Restriction map for
pF1KE2395
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AspEI
AspI AvaI AviII AvrII BamHI
BanII BbeI BbrPI BcgI BfrI
BglI BlnI BlpI Bpu1102I BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstUI CelII Cfr10I ClaI
CpoI DraI DraII DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoRV EcoT22I FauI FseI
FspI HaeII HgaI Hin1I HpaI
KasI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SspBI StuI SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 1906 GGTACC G^GTACC
AccI 867 GTATAC GT^[AC][GT]AC
AocI 436 CCTAAGG CC^T[ACGT]AGG
Asp700I 247 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1906 GGTACC G^GTACC
BalI 520 TGGCCA TGG^CCA
BanI 1906 GGTACC G^G[CT][GA]CC
BbvCI 2593 GCTGAGG CCTCAGC(-5/-2)
BciVI 2036 GTATCC GTATCC(6/5)
BclI 2016 TGATCA T^GATCA
BglII 1470 AGATCT A^GATCT
BpmI 2323 CTGGAG CTGGAG(16/14)
BsaI 713 GGTCTC GGTCTC(1/5)
BsmFI 42 GGGAC GGGAC(10/14)
BspHI 1088 TCATGA T^CATGA
BspLU11I 505 ACATGT A^CATGT
BspMI 739 GCAGGT ACCTGC(4/8)
Bst1107I 867 GTATAC GTA^TAC
BstBI 1940 TTCGAA TT^CGAA
BstEII 1846 GGTGACC G^GT[ACGT]ACC
BstPI 1846 GGTGACC G^GT[ACGT]ACC
BstXI 1394 CCATAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 867 GTATAC GTA^TAC
Bsu36I 436 CCTAAGG CC^T[ACGT]AGG
CfoI 2308 GCGC GCG^C
DrdI 1112 GACAGCGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 520 TGGCCA [CT]^GGCC[GA]
Eco81I 436 CCTAAGG CC^T[ACGT]AGG
EcoNI 2071 CCTTGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1846 GGTGACC G^GT[ACGT]ACC
FbaI 2016 TGATCA T^GATCA
HhaI 2308 GCGC GCG^C
HinP1I 2308 GCGC G^CGC
HincII 3171 GTCAAC GT[CT]^[GA]AC
HindII 3171 GTCAAC GT[CT]^[GA]AC
KpnI 1906 GGTACC GGTAC^C
MluNI 520 TGGCCA TGG^CCA
MscI 520 TGGCCA TGG^CCA
MspA1I 1898 CAGCTG C[AC]G^C[GT]G
NdeI 1611 CATATG CA^TATG
NspV 1940 TTCGAA TT^CGAA
PflMI 586 CCAAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1898 CAGCTG CAG^CTG
RcaI 1088 TCATGA T^CATGA
SexAI 709 ACCTGGT A^CC[AT]GGT
SfuI 1940 TTCGAA TT^CGAA
SpeI 1645 ACTAGT A^CTAGT
Van91I 586 CCAAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 897 TCTAGA T^CTAGA
XcmI 782 CCAAAGACTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 247 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 1640 CCGC CCGC(-3/-1)
877 GCGG
AflIII 505 ACATGT A^C[GA][CT]GT
3252 ACGTGT
AspHI 1996 GAGCAC G[AT]GC[AT]^C
2253 GAGCAC
AvaII 1267 GGACC G^G[AT]CC
1711 GGACC
BcnI 1283 CCCGG CC^[GC]GG
3059 CCGGG
BsaAI 1918 TACGTG [CT]AC^GT[GA]
1968 TACGTG
BsaBI 133 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1780 GATGGGGATC
BsiHKAI 1996 GAGCAC G[AT]GC[AT]^C
2253 GAGCAC
Cfr13I 1267 GGACC G^G[ACGT]CC
1711 GGACC
EcoRI 2332 GAATTC G^AATTC
2781 GAATTC
HapII 1284 CCGG C^CGG
3059 CCGG
HindIII 1528 AAGCTT A^AGCTT
2134 AAGCTT
HpaII 1284 CCGG C^CGG
3059 CCGG
MamI 133 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1780 GATGGGGATC
MspI 1284 CCGG C^CGG
3059 CCGG
NciI 1283 CCCGG CC^[GC]GG
3059 CCGGG
NspI 505 ACATGT [GA]CATG^[CT]
1498 GCATGT
Sau96I 1267 GGACC G^G[ACGT]CC
1711 GGACC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AseI AsnI BbsI BbvI BfaI
BmyI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI PshBI PstI
RsaI Sau3AI ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII