Restriction map for
pF1KB5968
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BcnI BglI BlnI BsaAI
BsaBI BsaI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmFI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT22I FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI NaeI NarI NciI
NdeI NgoMIV NotI NruI NsiI
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tth111I Van91I XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 1264 GACGTC GACGT^C
AccI 1521 GTCTAC GT^[AC][GT]AC
AccII 607 CGCG CG^CG
AflII 1527 CTTAAG C^TTAAG
ApaI 11 GGGCCC GGGCC^C
Asp700I 304 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 979 GAGCTC G[AT]GC[AT]^C
AvaI 515 CTCGAG C^[CT]CG[GA]G
BclI 1149 TGATCA T^GATCA
BfrI 1527 CTTAAG C^TTAAG
BglII 320 AGATCT A^GATCT
BlpI 634 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 634 GCTGAGC GC^T[ACGT]AGC
BsaWI 587 ACCGGA [AT]^CCGG[AT]
BsiHKAI 979 GAGCTC G[AT]GC[AT]^C
BsmI 291 GAATGC GAATGC(1/-1)
BsoBI 515 CTCGAG C^[CT]CG[GA]G
BspLU11I 364 ACATGT A^CATGT
BstBI 77 TTCGAA TT^CGAA
BstUI 607 CGCG CG^CG
BstYI 320 AGATCT [GA]^GATC[CT]
CelII 634 GCTGAGC GC^T[ACGT]AGC
CfoI 606 GCGC GCG^C
DraI 1570 TTTAAA TTT^AAA
DrdI 742 GACTCCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 802 GATATC GAT^ATC
EcoT14I 905 CCATGG C^C[AT][AT]GG
FauI 1044 GCGGG CCCGC(4/6)
FbaI 1149 TGATCA T^GATCA
HapII 588 CCGG C^CGG
HhaI 606 GCGC GCG^C
HinP1I 606 GCGC G^CGC
HpaII 588 CCGG C^CGG
MfeI 1060 CAATTG C^AATTG
MflI 320 AGATCT [GA]^GATC[CT]
MslI 349 CATTATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 588 CCGG C^CGG
MunI 1060 CAATTG C^AATTG
MvnI 607 CGCG CG^CG
NcoI 905 CCATGG C^CATGG
NheI 1449 GCTAGC G^CTAGC
NspI 364 ACATGT [GA]CATG^[CT]
NspV 77 TTCGAA TT^CGAA
PaeR7I 515 CTCGAG C^TCGAG
PstI 116 CTGCAG CTGCA^G
SacI 979 GAGCTC GAGCT^C
SbfI 115 CCTGCAGG CCTGCA^GG
SfuI 77 TTCGAA TT^CGAA
Sse8387I 115 CCTGCAGG CCTGCA^GG
StyI 905 CCATGG C^C[AT][AT]GG
XbaI 1179 TCTAGA T^CTAGA
XcmI 615 CCAGCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 515 CTCGAG C^TCGAG
XhoII 320 AGATCT [GA]^GATC[CT]
XmnI 304 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 364 ACATGT A^C[GA][CT]GT
1461 ACACGT
AocI 724 CCTGAGG CC^T[ACGT]AGG
1277 CCTTAGG
BanII 11 GGGCCC G[GA]GC[CT]^C
979 GAGCTC
BciVI 283 GGATAC GTATCC(6/5)
176 GTATCC
BfaI 1180 CTAG C^TAG
1450 CTAG
BsiYI 722 CCCCTGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
844 CCAGCATCAGG
BslI 722 CCCCTGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
844 CCAGCATCAGG
BsrDI 407 GCAATG GCAATG(2/0)
1651 GCAATG
Bsu36I 724 CCTGAGG CC^T[ACGT]AGG
1277 CCTTAGG
Cfr13I 11 GGGCC G^G[ACGT]CC
12 GGCCC
DraII 10 GGGGCCC [GA]G^G[ACGT]CC[CT]
11 GGGCCCC
DsaI 52 CCGTGG C^C[GA][CT]GG
905 CCATGG
EaeI 534 TGGCCG [CT]^GGCC[GA]
665 CGGCCA
EarI 1454 CTCTTC CTCTTC(1/4)
976 GAAGAG
Eco81I 724 CCTGAGG CC^T[ACGT]AGG
1277 CCTTAGG
EcoO109I 10 GGGGCCC [GA]G^G[ACGT]CC[CT]
11 GGGCCCC
HgaI 645 GACGC GACGC(5/10)
212 GCGTC
Ksp632I 1454 CTCTTC CTCTTC(1/4)
976 GAAGAG
MaeI 1180 CTAG C^TAG
1450 CTAG
PleI 742 GACTC GAGTC(4/5)
1216 GACTC
SapI 976 GAAGAGC GCTCTTC(1/4)
1453 GCTCTTC
Sau96I 11 GGGCC G^G[ACGT]CC
12 GGCCC
SfcI 116 CTGCAG C^T[GA][CT]AG
1468 CTACAG
Tsp45I 26 GTGAC ^GT[GC]AC
833 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
ApoI BbsI BbvI BmyI BpmI
BpuAI BsaHI BsaJI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII Hin1I HinfI HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI SfaNI SmlI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I