##################################
# NO CUT ENZYMES (  196 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BseAI       
BsgI        BsiEI       BsiWI       BsmAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DdeI        DpnII       
DraI        DraII       DraIII      EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoT14I     EcoT22I     FbaI        FseI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HinfI       HpaI        KasI        KpnI        Ksp632I     
MaeII       MaeIII      MamI        MboI        MboII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MunI        MvaI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SalI        
SapI        Sau3AI      SbfI        ScaI        ScrFI       
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AvaI          130  CTCGGG               C^[CT]CG[GA]G
AvaII         150  GGACC                G^G[AT]CC
AviII         227  TGCGCA               TGC^GCA
BanI           87  GGTGCC               G^G[CT][GA]CC
BfaI          139  CTAG                 C^TAG
BpmI           34  CTGGAG               CTGGAG(16/14)
BsaJI         118  CCGCGG               C^C[ACGT][ACGT]GG
BsaWI          48  ACCGGA               [AT]^CCGG[AT]
BsiYI          42  CCTGACACCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           42  CCTGACACCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI          82  GGGAC                GGGAC(10/14)
BsoBI         130  CTCGGG               C^[CT]CG[GA]G
BssHII        125  GCGCGC               G^CGCGC
Cfr13I        150  GGACC                G^G[ACGT]CC
DrdI          160  GACAAGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          118  CCGCGG               C^C[GA][CT]GG
EaeI          209  TGGCCG               [CT]^GGCC[GA]
EcoRV         142  GATATC               GAT^ATC
FspI          227  TGCGCA               TGC^GCA
HapII          49  CCGG                 C^CGG
HpaII          49  CCGG                 C^CGG
HphI          155  TCACC                GGTGA(8/7)
KspI          118  CCGCGG               CCGC^GG
MaeI          139  CTAG                 C^TAG
MslI          225  CATGCGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           49  CCGG                 C^CGG
NlaIV          87  GGTGCC               GG[ACGT]^[ACGT]CC
PstI          253  CTGCAG               CTGCA^G
SacII         118  CCGCGG               CCGC^GG
Sau96I        150  GGACC                G^G[ACGT]CC
SfaNI         113  GCATC                GCATC(5/9)
SfcI          253  CTGCAG               C^T[GA][CT]AG
SmlI           58  CTCAAG               C^T[CT][GA]AG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AspHI          55  GAGCTC               G[AT]GC[AT]^C
              199  GAGCTC              
BanII          55  GAGCTC               G[GA]GC[CT]^C
              199  GAGCTC              
BmyI           55  GAGCTC               G[AGT]GC[ACT]^C
              199  GAGCTC              
BseRI          52  GAGGAG               GAGGAG(10/8)
              238  GAGGAG              
BsiHKAI        55  GAGCTC               G[AT]GC[AT]^C
              199  GAGCTC              
Bsp1286I       55  GAGCTC               G[AGT]GC[ACT]^C
              199  GAGCTC              
BstF5I         21  CATCC                GGATG(2/0)
              114  CATCC               
Cac8I         125  GCGCGC               GC[ACGT]^[ACGT]GC
              258  GCGGGC              
FokI           21  CATCC                GGATG(9/13)
              114  CATCC               
NlaIII        225  CATG                 CATG^
              231  CATG                
NspI          224  ACATGC               [GA]CATG^[CT]
              230  GCATGT              
SacI           55  GAGCTC               GAGCT^C
              199  GAGCTC              


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BstUI       
CfoI        FauI        Fnu4HI      HaeIII      HhaI        
HinP1I      ItaI        MnlI        MspA1I      MvnI        
MwoI        TseI