Restriction map for
pF1KE2443
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseRI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI Ksp632I MamI MfeI
MluI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
TaqI TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 539 GTCTAC GT^[AC][GT]AC
AccIII 98 TCCGGA T^CCGGA
Alw44I 292 GTGCAC G^TGCAC
ApaLI 292 GTGCAC G^TGCAC
AspHI 292 GTGCAC G[AT]GC[AT]^C
AspI 936 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 857 GGGCTC G[GA]GC[CT]^C
BpmI 380 CTGGAG CTGGAG(16/14)
BsaI 353 GAGACC GGTCTC(1/5)
BsaWI 98 TCCGGA [AT]^CCGG[AT]
BseAI 98 TCCGGA T^CCGGA
BsgI 43 GTGCAG GTGCAG(16/14)
BsiHKAI 292 GTGCAC G[AT]GC[AT]^C
BsmBI 127 GAGACG CGTCTC(1/5)
BspEI 98 TCCGGA T^CCGGA
BspMI 902 ACCTGC ACCTGC(4/8)
BstF5I 826 GGATG GGATG(2/0)
DsaI 38 CCGCGG C^C[GA][CT]GG
EcoT14I 907 CCAAGG C^C[AT][AT]GG
FokI 826 GGATG GGATG(9/13)
HincII 78 GTTGAC GT[CT]^[GA]AC
HindII 78 GTTGAC GT[CT]^[GA]AC
KspI 38 CCGCGG CCGC^GG
MaeII 228 ACGT A^CGT
MroI 98 TCCGGA T^CCGGA
MseI 512 TTAA T^TAA
MspA1I 38 CCGCGG C[AC]G^C[GT]G
PflFI 936 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 38 CCGCGG CCGC^GG
SspI 650 AATATT AAT^ATT
StuI 191 AGGCCT AGG^CCT
StyI 907 CCAAGG C^C[AT][AT]GG
TfiI 888 GATTC G^A[AT]TC
Tru9I 512 TTAA T^TAA
Tsp45I 947 GTGAC ^GT[GC]AC
Tth111I 936 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 39 CGCG CG^CG
205 CGCG
BalI 113 TGGCCA TGG^CCA
645 TGGCCA
BamHI 71 GGATCC G^GATCC
101 GGATCC
BcnI 178 CCCGG CC^[GC]GG
734 CCCGG
BmyI 292 GTGCAC G[AGT]GC[ACT]^C
857 GGGCTC
BsmFI 673 GTCCC GGGAC(10/14)
732 GTCCC
Bsp1286I 292 GTGCAC G[AGT]GC[ACT]^C
857 GGGCTC
BstUI 39 CGCG CG^CG
205 CGCG
BstYI 71 GGATCC [GA]^GATC[CT]
101 GGATCC
Cac8I 193 GCCTGC GC[ACGT]^[ACGT]GC
843 GCTGGC
CfoI 206 GCGC GCG^C
831 GCGC
DraII 375 AGGTCCT [GA]G^G[ACGT]CC[CT]
899 AGGACCT
EcoO109I 375 AGGTCCT [GA]G^G[ACGT]CC[CT]
899 AGGACCT
HhaI 206 GCGC GCG^C
831 GCGC
HinP1I 206 GCGC G^CGC
831 GCGC
HphI 90 GGTGA GGTGA(8/7)
281 TCACC
MflI 71 GGATCC [GA]^GATC[CT]
101 GGATCC
MluNI 113 TGGCCA TGG^CCA
645 TGGCCA
MscI 113 TGGCCA TGG^CCA
645 TGGCCA
MslI 153 CATGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
249 CATAATGGTG
MvnI 39 CGCG CG^CG
205 CGCG
NciI 178 CCCGG CC^[GC]GG
734 CCCGG
NlaIII 153 CATG CATG^
768 CATG
PpuMI 375 AGGTCCT [GA]G^G[AT]CC[CT]
899 AGGACCT
PstI 172 CTGCAG CTGCA^G
460 CTGCAG
SfcI 172 CTGCAG C^T[GA][CT]AG
460 CTGCAG
Tsp509I 630 AATT ^AATT
925 AATT
XhoII 71 GGATCC [GA]^GATC[CT]
101 GGATCC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbsI BbvI BfaI BpuAI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstNI Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII ItaI MaeI MaeIII MboI
MboII MnlI MspI MvaI MwoI
NdeII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TseI TspRI