Restriction map for
pF1KE3269
##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AlwI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AviII BalI
BamHI BbeI BciVI BfrI BsaBI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspHI BssHII Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MluNI
MroI MscI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI TfiI Tsp509I Van91I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AgeI 1865 ACCGGT A^CCGGT
AocI 1872 CCTCAGG CC^T[ACGT]AGG
AspI 490 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1128 CCTAGG C^CTAGG
BbvCI 1144 CCTCAGC CCTCAGC(-5/-2)
BcgI 797 GCACGTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 425 TGATCA T^GATCA
BlnI 1128 CCTAGG C^CTAGG
BsaWI 1865 ACCGGT [AT]^CCGG[AT]
BsiEI 1863 CGACCG CG[GA][CT]^CG
Bsp1407I 1598 TGTACA T^GTACA
BspLU11I 1273 ACATGT A^CATGT
BsrDI 1692 CATTGC GCAATG(2/0)
BsrGI 1598 TGTACA T^GTACA
BstAPI 1908 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1222 GGTGACC G^GT[ACGT]ACC
BstPI 1222 GGTGACC G^GT[ACGT]ACC
Bsu36I 1872 CCTCAGG CC^T[ACGT]AGG
EaeI 530 TGGCCG [CT]^GGCC[GA]
Eco81I 1872 CCTCAGG CC^T[ACGT]AGG
EcoNI 1404 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1222 GGTGACC G^GT[ACGT]ACC
FbaI 425 TGATCA T^GATCA
HaeII 649 AGCGCC [GA]GCGC^[CT]
KspI 208 CCGCGG CCGC^GG
MseI 1081 TTAA T^TAA
NaeI 563 GCCGGC GCC^GGC
NcoI 1217 CCATGG C^CATGG
NgoMIV 563 GCCGGC G^CCGGC
PflFI 490 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1865 ACCGGT A^CCGGT
PshAI 1839 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 208 CCGCGG CCGC^GG
SfiI 1180 GGCCACCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1955 CCCGGG CCC^GGG
SphI 227 GCATGC GCATG^C
SspBI 1598 TGTACA T^GTACA
Tru9I 1081 TTAA T^TAA
Tth111I 490 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1955 CCCGGG C^CCGGG
XmaI 1955 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1210 GTCTAC GT^[AC][GT]AC
1684 GTCTAC
AhdI 1087 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1753 GACATTGAGTC
Alw44I 795 GTGCAC G^TGCAC
1446 GTGCAC
ApaLI 795 GTGCAC G^TGCAC
1446 GTGCAC
AspEI 1087 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1753 GACATTGAGTC
BfaI 985 CTAG C^TAG
1129 CTAG
BglII 1151 AGATCT A^GATCT
1625 AGATCT
BlpI 645 GCTCAGC GC^T[ACGT]AGC
1038 GCTCAGC
Bpu1102I 645 GCTCAGC GC^T[ACGT]AGC
1038 GCTCAGC
BsaI 826 GAGACC GGTCTC(1/5)
1455 GGTCTC
BsrBI 252 CCGCTC CCGCTC(-3/-3)
276 CCGCTC
BsrFI 563 GCCGGC [GA]^CCGG[CT]
1865 ACCGGT
BssSI 664 CACGAG CACGAG(-5/-1)
895 CACGAG
BstYI 1151 AGATCT [GA]^GATC[CT]
1625 AGATCT
CelII 645 GCTCAGC GC^T[ACGT]AGC
1038 GCTCAGC
Cfr10I 563 GCCGGC [GA]^CCGG[CT]
1865 ACCGGT
Eam1105I 1087 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1753 GACATTGAGTC
MaeI 985 CTAG C^TAG
1129 CTAG
MflI 1151 AGATCT [GA]^GATC[CT]
1625 AGATCT
PpuMI 1046 AGGACCT [GA]G^G[AT]CC[CT]
1168 GGGACCT
PvuII 672 CAGCTG CAG^CTG
711 CAGCTG
SmlI 751 CTCAAG C^T[CT][GA]AG
1003 CTCAAG
StuI 685 AGGCCT AGG^CCT
1551 AGGCCT
XcmI 602 CCATCTTTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1710 CCACATTGTCTGTGG
XhoII 1151 AGATCT [GA]^GATC[CT]
1625 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbrPI
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PmaCI PmlI
PstI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I TspRI TthHB8I