Restriction map for
pF1KE2129
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII Acc65I AccI AccIII AcyI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI AscI Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoT22I FseI FspI Hin1I
KasI KpnI KspI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 68 CGCG CG^CG
AclI 373 AACGTT AA^CGTT
AflIII 257 ACATGT A^C[GA][CT]GT
AhdI 1364 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 820 ATTAAT AT^TAAT
AsnI 820 ATTAAT AT^TAAT
AspEI 1364 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2514 GAGCTC G[AT]GC[AT]^C
BanI 2298 GGTGCC G^G[CT][GA]CC
BglII 811 AGATCT A^GATCT
BsaBI 2524 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1005 GTGCAG GTGCAG(16/14)
BsiEI 1074 CGGTCG CG[GA][CT]^CG
BsiHKAI 2514 GAGCTC G[AT]GC[AT]^C
BspLU11I 257 ACATGT A^CATGT
BsrFI 197 ACCGGC [GA]^CCGG[CT]
BstAPI 764 GCAATGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 68 CGCG CG^CG
BstXI 1352 CCATTTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 197 ACCGGC [GA]^CCGG[CT]
DraI 1095 TTTAAA TTT^AAA
DsaI 2762 CCATGG C^C[GA][CT]GG
Eam1105I 1364 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2014 GAAGAG CTCTTC(1/4)
EcoNI 714 CCTGCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1872 GATATC GAT^ATC
HaeII 49 GGCGCT [GA]GCGC^[CT]
HincII 376 GTTAAC GT[CT]^[GA]AC
HindII 376 GTTAAC GT[CT]^[GA]AC
HpaI 376 GTTAAC GTT^AAC
Ksp632I 2014 GAAGAG CTCTTC(1/4)
MamI 2524 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1799 CAATTG C^AATTG
MunI 1799 CAATTG C^AATTG
MvnI 68 CGCG CG^CG
NcoI 2762 CCATGG C^CATGG
PaeR7I 476 CTCGAG C^TCGAG
PflMI 2240 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1757 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 820 ATTAAT AT^TAAT
Psp1406I 373 AACGTT AA^CGTT
SacI 2514 GAGCTC GAGCT^C
SgrAI 196 CACCGGCG C[GA]^CCGG[CT]G
SmaI 44 CCCGGG CCC^GGG
SphI 1647 GCATGC GCATG^C
StuI 834 AGGCCT AGG^CCT
Tsp45I 658 GTGAC ^GT[GC]AC
Van91I 2240 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 425 CCAATGAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 476 CTCGAG C^TCGAG
XmaCI 44 CCCGGG C^CCGGG
XmaI 44 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 2284 GTAC GT^AC
2599 GTAC
ApaI 22 GGGCCC GGGCC^C
1548 GGGCCC
AvaI 44 CCCGGG C^[CT]CG[GA]G
476 CTCGAG
BclI 1068 TGATCA T^GATCA
2177 TGATCA
BseRI 292 GAGGAG GAGGAG(10/8)
2263 GAGGAG
BsmAI 1718 GAGAC GTCTC(1/5)
2678 GTCTC
BsoBI 44 CCCGGG C^[CT]CG[GA]G
476 CTCGAG
EcoT14I 1553 CCAAGG C^C[AT][AT]GG
2762 CCATGG
FauI 129 CCCGC CCCGC(4/6)
138 CCCGC
FbaI 1068 TGATCA T^GATCA
2177 TGATCA
HgaI 1675 GACGC GACGC(5/10)
2209 GACGC
HindIII 1854 AAGCTT A^AGCTT
2335 AAGCTT
MaeII 374 ACGT A^CGT
2114 ACGT
MslI 330 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1923 CATTATGGTG
PstI 1867 CTGCAG CTGCA^G
2602 CTGCAG
RsaI 2284 GTAC GT^AC
2599 GTAC
SspI 925 AATATT AAT^ATT
1559 AATATT
StyI 1553 CCAAGG C^C[AT][AT]GG
2762 CCATGG
TfiI 1179 GAATC G^A[AT]TC
1631 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I AvaII BalI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsiYI BslI BsmFI
BsmI Bsp1286I BspHI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RcaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII XmnI