Restriction map for
pF1KE1965
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BspLU11I BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
StuI SwaI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AhdI 226 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 667 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 226 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 270 GTGCTC G[AT]GC[AT]^C
AspI 442 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 877 CCCGAG C^[CT]CG[GA]G
BanI 1208 GGCACC G^G[CT][GA]CC
BcgI 372 CGATTTGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1468 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1329 AGATCT A^GATCT
BpmI 582 CTGGAG CTGGAG(16/14)
BsgI 333 CTGCAC GTGCAG(16/14)
BsiHKAI 270 GTGCTC G[AT]GC[AT]^C
BsmBI 342 GAGACG CGTCTC(1/5)
BsmI 100 GAATGC GAATGC(1/-1)
BsoBI 877 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1295 TGTACA T^GTACA
BspMI 262 ACCTGC ACCTGC(4/8)
BsrBI 117 GAGCGG CCGCTC(-3/-3)
BsrDI 866 GCAATG GCAATG(2/0)
BsrGI 1295 TGTACA T^GTACA
DrdI 520 GACCTCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 226 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 1177 GTTGAC GT[CT]^[GA]AC
HindII 1177 GTTGAC GT[CT]^[GA]AC
NcoI 992 CCATGG C^CATGG
NspI 1082 GCATGC [GA]CATG^[CT]
PflFI 442 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1048 CCACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 124 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 944 CAGCTG CAG^CTG
SphI 1082 GCATGC GCATG^C
SspBI 1295 TGTACA T^GTACA
SspI 511 AATATT AAT^ATT
TaqI 371 TCGA T^CGA
Tth111I 442 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 371 TCGA T^CGA
Van91I 1048 CCACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 397 AAATTT [GA]^AATT[CT]
955 GAATTT
ApoI 397 AAATTT [GA]^AATT[CT]
955 GAATTT
BcnI 228 CCGGG CC^[GC]GG
1263 CCGGG
BfaI 62 CTAG C^TAG
1043 CTAG
BmyI 270 GTGCTC G[AGT]GC[ACT]^C
380 GTGCCC
Bsp1286I 270 GTGCTC G[AGT]GC[ACT]^C
380 GTGCCC
BstXI 267 CCAGTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1325 CCAAAGATCTGG
DsaI 112 CCGTGG C^C[GA][CT]GG
992 CCATGG
EcoT22I 535 ATGCAT ATGCA^T
1169 ATGCAT
FauI 119 GCGGG CCCGC(4/6)
714 GCGGG
HapII 228 CCGG C^CGG
1263 CCGG
HpaII 228 CCGG C^CGG
1263 CCGG
MaeI 62 CTAG C^TAG
1043 CTAG
MaeIII 731 GTGAC ^GT[ACGT]AC
1502 GTCAC
MseI 500 TTAA T^TAA
1391 TTAA
MspA1I 47 CCGCTG C[AC]G^C[GT]G
944 CAGCTG
MspI 228 CCGG C^CGG
1263 CCGG
NciI 228 CCGGG CC^[GC]GG
1263 CCGGG
NsiI 535 ATGCAT ATGCA^T
1169 ATGCAT
SfcI 825 CTGTAG C^T[GA][CT]AG
1344 CTATAG
Tru9I 500 TTAA T^TAA
1391 TTAA
Tsp45I 731 GTGAC ^GT[GC]AC
1502 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BbvI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MboI MboII MflI MluNI MnlI
MscI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI TseI Tsp509I TspRI XcmI
XhoII