Restriction map for
pF1KE2496
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI AlwI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I CelII
ClaI CpoI DraI Eam1105I Eco47III
EcoO65I EcoRV EcoT22I FbaI HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI NarI NcoI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SapI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccII 446 CGCG CG^CG
Alw44I 1686 GTGCAC G^TGCAC
AocI 1342 CCTCAGG CC^T[ACGT]AGG
ApaLI 1686 GTGCAC G^TGCAC
AviII 734 TGCGCA TGC^GCA
BfaI 545 CTAG C^TAG
BsaI 1044 GGTCTC GGTCTC(1/5)
BsaWI 1412 TCCGGT [AT]^CCGG[AT]
BsrDI 476 GCAATG GCAATG(2/0)
BstUI 446 CGCG CG^CG
Bsu36I 1342 CCTCAGG CC^T[ACGT]AGG
DraIII 49 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1633 CCGTGG C^C[GA][CT]GG
Eco81I 1342 CCTCAGG CC^T[ACGT]AGG
EcoRI 1876 GAATTC G^AATTC
FseI 757 GGCCGGCC GGCCGG^CC
FspI 734 TGCGCA TGC^GCA
HgaI 834 GACGC GACGC(5/10)
HindIII 366 AAGCTT A^AGCTT
MaeI 545 CTAG C^TAG
MvnI 446 CGCG CG^CG
NheI 544 GCTAGC G^CTAGC
PflMI 682 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 700 GTCGAC G^TCGAC
SbfI 1083 CCTGCAGG CCTGCA^GG
SfiI 820 GGCCCAAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1318 CTCAAG C^T[CT][GA]AG
Sse8387I 1083 CCTGCAGG CCTGCA^GG
Van91I 682 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 39 enzymes) #
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AatII 1162 GACGTC GACGT^C
1513 GACGTC
AccI 700 GTCGAC GT^[AC][GT]AC
1171 GTCTAC
AcsI 503 AAATTT [GA]^AATT[CT]
1876 GAATTC
AlwNI 620 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
683 CAGCCTCTG
ApaI 62 GGGCCC GGGCC^C
819 GGGCCC
ApoI 503 AAATTT [GA]^AATT[CT]
1876 GAATTC
AspI 694 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1513 GACGTCGTC
BbsI 301 GAAGAC GAAGAC(2/6)
1944 GTCTTC
BglI 316 GCCGGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
821 GCCCAAGGGGC
BpmI 1564 CTGGAG CTGGAG(16/14)
1714 CTGGAG
BpuAI 301 GAAGAC GAAGAC(2/6)
1944 GTCTTC
BsiEI 756 CGGCCG CG[GA][CT]^CG
1806 CGGCCG
BsmAI 1045 GTCTC GTCTC(1/5)
1205 GTCTC
Bsp1407I 8 TGTACA T^GTACA
971 TGTACA
BsrGI 8 TGTACA T^GTACA
971 TGTACA
BstXI 76 CCATGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
194 CCATCTACCTGG
DpnII 459 GATC ^GATC
498 GATC
DrdI 1036 GACCATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1162 GACGTCGGGGTC
EagI 756 CGGCCG C^GGCCG
1806 CGGCCG
EarI 184 CTCTTC CTCTTC(1/4)
247 CTCTTC
EclXI 756 CGGCCG C^GGCCG
1806 CGGCCG
Eco52I 756 CGGCCG C^GGCCG
1806 CGGCCG
EcoT14I 452 CCAAGG C^C[AT][AT]GG
823 CCAAGG
HincII 421 GTCAAC GT[CT]^[GA]AC
700 GTCGAC
HindII 421 GTCAAC GT[CT]^[GA]AC
700 GTCGAC
Ksp632I 184 CTCTTC CTCTTC(1/4)
247 CTCTTC
MboI 459 GATC ^GATC
498 GATC
MseI 434 TTAA T^TAA
611 TTAA
NdeII 459 GATC ^GATC
498 GATC
PflFI 694 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1513 GACGTCGTC
SacI 1307 GAGCTC GAGCT^C
1764 GAGCTC
Sau3AI 459 GATC ^GATC
498 GATC
SphI 1386 GCATGC GCATG^C
1830 GCATGC
SspBI 8 TGTACA T^GTACA
971 TGTACA
StuI 977 AGGCCT AGG^CCT
1028 AGGCCT
StyI 452 CCAAGG C^C[AT][AT]GG
823 CCAAGG
Tru9I 434 TTAA T^TAA
611 TTAA
Tth111I 694 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1513 GACGTCGTC
XcmI 1241 CCATCATGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1928 CCATGCGACTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EaeI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboII MnlI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau96I ScrFI SfaNI SfcI
SmaI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XmaCI XmaI