Restriction map for
pF1KE2340
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstXI Bsu36I CelII CfoI ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SbfI ScaI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 292 GACGTC GACGT^C
AccI 420 GTATAC GT^[AC][GT]AC
AcyI 292 GACGTC G[GA]^CG[CT]C
AlwI 336 GGATC GGATC(4/5)
Asp700I 205 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 352 GTGCTC G[AT]GC[AT]^C
AvaI 91 CTCGAG C^[CT]CG[GA]G
AvaII 251 GGTCC G^G[AT]CC
BanII 82 GAGCCC G[GA]GC[CT]^C
BbsI 451 GAAGAC GAAGAC(2/6)
BfaI 346 CTAG C^TAG
BpuAI 451 GAAGAC GAAGAC(2/6)
BsaHI 292 GACGTC G[GA]^CG[CT]C
BsiEI 60 CGGCCG CG[GA][CT]^CG
BsiHKAI 352 GTGCTC G[AT]GC[AT]^C
BsmAI 134 GAGAC GTCTC(1/5)
BsoBI 91 CTCGAG C^[CT]CG[GA]G
BsrI 137 ACTGG ACTGG(1/-1)
Bst1107I 420 GTATAC GTA^TAC
BstF5I 99 CATCC GGATG(2/0)
BstYI 336 GGATCT [GA]^GATC[CT]
BstZ17I 420 GTATAC GTA^TAC
DpnII 337 GATC ^GATC
DraII 465 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 435 CCATGG C^C[GA][CT]GG
EagI 60 CGGCCG C^GGCCG
EarI 408 CTCTTC CTCTTC(1/4)
EclXI 60 CGGCCG C^GGCCG
Eco52I 60 CGGCCG C^GGCCG
EcoNI 273 CCTTCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 465 GGGGCCT [GA]G^G[ACGT]CC[CT]
FokI 99 CATCC GGATG(9/13)
HgaI 380 GACGC GACGC(5/10)
Hin1I 292 GACGTC G[GA]^CG[CT]C
Ksp632I 408 CTCTTC CTCTTC(1/4)
MaeI 346 CTAG C^TAG
MaeII 293 ACGT A^CGT
MboI 337 GATC ^GATC
MflI 336 GGATCT [GA]^GATC[CT]
MspA1I 324 CCGCTG C[AC]G^C[GT]G
NcoI 435 CCATGG C^CATGG
NdeII 337 GATC ^GATC
NheI 345 GCTAGC G^CTAGC
PaeR7I 91 CTCGAG C^TCGAG
PflMI 456 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 487 GACTC GAGTC(4/5)
SapI 407 GCTCTTC GCTCTTC(1/4)
Sau3AI 337 GATC ^GATC
SexAI 305 ACCTGGT A^CC[AT]GGT
SmlI 91 CTCGAG C^T[CT][GA]AG
TaqI 92 TCGA T^CGA
Tsp509I 428 AATT ^AATT
TthHB8I 92 TCGA T^CGA
Van91I 456 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 91 CTCGAG C^TCGAG
XhoII 336 GGATCT [GA]^GATC[CT]
XmnI 205 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BcnI 58 CCCGG CC^[GC]GG
78 CCCGG
BglI 239 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
391 GCCCATCTGGC
BmyI 82 GAGCCC G[AGT]GC[ACT]^C
352 GTGCTC
BseRI 166 CTCCTC GAGGAG(10/8)
280 GAGGAG
BsmFI 29 GGGAC GGGAC(10/14)
252 GTCCC
Bsp1286I 82 GAGCCC G[AGT]GC[ACT]^C
352 GTGCTC
DdeI 116 CTGAG C^T[ACGT]AG
286 CTTAG
EaeI 8 TGGCCG [CT]^GGCC[GA]
60 CGGCCG
MaeIII 290 GTGAC ^GT[ACGT]AC
485 GTGAC
MboII 451 GAAGA GAAGA(8/7)
409 TCTTC
NciI 58 CCCGG CC^[GC]GG
78 CCCGG
NlaIII 112 CATG CATG^
436 CATG
TfiI 21 GATTC G^A[AT]TC
162 GAATC
Tsp45I 290 GTGAC ^GT[GC]AC
485 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsrFI BssKI BstNI Cac8I
Cfr10I Cfr13I EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MnlI MspI MvaI MwoI
NlaIV Sau96I ScrFI StyI TseI
TspRI