Restriction map for
pF1KB5924
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AlwI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BsaAI BsaHI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspMI BsrGI BssSI BstBI
BstEII BstPI BstXI ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HindIII
KasI KpnI KspI MaeII MluI
MluNI MroI MscI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 13 CGCG CG^CG
AhdI 529 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 529 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 553 GAGCTC G[AT]GC[AT]^C
AvaI 1121 CCCGAG C^[CT]CG[GA]G
BbsI 811 GAAGAC GAAGAC(2/6)
BcnI 30 CCGGG CC^[GC]GG
BglII 581 AGATCT A^GATCT
BlpI 399 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 399 GCTCAGC GC^T[ACGT]AGC
BpuAI 811 GAAGAC GAAGAC(2/6)
BsaBI 745 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1540 GAGACC GGTCTC(1/5)
BsaWI 458 ACCGGA [AT]^CCGG[AT]
BsiHKAI 553 GAGCTC G[AT]GC[AT]^C
BsoBI 1121 CCCGAG C^[CT]CG[GA]G
BspHI 1235 TCATGA T^CATGA
BsrBI 55 GAGCGG CCGCTC(-3/-3)
BsrFI 34 GCCGGC [GA]^CCGG[CT]
BssHII 12 GCGCGC G^CGCGC
Bst1107I 997 GTATAC GTA^TAC
BstAPI 267 GCAGATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 13 CGCG CG^CG
BstYI 581 AGATCT [GA]^GATC[CT]
BstZ17I 997 GTATAC GTA^TAC
CelII 399 GCTCAGC GC^T[ACGT]AGC
Cfr10I 34 GCCGGC [GA]^CCGG[CT]
DraI 151 TTTAAA TTT^AAA
DrdI 1249 GACCTTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 992 CCATGG C^C[GA][CT]GG
Eam1105I 529 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 159 CCTTATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1276 GAATTC G^AATTC
FauI 57 GCGGG CCCGC(4/6)
HpaI 897 GTTAAC GTT^AAC
HphI 658 TCACC GGTGA(8/7)
MamI 745 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 541 CAATTG C^AATTG
MflI 581 AGATCT [GA]^GATC[CT]
MunI 541 CAATTG C^AATTG
MvnI 13 CGCG CG^CG
NaeI 34 GCCGGC GCC^GGC
NciI 30 CCGGG CC^[GC]GG
NcoI 992 CCATGG C^CATGG
NgoMIV 34 GCCGGC G^CCGGC
PpuMI 59 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1343 CTGCAG CTGCA^G
RcaI 1235 TCATGA T^CATGA
SacI 553 GAGCTC GAGCT^C
SfaNI 602 GCATC GCATC(5/9)
XbaI 933 TCTAGA T^CTAGA
XcmI 1361 CCAGGGGCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 581 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 715 GTAGAC GT^[AC][GT]AC
997 GTATAC
AflIII 181 ACATGT A^C[GA][CT]GT
1242 ACATGT
AlwNI 268 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
1418 CAGACACTG
AocI 63 CCTGAGG CC^T[ACGT]AGG
661 CCTCAGG
BanII 553 GAGCTC G[GA]GC[CT]^C
1124 GAGCCC
BfaI 283 CTAG C^TAG
934 CTAG
BglI 679 GCCATTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
916 GCCCAAGAGGC
BmyI 553 GAGCTC G[AGT]GC[ACT]^C
1124 GAGCCC
BsmI 1338 GAATGC GAATGC(1/-1)
212 GCATTC
Bsp1286I 553 GAGCTC G[AGT]GC[ACT]^C
1124 GAGCCC
BspLU11I 181 ACATGT A^CATGT
1242 ACATGT
BsrDI 836 GCAATG GCAATG(2/0)
857 GCAATG
Bsu36I 63 CCTGAGG CC^T[ACGT]AGG
661 CCTCAGG
DpnII 582 GATC ^GATC
1156 GATC
EaeI 629 CGGCCA [CT]^GGCC[GA]
1358 CGGCCA
Eco81I 63 CCTGAGG CC^T[ACGT]AGG
661 CCTCAGG
EcoT14I 992 CCATGG C^C[AT][AT]GG
1251 CCTTGG
MaeI 283 CTAG C^TAG
934 CTAG
MboI 582 GATC ^GATC
1156 GATC
MslI 350 CATTAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
832 CACAGCAATG
NdeII 582 GATC ^GATC
1156 GATC
NlaIV 59 GGGTCC GG[ACGT]^[ACGT]CC
1364 GGGGCC
NspI 181 ACATGT [GA]CATG^[CT]
1242 ACATGT
PvuII 770 CAGCTG CAG^CTG
959 CAGCTG
SapI 823 GAAGAGC GCTCTTC(1/4)
501 GCTCTTC
Sau3AI 582 GATC ^GATC
1156 GATC
ScaI 204 AGTACT AGT^ACT
1523 AGTACT
SfcI 1074 CTATAG C^T[GA][CT]AG
1343 CTGCAG
StyI 992 CCATGG C^C[AT][AT]GG
1251 CCTTGG
TspRI 1326 CACTG CA[GC]TG[ACGT][ACGT]^
1422 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII ItaI Ksp632I MaeIII MboII
MnlI MseI MspA1I MspI MvaI
MwoI NlaIII PleI RsaI Sau96I
ScrFI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TthHB8I