Restriction map for
pF1KE2601
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BalI
BamHI BanI BbeI BbsI BcgI
BfrI BglI BglII BlnI BpuAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV FseI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Alw44I 2128 GTGCAC G^TGCAC
AlwNI 67 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 404 GGGCCC GGGCC^C
ApaLI 2128 GTGCAC G^TGCAC
AspI 1102 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1853 CTCGAG C^[CT]CG[GA]G
AviII 686 TGCGCA TGC^GCA
BbrPI 1782 CACGTG CAC^GTG
BciVI 1521 GTATCC GTATCC(6/5)
BclI 650 TGATCA T^GATCA
BcnI 1999 CCCGG CC^[GC]GG
BlpI 78 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 78 GCTGAGC GC^T[ACGT]AGC
BsaAI 1782 CACGTG [CT]AC^GT[GA]
BsaI 709 GAGACC GGTCTC(1/5)
BseRI 212 GAGGAG GAGGAG(10/8)
BsoBI 1853 CTCGAG C^[CT]CG[GA]G
BspHI 647 TCATGA T^CATGA
BsrDI 690 CATTGC GCAATG(2/0)
BssSI 2170 CTCGTG CACGAG(-5/-1)
BstAPI 69 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 458 CCAGATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2163 GGATCT [GA]^GATC[CT]
Cac8I 683 GCATGC GC[ACGT]^[ACGT]GC
CelII 78 GCTGAGC GC^T[ACGT]AGC
EcoNI 1104 CCTAGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 518 CCATGG C^C[AT][AT]GG
EcoT22I 1086 ATGCAT ATGCA^T
FauI 4 GCGGG CCCGC(4/6)
FbaI 650 TGATCA T^GATCA
FspI 686 TGCGCA TGC^GCA
HaeII 87 GGCGCT [GA]GCGC^[CT]
HgaI 2035 GACGC GACGC(5/10)
MaeII 1783 ACGT A^CGT
MflI 2163 GGATCT [GA]^GATC[CT]
NciI 1999 CCCGG CC^[GC]GG
NcoI 518 CCATGG C^CATGG
NdeI 1361 CATATG CA^TATG
NsiI 1086 ATGCAT ATGCA^T
PaeR7I 1853 CTCGAG C^TCGAG
PflFI 1102 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1782 CACGTG CAC^GTG
PmlI 1782 CACGTG CAC^GTG
PpuMI 1208 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 647 TCATGA T^CATGA
SacI 1678 GAGCTC GAGCT^C
SapI 938 GAAGAGC GCTCTTC(1/4)
SexAI 1278 ACCTGGT A^CC[AT]GGT
SfcI 959 CTGTAG C^T[GA][CT]AG
SphI 683 GCATGC GCATG^C
StuI 656 AGGCCT AGG^CCT
StyI 518 CCATGG C^C[AT][AT]GG
TfiI 431 GATTC G^A[AT]TC
Tth111I 1102 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1853 CTCGAG C^TCGAG
XhoII 2163 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccII 84 CGCG CG^CG
2037 CGCG
BanII 404 GGGCCC G[GA]GC[CT]^C
1678 GAGCTC
BbvCI 659 CCTCAGC CCTCAGC(-5/-2)
1204 GCTGAGG
BsaBI 728 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1516 GATGTGTATC
BsmBI 2016 CGTCTC CGTCTC(1/5)
2033 GAGACG
Bsp1407I 414 TGTACA T^GTACA
1898 TGTACA
BspMI 664 GCAGGT ACCTGC(4/8)
1861 GCAGGT
BsrGI 414 TGTACA T^GTACA
1898 TGTACA
BstUI 84 CGCG CG^CG
2037 CGCG
DraII 403 AGGGCCC [GA]G^G[ACGT]CC[CT]
1208 AGGACCC
DsaI 518 CCATGG C^C[GA][CT]GG
1001 CCGTGG
EaeI 58 TGGCCG [CT]^GGCC[GA]
2012 TGGCCG
EcoO109I 403 AGGGCCC [GA]G^G[ACGT]CC[CT]
1208 AGGACCC
HapII 41 CCGG C^CGG
2000 CCGG
HpaII 41 CCGG C^CGG
2000 CCGG
MamI 728 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1516 GATGTGTATC
MspI 41 CCGG C^CGG
2000 CCGG
MvnI 84 CGCG CG^CG
2037 CGCG
PvuII 70 CAGCTG CAG^CTG
2099 CAGCTG
SmlI 737 CTCAAG C^T[CT][GA]AG
1853 CTCGAG
SspBI 414 TGTACA T^GTACA
1898 TGTACA
SspI 983 AATATT AAT^ATT
1441 AATATT
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI CfoI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I