Restriction map for
pF1KE0900
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# NO CUT ENZYMES ( 76 enzymes) #
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AatII Acc65I AccIII Aor51HI ApaI
AscI Asp718I AspI AvaI AviII
AvrII BbeI BciVI BcnI BglI
BglII BlnI BlpI Bpu1102I BseAI
BsiEI BsiWI BsmBI BsoBI BspEI
BsrBI BssHII BstBI BstEII BstPI
BstXI CelII CpoI DraII EagI
EclXI Eco47III Eco52I EcoO109I EcoO65I
FseI FspI HaeII KasI KpnI
KspI MfeI MroI MunI NaeI
NarI NciI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PmeI
PpuMI PshAI PvuI RsrII SacI
SacII SalI SfiI SfoI SfuI
SgrAI SmaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 7504 CGCG CG^CG
AclI 2488 AACGTT AA^CGTT
AcyI 8969 GGCGTC G[GA]^CG[CT]C
AflII 4845 CTTAAG C^TTAAG
AgeI 9114 ACCGGT A^CCGGT
Alw44I 8663 GTGCAC G^TGCAC
AocI 7915 CCTAAGG CC^T[ACGT]AGG
ApaLI 8663 GTGCAC G^TGCAC
BbrPI 8660 CACGTG CAC^GTG
BbvCI 556 GCTGAGG CCTCAGC(-5/-2)
BcgI 10023 CGAAGAACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4845 CTTAAG C^TTAAG
BsaHI 8969 GGCGTC G[GA]^CG[CT]C
BsaWI 9114 ACCGGT [AT]^CCGG[AT]
BspDI 3513 ATCGAT AT^CGAT
BsrFI 9114 ACCGGT [GA]^CCGG[CT]
BssSI 6320 CTCGTG CACGAG(-5/-1)
BstAPI 9543 GCATATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 7504 CGCG CG^CG
Bsu36I 7915 CCTAAGG CC^T[ACGT]AGG
CfoI 8090 GCGC GCG^C
Cfr10I 9114 ACCGGT [GA]^CCGG[CT]
ClaI 3513 ATCGAT AT^CGAT
Eco81I 7915 CCTAAGG CC^T[ACGT]AGG
EcoRV 5282 GATATC GAT^ATC
FauI 4583 GCGGG CCCGC(4/6)
HhaI 8090 GCGC GCG^C
Hin1I 8969 GGCGTC G[GA]^CG[CT]C
HinP1I 8090 GCGC G^CGC
MluI 7503 ACGCGT A^CGCGT
MvnI 7504 CGCG CG^CG
NheI 4579 GCTAGC G^CTAGC
PinAI 9114 ACCGGT A^CCGGT
PmaCI 8660 CACGTG CAC^GTG
PmlI 8660 CACGTG CAC^GTG
Psp1406I 2488 AACGTT AA^CGTT
SbfI 2133 CCTGCAGG CCTGCA^GG
SexAI 7053 ACCTGGT A^CC[AT]GGT
SphI 9552 GCATGC GCATG^C
Sse8387I 2133 CCTGCAGG CCTGCA^GG
SwaI 6387 ATTTAAAT ATTT^AAAT
XcmI 5799 CCAAAATATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AhdI 2895 GACTCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8235 GACAGTTGGTC
AseI 3193 ATTAAT AT^TAAT
7345 ATTAAT
AsnI 3193 ATTAAT AT^TAAT
7345 ATTAAT
AspEI 2895 GACTCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8235 GACAGTTGGTC
BanI 4830 GGTGCC G^G[CT][GA]CC
9913 GGCACC
BsaI 4741 GAGACC GGTCTC(1/5)
1184 GGTCTC
Bst1107I 7620 GTATAC GTA^TAC
8364 GTATAC
BstZ17I 7620 GTATAC GTA^TAC
8364 GTATAC
DraIII 5837 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
7410 CACAAAGTG
Eam1105I 2895 GACTCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8235 GACAGTTGGTC
EcoNI 9324 CCTTAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
9625 CCTGGTACAGG
HapII 9115 CCGG C^CGG
9874 CCGG
HgaI 7505 GCGTC GACGC(5/10)
8970 GCGTC
HpaI 4224 GTTAAC GTT^AAC
8997 GTTAAC
HpaII 9115 CCGG C^CGG
9874 CCGG
MspI 9115 CCGG C^CGG
9874 CCGG
PflMI 3511 CCATCGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
8139 CCAAAAAGTGG
PshBI 3193 ATTAAT AT^TAAT
7345 ATTAAT
ScaI 5164 AGTACT AGT^ACT
8344 AGTACT
SnaBI 6098 TACGTA TAC^GTA
7623 TACGTA
StuI 4712 AGGCCT AGG^CCT
9399 AGGCCT
Van91I 3511 CCATCGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
8139 CCAAAAAGTGG
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# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BamHI BanII
BbsI BbvI BclI BfaI BmyI
BpmI BpuAI BsaAI BsaBI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BspLU11I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DrdI DsaI EaeI EarI EcoRI
EcoRII EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NcoI
NdeI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI