Restriction map for
pF1KE2259
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI AocI ApaI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbvCI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsmI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoT22I FseI
FspI HincII HindII HpaI MamI
MfeI MluI MroI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 867 GGTACC G^GTACC
AccI 157 GTCTAC GT^[AC][GT]AC
AcsI 396 GAATTC [GA]^AATT[CT]
Alw44I 1183 GTGCAC G^TGCAC
AlwNI 415 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 359 AGCGCT AGC^GCT
ApaLI 1183 GTGCAC G^TGCAC
ApoI 396 GAATTC [GA]^AATT[CT]
Asp718I 867 GGTACC G^GTACC
BanII 643 GAGCCC G[GA]GC[CT]^C
BbeI 1122 GGCGCC GGCGC^C
BbrPI 52 CACGTG CAC^GTG
BclI 902 TGATCA T^GATCA
BfaI 592 CTAG C^TAG
BglI 1172 GCCTGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 52 CACGTG [CT]AC^GT[GA]
Bsp1407I 1154 TGTACA T^GTACA
BspDI 454 ATCGAT AT^CGAT
BsrGI 1154 TGTACA T^GTACA
BssSI 832 CTCGTG CACGAG(-5/-1)
ClaI 454 ATCGAT AT^CGAT
EarI 1035 GAAGAG CTCTTC(1/4)
Eco47III 359 AGCGCT AGC^GCT
EcoRI 396 GAATTC G^AATTC
EcoRV 556 GATATC GAT^ATC
FbaI 902 TGATCA T^GATCA
HindIII 82 AAGCTT A^AGCTT
KasI 1122 GGCGCC G^GCGCC
KpnI 867 GGTACC GGTAC^C
Ksp632I 1035 GAAGAG CTCTTC(1/4)
KspI 1167 CCGCGG CCGC^GG
MaeI 592 CTAG C^TAG
MseI 214 TTAA T^TAA
NarI 1122 GGCGCC GG^CGCC
PflMI 221 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 52 CACGTG CAC^GTG
PmlI 52 CACGTG CAC^GTG
PshAI 990 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1167 CCGCGG CCGC^GG
ScaI 884 AGTACT AGT^ACT
SfoI 1122 GGCGCC GGC^GCC
SmaI 1042 CCCGGG CCC^GGG
SspBI 1154 TGTACA T^GTACA
TaqI 455 TCGA T^CGA
Tru9I 214 TTAA T^TAA
Tsp509I 397 AATT ^AATT
TthHB8I 455 TCGA T^CGA
Van91I 221 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 899 CCATGATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1042 CCCGGG C^CCGGG
XmaI 1042 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AflIII 53 ACGTGT A^C[GA][CT]GT
386 ACGTGT
AluI 83 AGCT AG^CT
95 AGCT
AvaI 1042 CCCGGG C^[CT]CG[GA]G
1279 CCCGAG
BanI 867 GGTACC G^G[CT][GA]CC
1122 GGCGCC
BpmI 425 CTCCAG CTGGAG(16/14)
1309 CTGGAG
BseRI 612 GAGGAG GAGGAG(10/8)
1312 GAGGAG
BsoBI 1042 CCCGGG C^[CT]CG[GA]G
1279 CCCGAG
BsrDI 409 CATTGC GCAATG(2/0)
1326 CATTGC
BstAPI 1226 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1254 GCACCTCCTGC
BstXI 1301 CCAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1472 CCAACTTCGTGG
BstYI 534 GGATCT [GA]^GATC[CT]
1304 AGATCC
DdeI 169 CTGAG C^T[ACGT]AG
839 CTGAG
EcoNI 116 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1308 CCTGGAGGAGG
EcoT14I 116 CCTTGG C^C[AT][AT]GG
530 CCTTGG
MflI 534 GGATCT [GA]^GATC[CT]
1304 AGATCC
StyI 116 CCTTGG C^C[AT][AT]GG
530 CCTTGG
XhoII 534 GGATCT [GA]^GATC[CT]
1304 AGATCC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AfaI AlwI
AspHI AvaII BalI BbsI BbvI
BcnI BmyI BpuAI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI TseI Tsp45I TspRI