Restriction map for
pF1KE2351
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflIII AgeI Alw44I AlwI AlwNI
AocI ApaI ApaLI AscI Asp718I
AspHI AspI AviII AvrII BalI
BamHI BanII BbrPI BbsI BbvCI
BcgI BciVI BclI BlnI BpmI
BpuAI BsaAI BsaBI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraII DraIII EaeI EagI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT14I EcoT22I FauI FbaI
FseI FspI HgaI HincII HindII
HpaI HphI KpnI MaeII MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccII 7 CGCG CG^CG
AcyI 37 GGCGCC G[GA]^CG[CT]C
AfaI 137 GTAC GT^AC
AflII 901 CTTAAG C^TTAAG
AhdI 508 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 557 AGCGCT AGC^GCT
AseI 772 ATTAAT AT^TAAT
AsnI 772 ATTAAT AT^TAAT
AspEI 508 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 57 CTCGGG C^[CT]CG[GA]G
BanI 37 GGCGCC G^G[CT][GA]CC
BbeI 37 GGCGCC GGCGC^C
BcnI 81 CCCGG CC^[GC]GG
BfrI 901 CTTAAG C^TTAAG
BglI 29 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1238 AGATCT A^GATCT
BlpI 51 GCTCAGC GC^T[ACGT]AGC
BmyI 1224 GGGCAC G[AGT]GC[ACT]^C
Bpu1102I 51 GCTCAGC GC^T[ACGT]AGC
BsaHI 37 GGCGCC G[GA]^CG[CT]C
BseRI 70 GAGGAG GAGGAG(10/8)
BsmI 736 GCATTC GAATGC(1/-1)
BsoBI 57 CTCGGG C^[CT]CG[GA]G
Bsp1286I 1224 GGGCAC G[AGT]GC[ACT]^C
BspHI 1044 TCATGA T^CATGA
BstUI 7 CGCG CG^CG
BstYI 1238 AGATCT [GA]^GATC[CT]
CelII 51 GCTCAGC GC^T[ACGT]AGC
DpnII 1239 GATC ^GATC
DrdI 292 GACTGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 508 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 557 AGCGCT AGC^GCT
EcoRV 794 GATATC GAT^ATC
HapII 82 CCGG C^CGG
Hin1I 37 GGCGCC G[GA]^CG[CT]C
HindIII 1204 AAGCTT A^AGCTT
HpaII 82 CCGG C^CGG
KasI 37 GGCGCC G^GCGCC
KspI 6 CCGCGG CCGC^GG
MboI 1239 GATC ^GATC
MflI 1238 AGATCT [GA]^GATC[CT]
MspI 82 CCGG C^CGG
MvnI 7 CGCG CG^CG
MwoI 29 GCCTCCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 37 GGCGCC GG^CGCC
NciI 81 CCCGG CC^[GC]GG
NdeII 1239 GATC ^GATC
NspI 762 ACATGC [GA]CATG^[CT]
PflMI 1273 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1212 GACTC GAGTC(4/5)
PshBI 772 ATTAAT AT^TAAT
RcaI 1044 TCATGA T^CATGA
RsaI 137 GTAC GT^AC
SacII 6 CCGCGG CCGC^GG
Sau3AI 1239 GATC ^GATC
SexAI 811 ACCTGGT A^CC[AT]GGT
SfaNI 583 GATGC GCATC(5/9)
SfoI 37 GGCGCC GGC^GCC
SmlI 901 CTTAAG C^T[CT][GA]AG
Tsp45I 212 GTGAC ^GT[GC]AC
Van91I 1273 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1238 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BbvI 75 GCAGC GCAGC(8/12)
48 GCTGC
BfaI 709 CTAG C^TAG
1286 CTAG
BsaI 808 GAGACC GGTCTC(1/5)
715 GGTCTC
BstXI 111 CCAGGTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
580 CCAGATGCTTGG
Cac8I 346 GCGAGC GC[ACGT]^[ACGT]GC
766 GCAAGC
DraI 651 TTTAAA TTT^AAA
1183 TTTAAA
DsaI 6 CCGCGG C^C[GA][CT]GG
63 CCACGG
EarI 243 GAAGAG CTCTTC(1/4)
685 GAAGAG
EcoRI 1080 GAATTC G^AATTC
1294 GAATTC
Ksp632I 243 GAAGAG CTCTTC(1/4)
685 GAAGAG
MaeI 709 CTAG C^TAG
1286 CTAG
MspA1I 6 CCGCGG C[AC]G^C[GT]G
89 CAGCGG
NlaIV 37 GGCGCC GG[ACGT]^[ACGT]CC
576 GGGACC
SfcI 174 CTACAG C^T[GA][CT]AG
741 CTACAG
SspI 1150 AATATT AAT^ATT
1396 AATATT
TaqI 965 TCGA T^CGA
1134 TCGA
TseI 48 GCTGC G^C[AT]GC
75 GCAGC
TthHB8I 965 TCGA T^CGA
1134 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AcsI AluI ApoI Asp700I
AvaII BsaJI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
CfoI Cfr13I DdeI EcoRII Fnu4HI
FokI HaeII HaeIII HhaI HinP1I
HinfI ItaI MaeIII MboII MnlI
MseI MslI MvaI NlaIII Sau96I
ScrFI TfiI Tru9I Tsp509I TspRI
XmnI