Restriction map for
pF1KE2495
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# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AclI AgeI AhdI Alw44I
ApaLI AscI Asp718I AspEI AvrII
BbeI BbrPI BciVI BlnI BsaAI
BsiEI BsiWI BspDI BspHI BssHII
Bst1107I BstBI BstZ17I ClaI DraI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FseI HpaI KasI
KpnI MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI SalI SfoI SfuI SgrAI
SnaBI SpeI SspI SwaI XhoI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AatII 3694 GACGTC GACGT^C
AccI 1048 GTCTAC GT^[AC][GT]AC
AccIII 710 TCCGGA T^CCGGA
AcsI 739 AAATTC [GA]^AATT[CT]
AflII 3244 CTTAAG C^TTAAG
AflIII 1478 ACATGT A^C[GA][CT]GT
ApaI 4685 GGGCCC GGGCC^C
ApoI 739 AAATTC [GA]^AATT[CT]
AseI 3265 ATTAAT AT^TAAT
AsnI 3265 ATTAAT AT^TAAT
BfrI 3244 CTTAAG C^TTAAG
BglII 1400 AGATCT A^GATCT
BsaBI 4878 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 710 TCCGGA [AT]^CCGG[AT]
BseAI 710 TCCGGA T^CCGGA
BsmI 5154 GCATTC GAATGC(1/-1)
Bsp1407I 398 TGTACA T^GTACA
BspEI 710 TCCGGA T^CCGGA
BspLU11I 1478 ACATGT A^CATGT
BsrGI 398 TGTACA T^GTACA
BstXI 4901 CCAACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 4985 CGGTCCG CG^G[AT]CCG
MamI 4878 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 710 TCCGGA T^CCGGA
NcoI 1583 CCATGG C^CATGG
PshBI 3265 ATTAAT AT^TAAT
RsrII 4985 CGGTCCG CG^G[AT]CCG
SbfI 1551 CCTGCAGG CCTGCA^GG
Sse8387I 1551 CCTGCAGG CCTGCA^GG
SspBI 398 TGTACA T^GTACA
XbaI 69 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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Asp700I 1302 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2124 GAAGGGCTTC
AspI 1941 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3049 GACAAGGTC
BbvCI 4434 CCTCAGC CCTCAGC(-5/-2)
2707 GCTGAGG
BclI 2259 TGATCA T^GATCA
2729 TGATCA
BstEII 604 GGTGACC G^GT[ACGT]ACC
909 GGTCACC
BstPI 604 GGTGACC G^GT[ACGT]ACC
909 GGTCACC
DraIII 102 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2008 CACTTTGTG
DrdI 4978 GACGATGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5088 GACAGAGCGGTC
EcoO65I 604 GGTGACC G^GT[ACGT]ACC
909 GGTCACC
FbaI 2259 TGATCA T^GATCA
2729 TGATCA
HindIII 3241 AAGCTT A^AGCTT
5761 AAGCTT
KspI 296 CCGCGG CCGC^GG
3572 CCGCGG
NaeI 1750 GCCGGC GCC^GGC
3463 GCCGGC
NgoMIV 1750 GCCGGC G^CCGGC
3463 GCCGGC
PflFI 1941 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3049 GACAAGGTC
PflMI 3933 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4776 CCAGCGGGTGG
SacII 296 CCGCGG CCGC^GG
3572 CCGCGG
ScaI 578 AGTACT AGT^ACT
1364 AGTACT
SexAI 221 ACCAGGT A^CC[AT]GGT
5603 ACCTGGT
SfiI 3462 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5268 GGCCAAGAAGGCC
SphI 2366 GCATGC GCATG^C
2910 GCATGC
Tth111I 1941 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3049 GACAAGGTC
Van91I 3933 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4776 CCAGCGGGTGG
XmnI 1302 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2124 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI AspHI
AvaI AvaII AviII BalI BamHI
BanI BanII BbsI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI