Restriction map for
pF1KE2611
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII HphI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XcmI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 227 GCGG CCGC(-3/-1)
AocI 7 CCTGAGG CC^T[ACGT]AGG
ApaI 747 GGGCCC GGGCC^C
Asp700I 291 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 820 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 653 CTCGAG C^[CT]CG[GA]G
BbsI 706 GTCTTC GAAGAC(2/6)
BfaI 571 CTAG C^TAG
BlpI 62 GCTTAGC GC^T[ACGT]AGC
BpmI 543 CTCCAG CTGGAG(16/14)
Bpu1102I 62 GCTTAGC GC^T[ACGT]AGC
BpuAI 706 GTCTTC GAAGAC(2/6)
BsaI 656 GAGACC GGTCTC(1/5)
BseRI 514 CTCCTC GAGGAG(10/8)
BsoBI 653 CTCGAG C^[CT]CG[GA]G
BssKI 618 CCTGG ^CC[ACGT]GG
BstNI 618 CCTGG CC^[AT]GG
Bsu36I 7 CCTGAGG CC^T[ACGT]AGG
CelII 62 GCTTAGC GC^T[ACGT]AGC
DraII 747 GGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 54 GAAGAG CTCTTC(1/4)
Eco81I 7 CCTGAGG CC^T[ACGT]AGG
EcoO109I 747 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 618 CCTGG ^CC[AT]GG
HindIII 30 AAGCTT A^AGCTT
Ksp632I 54 GAAGAG CTCTTC(1/4)
MaeI 571 CTAG C^TAG
MaeIII 242 GTCAC ^GT[ACGT]AC
MseI 830 TTAA T^TAA
MslI 213 CATTTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 254 CAGCTG C[AC]G^C[GT]G
MvaI 618 CCTGG CC^[AT]GG
PaeR7I 653 CTCGAG C^TCGAG
PflFI 820 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 324 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 254 CAGCTG CAG^CTG
SapI 54 GAAGAGC GCTCTTC(1/4)
ScrFI 618 CCTGG CC^[ACGT]GG
SfcI 740 CTGTAG C^T[GA][CT]AG
SmlI 653 CTCGAG C^T[CT][GA]AG
TfiI 735 GATTC G^A[AT]TC
Tru9I 830 TTAA T^TAA
Tsp45I 242 GTCAC ^GT[GC]AC
Tsp509I 302 AATT ^AATT
Tth111I 820 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 324 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 653 CTCGAG C^TCGAG
XmnI 291 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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BalI 209 TGGCCA TGG^CCA
500 TGGCCA
BsmFI 621 GGGAC GGGAC(10/14)
665 GTCCC
BsrI 45 ACTGG ACTGG(1/-1)
324 CCAGT
BstXI 212 CCATTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
545 CCAGAAACTTGG
Cac8I 223 GCTGGC GC[ACGT]^[ACGT]GC
688 GCAGGC
Cfr13I 747 GGGCC G^G[ACGT]CC
748 GGCCC
DpnII 411 GATC ^GATC
490 GATC
EaeI 209 TGGCCA [CT]^GGCC[GA]
500 TGGCCA
EcoT14I 114 CCTTGG C^C[AT][AT]GG
683 CCTTGG
MaeII 97 ACGT A^CGT
156 ACGT
MboI 411 GATC ^GATC
490 GATC
MboII 54 GAAGA GAAGA(8/7)
707 TCTTC
MluNI 209 TGGCCA TGG^CCA
500 TGGCCA
MscI 209 TGGCCA TGG^CCA
500 TGGCCA
NdeII 411 GATC ^GATC
490 GATC
PshAI 380 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
658 GACCTGAGTC
Sau3AI 411 GATC ^GATC
490 GATC
Sau96I 747 GGGCC G^G[ACGT]CC
748 GGCCC
StuI 567 AGGCCT AGG^CCT
774 AGGCCT
StyI 114 CCTTGG C^C[AT][AT]GG
683 CCTTGG
TaqI 150 TCGA T^CGA
654 TCGA
TthHB8I 150 TCGA T^CGA
654 TCGA
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI AlwNI BanII BbvI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrDI BstF5I DdeI Fnu4HI FokI
HaeIII HinfI ItaI MnlI NlaIII
NlaIV PleI TseI TspRI