII 184 CATG CATG^
243 CATG
SfaNI 254 GCATC GCATC(5/9)
269 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI BbvI BmyI BsiYI BslI
BsmFI Bsp1286I BsrI Cfr13I Fnu4HI
ItaI MboII MnlI MwoI NlaIV
Sau96I TseI TspRI