Restriction map for
pF1KE1803
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII AccI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI Asp700I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BpuAI BsaAI BsaHI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HpaI HphI
KasI Ksp632I KspI MfeI MluI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 560 GGTACC G^GTACC
AccII 7 CGCG CG^CG
AccIII 900 TCCGGA T^CCGGA
AclI 826 AACGTT AA^CGTT
ApaI 81 GGGCCC GGGCC^C
Asp718I 560 GGTACC G^GTACC
BalI 461 TGGCCA TGG^CCA
BanI 560 GGTACC G^G[CT][GA]CC
BbvI 185 GCAGC GCAGC(8/12)
BcnI 64 CCCGG CC^[GC]GG
BfaI 691 CTAG C^TAG
BlpI 484 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 484 GCTCAGC GC^T[ACGT]AGC
BsaBI 898 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 900 TCCGGA [AT]^CCGG[AT]
BseAI 900 TCCGGA T^CCGGA
BsgI 88 CTGCAC GTGCAG(16/14)
BspEI 900 TCCGGA T^CCGGA
BspHI 672 TCATGA T^CATGA
BspMI 32 ACCTGC ACCTGC(4/8)
BsrBI 135 CCGCTC CCGCTC(-3/-3)
BsrDI 605 GCAATG GCAATG(2/0)
BsrI 894 ACTGG ACTGG(1/-1)
BssSI 76 CACGAG CACGAG(-5/-1)
BstAPI 248 GCACTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 7 CGCG CG^CG
BstXI 140 CCATGTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 484 GCTCAGC GC^T[ACGT]AGC
DdeI 485 CTCAG C^T[ACGT]AG
DraI 931 TTTAAA TTT^AAA
EaeI 461 TGGCCA [CT]^GGCC[GA]
HgaI 188 GCGTC GACGC(5/10)
HindIII 475 AAGCTT A^AGCTT
KpnI 560 GGTACC GGTAC^C
MaeI 691 CTAG C^TAG
MamI 898 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 461 TGGCCA TGG^CCA
MroI 900 TCCGGA T^CCGGA
MscI 461 TGGCCA TGG^CCA
MvnI 7 CGCG CG^CG
NciI 64 CCCGG CC^[GC]GG
PflMI 782 CCAAGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 826 AACGTT AA^CGTT
PstI 183 CTGCAG CTGCA^G
RcaI 672 TCATGA T^CATGA
StuI 362 AGGCCT AGG^CCT
TfiI 340 GATTC G^A[AT]TC
TseI 185 GCAGC G^C[AT]GC
Tsp45I 724 GTCAC ^GT[GC]AC
TspRI 253 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 782 CCAAGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AseI 330 ATTAAT AT^TAAT
508 ATTAAT
AsnI 330 ATTAAT AT^TAAT
508 ATTAAT
BamHI 222 GGATCC G^GATCC
897 GGATCC
BanII 68 GAGCCC G[GA]GC[CT]^C
81 GGGCCC
BglII 456 AGATCT A^GATCT
584 AGATCT
BmyI 68 GAGCCC G[AGT]GC[ACT]^C
81 GGGCCC
BpmI 310 CTGGAG CTGGAG(16/14)
854 CTGGAG
BsaI 686 GAGACC GGTCTC(1/5)
876 GGTCTC
BsaJI 63 CCCCGG C^C[ACGT][ACGT]GG
156 CCTGGG
BseRI 46 GAGGAG GAGGAG(10/8)
468 GAGGAG
Bsp1286I 68 GAGCCC G[AGT]GC[ACT]^C
81 GGGCCC
BstNI 156 CCTGG CC^[AT]GG
174 CCAGG
DraII 80 AGGGCCC [GA]G^G[ACGT]CC[CT]
81 GGGCCCC
EcoO109I 80 AGGGCCC [GA]G^G[ACGT]CC[CT]
81 GGGCCCC
EcoRI 798 GAATTC G^AATTC
805 GAATTC
EcoRII 156 CCTGG ^CC[AT]GG
174 CCAGG
HapII 65 CCGG C^CGG
901 CCGG
HpaII 65 CCGG C^CGG
901 CCGG
MaeII 722 ACGT A^CGT
827 ACGT
MaeIII 724 GTCAC ^GT[ACGT]AC
846 GTTAC
MslI 141 CATGTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
280 CATGAAAATG
MspI 65 CCGG C^CGG
901 CCGG
MvaI 156 CCTGG CC^[AT]GG
174 CCAGG
PshBI 330 ATTAAT AT^TAAT
508 ATTAAT
SfcI 183 CTGCAG C^T[GA][CT]AG
695 CTACAG
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BsiYI BslI BsmAI BssKI
BstF5I BstYI Cac8I CfoI Cfr13I
DpnII FauI Fnu4HI FokI HaeIII
HhaI HinP1I HinfI ItaI MboI
MboII MflI MnlI MseI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
Tru9I Tsp509I TthHB8I XhoII