Restriction map for
pF1KE2336
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AviII AvrII BanI BbeI BbrPI
BfrI BglI BlnI BlpI Bpu1102I
BsaHI BseAI BsiEI BsmBI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
BssSI CelII Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoT22I FseI
FspI Hin1I HindIII KasI KpnI
KspI MfeI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SwaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 3871 GTATAC GT^[AC][GT]AC
AflIII 60 ACGCGT A^C[GA][CT]GT
AseI 349 ATTAAT AT^TAAT
AsnI 349 ATTAAT AT^TAAT
AspI 2826 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 2728 GGGCTC G[GA]GC[CT]^C
BbvCI 1109 CCTCAGC CCTCAGC(-5/-2)
BcgI 983 GCAAAATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3498 GGATAC GTATCC(6/5)
BsaAI 1015 TACGTG [CT]AC^GT[GA]
BsiWI 401 CGTACG C^GTACG
BsmI 3441 GAATGC GAATGC(1/-1)
Bsp1407I 3146 TGTACA T^GTACA
BspMI 2931 GCAGGT ACCTGC(4/8)
BsrBI 153 CCGCTC CCGCTC(-3/-3)
BsrGI 3146 TGTACA T^GTACA
Bst1107I 3871 GTATAC GTA^TAC
BstBI 1720 TTCGAA TT^CGAA
BstEII 1092 GGTTACC G^GT[ACGT]ACC
BstPI 1092 GGTTACC G^GT[ACGT]ACC
BstZ17I 3871 GTATAC GTA^TAC
DraII 2896 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 2896 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1092 GGTTACC G^GT[ACGT]ACC
EcoRV 1182 GATATC GAT^ATC
HaeII 2669 AGCGCC [GA]GCGC^[CT]
HincII 2371 GTTAAC GT[CT]^[GA]AC
HindII 2371 GTTAAC GT[CT]^[GA]AC
HpaI 2371 GTTAAC GTT^AAC
MluI 60 ACGCGT A^CGCGT
NdeI 514 CATATG CA^TATG
NspI 1230 GCATGT [GA]CATG^[CT]
NspV 1720 TTCGAA TT^CGAA
PflFI 2826 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3892 CCACATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2896 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 349 ATTAAT AT^TAAT
SfuI 1720 TTCGAA TT^CGAA
SspBI 3146 TGTACA T^GTACA
SspI 1474 AATATT AAT^ATT
Tth111I 2826 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3892 CCACATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1993 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 945 CCTCAGG CC^T[ACGT]AGG
3361 CCTCAGG
AvaI 1375 CTCGGG C^[CT]CG[GA]G
1455 CTCGGG
BalI 99 TGGCCA TGG^CCA
551 TGGCCA
BclI 615 TGATCA T^GATCA
1034 TGATCA
BfaI 1994 CTAG C^TAG
2275 CTAG
BsaBI 1564 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2440 GATAGAAATC
BsaWI 2424 TCCGGT [AT]^CCGG[AT]
3229 ACCGGA
BsoBI 1375 CTCGGG C^[CT]CG[GA]G
1455 CTCGGG
BsrDI 2537 CATTGC GCAATG(2/0)
3690 CATTGC
BstAPI 670 GCAGTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1690 GCAGTAGATGC
BstXI 329 CCATATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3094 CCACCAGCCTGG
Bsu36I 945 CCTCAGG CC^T[ACGT]AGG
3361 CCTCAGG
CfoI 1191 GCGC GCG^C
2670 GCGC
Eco81I 945 CCTCAGG CC^T[ACGT]AGG
3361 CCTCAGG
EcoNI 165 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
941 CCTGCCTCAGG
FauI 33 CCCGC CCCGC(4/6)
2834 CCCGC
FbaI 615 TGATCA T^GATCA
1034 TGATCA
HhaI 1191 GCGC GCG^C
2670 GCGC
HinP1I 1191 GCGC G^CGC
2670 GCGC
MaeI 1994 CTAG C^TAG
2275 CTAG
MamI 1564 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2440 GATAGAAATC
MluNI 99 TGGCCA TGG^CCA
551 TGGCCA
MscI 99 TGGCCA TGG^CCA
551 TGGCCA
NcoI 374 CCATGG C^CATGG
554 CCATGG
PvuII 1201 CAGCTG CAG^CTG
1552 CAGCTG
StuI 1287 AGGCCT AGG^CCT
2096 AGGCCT
XcmI 374 CCATGGATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3091 CCACCACCAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BamHI BbsI BbvI BcnI
BglII BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI