Restriction map for
pF1KE9678
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspI AviII
BalI BanI BbeI BbrPI BcgI
BclI BglI BlpI Bpu1102I BsaAI
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspEI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII CfoI Cfr10I CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MluI MluNI
MroI MscI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RsrII SacII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AflII 4228 CTTAAG C^TTAAG
AhdI 1812 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1051 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1195 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1812 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 392 CCCGGG C^[CT]CG[GA]G
AvrII 1419 CCTAGG C^CTAGG
BamHI 4132 GGATCC G^GATCC
BciVI 396 GGATAC GTATCC(6/5)
BfrI 4228 CTTAAG C^TTAAG
BlnI 1419 CCTAGG C^CTAGG
BsoBI 392 CCCGGG C^[CT]CG[GA]G
BspDI 712 ATCGAT AT^CGAT
BspHI 2985 TCATGA T^CATGA
BsrDI 726 CATTGC GCAATG(2/0)
BstXI 2922 CCAAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 712 ATCGAT AT^CGAT
DraIII 2390 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2708 CCATGG C^C[GA][CT]GG
Eam1105I 1812 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4022 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1341 GAATTC G^AATTC
EcoRV 4098 GATATC GAT^ATC
HgaI 1084 GACGC GACGC(5/10)
HincII 3343 GTCGAC GT[CT]^[GA]AC
HindII 3343 GTCGAC GT[CT]^[GA]AC
HindIII 3742 AAGCTT A^AGCTT
MfeI 2837 CAATTG C^AATTG
MunI 2837 CAATTG C^AATTG
NcoI 2708 CCATGG C^CATGG
RcaI 2985 TCATGA T^CATGA
SacI 3940 GAGCTC GAGCT^C
SalI 3343 GTCGAC G^TCGAC
SmaI 392 CCCGGG CCC^GGG
XcmI 4175 CCAATAGCAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 392 CCCGGG C^CCGGG
XmaI 392 CCCGGG C^CCGGG
XmnI 1195 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 3343 GTCGAC GT^[AC][GT]AC
3559 GTAGAC
AocI 2548 CCTCAGG CC^T[ACGT]AGG
3769 CCTGAGG
AseI 1143 ATTAAT AT^TAAT
2803 ATTAAT
AsnI 1143 ATTAAT AT^TAAT
2803 ATTAAT
AspHI 1129 GAGCAC G[AT]GC[AT]^C
3940 GAGCTC
AvaII 969 GGACC G^G[AT]CC
3310 GGTCC
BanII 2734 GGGCTC G[GA]GC[CT]^C
3940 GAGCTC
BbvCI 1802 CCTCAGC CCTCAGC(-5/-2)
3934 GCTGAGG
BglII 1017 AGATCT A^GATCT
2306 AGATCT
BsaBI 308 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
705 GATTTGCATC
BsaWI 2472 TCCGGT [AT]^CCGG[AT]
2865 ACCGGA
BsgI 2033 CTGCAC GTGCAG(16/14)
2788 GTGCAG
BsiHKAI 1129 GAGCAC G[AT]GC[AT]^C
3940 GAGCTC
BsmAI 490 GAGAC GTCTC(1/5)
3624 GTCTC
BsmFI 1397 GGGAC GGGAC(10/14)
2409 GGGAC
BsmI 3103 GAATGC GAATGC(1/-1)
4243 GCATTC
BspLU11I 3303 ACATGT A^CATGT
4087 ACATGT
Bsu36I 2548 CCTCAGG CC^T[ACGT]AGG
3769 CCTGAGG
EaeI 1771 CGGCCA [CT]^GGCC[GA]
2489 CGGCCA
Eco81I 2548 CCTCAGG CC^T[ACGT]AGG
3769 CCTGAGG
MamI 308 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
705 GATTTGCATC
NdeI 2660 CATATG CA^TATG
4238 CATATG
NspI 3303 ACATGT [GA]CATG^[CT]
4087 ACATGT
PpuMI 968 AGGACCT [GA]G^G[AT]CC[CT]
3309 AGGTCCT
PshBI 1143 ATTAAT AT^TAAT
2803 ATTAAT
SfcI 25 CTACAG C^T[GA][CT]AG
1334 CTACAG
SspI 2242 AATATT AAT^ATT
3715 AATATT
StuI 1291 AGGCCT AGG^CCT
1465 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII