Restriction map for
pF1KE1747
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TfiI Tru9I
Tth111I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 373 GACGTC GACGT^C
AccI 376 GTCTAC GT^[AC][GT]AC
AcyI 373 GACGTC G[GA]^CG[CT]C
AocI 15 CCTCAGG CC^T[ACGT]AGG
AspHI 625 GAGCTC G[AT]GC[AT]^C
AvaII 561 GGACC G^G[AT]CC
BclI 221 TGATCA T^GATCA
BglI 719 GCCCGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 99 CTCCAG CTGGAG(16/14)
BsaHI 373 GACGTC G[GA]^CG[CT]C
BseRI 386 CTCCTC GAGGAG(10/8)
BsgI 39 GTGCAG GTGCAG(16/14)
BsiEI 687 CGGTCG CG[GA][CT]^CG
BsiHKAI 625 GAGCTC G[AT]GC[AT]^C
Bsp1407I 125 TGTACA T^GTACA
BsrBI 556 GAGCGG CCGCTC(-3/-3)
BsrGI 125 TGTACA T^GTACA
BstAPI 41 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 15 CCTCAGG CC^T[ACGT]AGG
DsaI 520 CCACGG C^C[GA][CT]GG
EarI 423 GAAGAG CTCTTC(1/4)
Eco81I 15 CCTCAGG CC^T[ACGT]AGG
FbaI 221 TGATCA T^GATCA
Hin1I 373 GACGTC G[GA]^CG[CT]C
HinfI 550 GACTC G^A[ACGT]TC
HphI 249 GGTGA GGTGA(8/7)
Ksp632I 423 GAAGAG CTCTTC(1/4)
MaeIII 662 GTGAC ^GT[ACGT]AC
MspA1I 102 CAGCTG C[AC]G^C[GT]G
PflMI 351 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 550 GACTC GAGTC(4/5)
PpuMI 560 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 102 CAGCTG CAG^CTG
SacI 625 GAGCTC GAGCT^C
SfaNI 235 GATGC GCATC(5/9)
SmaI 584 CCCGGG CCC^GGG
SspBI 125 TGTACA T^GTACA
Tsp45I 662 GTGAC ^GT[GC]AC
Tsp509I 498 AATT ^AATT
Van91I 351 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 584 CCCGGG C^CCGGG
XmaI 584 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaI 584 CCCGGG C^[CT]CG[GA]G
630 CCCGAG
BalI 174 TGGCCA TGG^CCA
656 TGGCCA
BsmFI 560 GGGAC GGGAC(10/14)
474 GTCCC
BsoBI 584 CCCGGG C^[CT]CG[GA]G
630 CCCGAG
BspMI 155 ACCTGC ACCTGC(4/8)
338 GCAGGT
BsrI 465 CCAGT ACTGG(1/-1)
659 CCAGT
BstXI 165 CCACAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
177 CCATGTGGATGG
BstYI 161 AGATCC [GA]^GATC[CT]
488 AGATCC
DraII 7 GGGGCCC [GA]G^G[ACGT]CC[CT]
560 GGGACCC
EaeI 174 TGGCCA [CT]^GGCC[GA]
656 TGGCCA
EcoO109I 7 GGGGCCC [GA]G^G[ACGT]CC[CT]
560 GGGACCC
FauI 11 CCCGC CCCGC(4/6)
558 GCGGG
HaeII 538 AGCGCC [GA]GCGC^[CT]
634 AGCGCC
MaeII 374 ACGT A^CGT
572 ACGT
MboII 272 GAAGA GAAGA(8/7)
423 GAAGA
MflI 161 AGATCC [GA]^GATC[CT]
488 AGATCC
MluNI 174 TGGCCA TGG^CCA
656 TGGCCA
MscI 174 TGGCCA TGG^CCA
656 TGGCCA
MslI 166 CACAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
178 CATGTGGATG
TaqI 323 TCGA T^CGA
690 TCGA
TthHB8I 323 TCGA T^CGA
690 TCGA
XhoII 161 AGATCC [GA]^GATC[CT]
488 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI MboI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfcI TseI TspRI