Restriction map for
pF1KE2576
##################################
# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HhaI HinP1I HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 451 GTCGAC GT^[AC][GT]AC
AcyI 490 GACGCC G[GA]^CG[CT]C
AflIII 413 ACATGT A^C[GA][CT]GT
AlwI 78 GATCC GGATC(4/5)
Asp700I 348 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 296 TGATCA T^GATCA
BmyI 336 GGGCAC G[AGT]GC[ACT]^C
BsaHI 490 GACGCC G[GA]^CG[CT]C
BsaWI 301 ACCGGA [AT]^CCGG[AT]
Bsp1286I 336 GGGCAC G[AGT]GC[ACT]^C
BspLU11I 413 ACATGT A^CATGT
BstYI 77 AGATCC [GA]^GATC[CT]
Cac8I 220 GCCAGC GC[ACGT]^[ACGT]GC
DraII 143 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 375 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 167 CCATGG C^C[GA][CT]GG
EcoO109I 143 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 167 CCATGG C^C[AT][AT]GG
FbaI 296 TGATCA T^GATCA
HapII 302 CCGG C^CGG
Hin1I 490 GACGCC G[GA]^CG[CT]C
HincII 451 GTCGAC GT[CT]^[GA]AC
HindII 451 GTCGAC GT[CT]^[GA]AC
HinfI 72 GACTC G^A[ACGT]TC
HpaII 302 CCGG C^CGG
MaeIII 383 GTGAC ^GT[ACGT]AC
MflI 77 AGATCC [GA]^GATC[CT]
MspA1I 222 CAGCGG C[AC]G^C[GT]G
MspI 302 CCGG C^CGG
MwoI 369 GCTGACCACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 167 CCATGG C^CATGG
NspI 413 ACATGT [GA]CATG^[CT]
PleI 72 GACTC GAGTC(4/5)
PpuMI 143 AGGACCT [GA]G^G[AT]CC[CT]
SalI 451 GTCGAC G^TCGAC
SfaNI 202 GATGC GCATC(5/9)
SmlI 271 CTCAAG C^T[CT][GA]AG
StuI 95 AGGCCT AGG^CCT
StyI 167 CCATGG C^C[AT][AT]GG
Tsp45I 383 GTGAC ^GT[GC]AC
XcmI 114 CCAGAACCGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 77 AGATCC [GA]^GATC[CT]
XmnI 348 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AocI 286 CCTGAGG CC^T[ACGT]AGG
325 CCTGAGG
BbsI 52 GTCTTC GAAGAC(2/6)
244 GTCTTC
BbvI 163 GCAGC GCAGC(8/12)
366 GCTGC
BpmI 45 CTCCAG CTGGAG(16/14)
358 CTGGAG
BpuAI 52 GTCTTC GAAGAC(2/6)
244 GTCTTC
BsmFI 152 GGGAC GGGAC(10/14)
227 GTCCC
BstF5I 201 GGATG GGATG(2/0)
291 GGATG
Bsu36I 286 CCTGAGG CC^T[ACGT]AGG
325 CCTGAGG
Eco81I 286 CCTGAGG CC^T[ACGT]AGG
325 CCTGAGG
FokI 201 GGATG GGATG(9/13)
291 GGATG
HgaI 490 GACGC GACGC(5/10)
50 GCGTC
HphI 239 TCACC GGTGA(8/7)
299 TCACC
TseI 163 GCAGC G^C[AT]GC
366 GCTGC
TspRI 102 CACTG CA[GC]TG[ACGT][ACGT]^
379 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI AvaII BanI BsaJI
BseRI BsiYI BslI BssKI BstNI
Cfr13I DdeI DpnII EcoRII Fnu4HI
HaeIII ItaI MaeII MboI MboII
MnlI MvaI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TaqI TthHB8I