Restriction map for
pF1KE2119
##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AflII AgeI AscI AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BfrI BglII BlnI BsaAI
BsaI BsiWI Bsp1407I BspDI BsrBI
BsrGI BssHII Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DrdI EcoNI
EcoO65I EcoRI EcoT22I FseI FspI
HpaI KasI KspI MluI NaeI
NarI NdeI NgoMIV NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacII SapI SbfI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 2233 GGTACC G^GTACC
AccI 253 GTCGAC GT^[AC][GT]AC
AccIII 2216 TCCGGA T^CCGGA
AflIII 2294 ACATGT A^C[GA][CT]GT
AhdI 2293 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 935 GTGCAC G^TGCAC
ApaLI 935 GTGCAC G^TGCAC
AseI 976 ATTAAT AT^TAAT
AsnI 976 ATTAAT AT^TAAT
Asp718I 2233 GGTACC G^GTACC
AspEI 2293 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 97 CCCGGG C^[CT]CG[GA]G
BalI 1583 TGGCCA TGG^CCA
BbvCI 2144 CCTCAGC CCTCAGC(-5/-2)
BciVI 2397 GTATCC GTATCC(6/5)
BlpI 990 GCTTAGC GC^T[ACGT]AGC
BpmI 467 CTCCAG CTGGAG(16/14)
Bpu1102I 990 GCTTAGC GC^T[ACGT]AGC
BsaBI 1599 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2216 TCCGGA T^CCGGA
BseRI 1641 CTCCTC GAGGAG(10/8)
BsmBI 1948 GAGACG CGTCTC(1/5)
BsoBI 97 CCCGGG C^[CT]CG[GA]G
BspEI 2216 TCCGGA T^CCGGA
BspLU11I 2294 ACATGT A^CATGT
BsrFI 180 GCCGGT [GA]^CCGG[CT]
BstBI 1474 TTCGAA TT^CGAA
BstXI 2404 CCAAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 990 GCTTAGC GC^T[ACGT]AGC
Cfr10I 180 GCCGGT [GA]^CCGG[CT]
DraII 2258 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 843 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2293 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 2258 AGGTCCT [GA]G^G[ACGT]CC[CT]
HincII 253 GTCGAC GT[CT]^[GA]AC
HindII 253 GTCGAC GT[CT]^[GA]AC
HindIII 865 AAGCTT A^AGCTT
KpnI 2233 GGTACC GGTAC^C
MamI 1599 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 582 CAATTG C^AATTG
MluNI 1583 TGGCCA TGG^CCA
MroI 2216 TCCGGA T^CCGGA
MscI 1583 TGGCCA TGG^CCA
MunI 582 CAATTG C^AATTG
NcoI 1986 CCATGG C^CATGG
NspV 1474 TTCGAA TT^CGAA
PflMI 1586 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2258 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 976 ATTAAT AT^TAAT
SacI 464 GAGCTC GAGCT^C
SalI 253 GTCGAC G^TCGAC
SexAI 2530 ACCTGGT A^CC[AT]GGT
SfuI 1474 TTCGAA TT^CGAA
SmaI 97 CCCGGG CCC^GGG
SmlI 2499 CTTGAG C^T[CT][GA]AG
SphI 1611 GCATGC GCATG^C
Van91I 1586 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2289 TCTAGA T^CTAGA
XmaCI 97 CCCGGG C^CCGGG
XmaI 97 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AclI 616 AACGTT AA^CGTT
929 AACGTT
AcyI 1171 GACGCC G[GA]^CG[CT]C
1950 GACGCC
AlwNI 1004 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
2626 CAGGGGCTG
Aor51HI 2477 AGCGCT AGC^GCT
2570 AGCGCT
ApaI 94 GGGCCC GGGCC^C
100 GGGCCC
Asp700I 418 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2208 GAAGTCTTTC
BanI 2084 GGCACC G^G[CT][GA]CC
2233 GGTACC
BbsI 418 GAAGAC GAAGAC(2/6)
2359 GAAGAC
BclI 480 TGATCA T^GATCA
2651 TGATCA
BglI 61 GCCGCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2327 GCCTAAGCGGC
BpuAI 418 GAAGAC GAAGAC(2/6)
2359 GAAGAC
BsaHI 1171 GACGCC G[GA]^CG[CT]C
1950 GACGCC
BsaWI 12 TCCGGT [AT]^CCGG[AT]
2216 TCCGGA
BsgI 1228 GTGCAG GTGCAG(16/14)
1574 GTGCAG
BsmI 2382 GAATGC GAATGC(1/-1)
2554 GAATGC
BspMI 1379 GCAGGT ACCTGC(4/8)
2027 GCAGGT
BsrDI 1087 GCAATG GCAATG(2/0)
1111 GCAATG
BssSI 1165 CACGAG CACGAG(-5/-1)
2071 CTCGTG
BstYI 299 AGATCC [GA]^GATC[CT]
1149 AGATCC
DraI 568 TTTAAA TTT^AAA
2095 TTTAAA
EagI 22 CGGCCG C^GGCCG
68 CGGCCG
EclXI 22 CGGCCG C^GGCCG
68 CGGCCG
Eco47III 2477 AGCGCT AGC^GCT
2570 AGCGCT
Eco52I 22 CGGCCG C^GGCCG
68 CGGCCG
EcoRV 364 GATATC GAT^ATC
1675 GATATC
FauI 103 CCCGC CCCGC(4/6)
2239 CCCGC
FbaI 480 TGATCA T^GATCA
2651 TGATCA
HgaI 1171 GACGC GACGC(5/10)
1950 GACGC
Hin1I 1171 GACGCC G[GA]^CG[CT]C
1950 GACGCC
MflI 299 AGATCC [GA]^GATC[CT]
1149 AGATCC
NheI 2139 GCTAGC G^CTAGC
2665 GCTAGC
NotI 21 GCGGCCGC GC^GGCCGC
67 GCGGCCGC
Psp1406I 616 AACGTT AA^CGTT
929 AACGTT
ScaI 854 AGTACT AGT^ACT
1761 AGTACT
XcmI 1125 CCAGAACGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2401 CCACCAAATCCCTGG
XhoII 299 AGATCC [GA]^GATC[CT]
1149 AGATCC
XmnI 418 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2208 GAAGTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApoI AspHI AvaII
BanII BbvI BcnI BfaI BmyI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI Eco81I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I