##################################
# NO CUT ENZYMES (  217 enzymes) #
##################################

AatII       Acc65I      AccIII      AciI        AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BbvI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BlnI        BlpI        BmyI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        Fnu4HI      FokI        FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       ItaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvaI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TseI        
Tsp45I      Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccI          250  GTCTAC               GT^[AC][GT]AC
AccII         294  CGCG                 CG^CG
AcsI          130  AAATTT               [GA]^AATT[CT]
ApoI          130  AAATTT               [GA]^AATT[CT]
BbsI          208  GTCTTC               GAAGAC(2/6)
BcgI           36  CGAGAAATCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         310  AGATCT               A^GATCT
BpmI          239  CTGGAG               CTGGAG(16/14)
BpuAI         208  GTCTTC               GAAGAC(2/6)
BsrDI         232  GCAATG               GCAATG(2/0)
BstUI         294  CGCG                 CG^CG
BstXI         107  CCACCCACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         310  AGATCT               [GA]^GATC[CT]
DdeI          404  CTAAG                C^T[ACGT]AG
HinfI         465  GATTC                G^A[ACGT]TC
MaeIII        386  GTTAC                ^GT[ACGT]AC
MflI          310  AGATCT               [GA]^GATC[CT]
MnlI           96  GAGG                 CCTC(7/6)
MseI          494  TTAA                 T^TAA
MvnI          294  CGCG                 CG^CG
PflMI         356  CCAAAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
TaqI           35  TCGA                 T^CGA
TfiI          465  GATTC                G^A[AT]TC
Tru9I         494  TTAA                 T^TAA
TspRI         189  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        35  TCGA                 T^CGA
Van91I        356  CCAAAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          104  CCACCACCCACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         310  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BsmFI         193  GGGAC                GGGAC(10/14)
              182  GTCCC               
BsrI          362  ACTGG                ACTGG(1/-1)
              389  ACTGG               
DpnII         225  GATC                 ^GATC
              311  GATC                
HphI          462  GGTGA                GGTGA(8/7)
              486  GGTGA               
MboI          225  GATC                 ^GATC
              311  GATC                
MboII          14  TCTTC                GAAGA(8/7)
              209  TCTTC               
NdeII         225  GATC                 ^GATC
              311  GATC                
Sau3AI        225  GATC                 ^GATC
              311  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

BsiYI       BslI        NlaIII      Tsp509I