Restriction map for
pF1KE2555
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BciVI BclI BfaI
BfrI BglI BlnI BsaBI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MaeI MamI
MluI MroI MseI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AvaI 181 CCCGAG C^[CT]CG[GA]G
AviII 290 TGCGCA TGC^GCA
BamHI 155 GGATCC G^GATCC
BbrPI 1135 CACGTG CAC^GTG
BbvCI 489 CCTCAGC CCTCAGC(-5/-2)
BglII 866 AGATCT A^GATCT
BlpI 715 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 715 GCTGAGC GC^T[ACGT]AGC
BsaAI 1135 CACGTG [CT]AC^GT[GA]
BsgI 422 GTGCAG GTGCAG(16/14)
BsmI 293 GCATTC GAATGC(1/-1)
BsoBI 181 CCCGAG C^[CT]CG[GA]G
BspHI 1298 TCATGA T^CATGA
BspMI 1260 GCAGGT ACCTGC(4/8)
BssSI 1273 CACGAG CACGAG(-5/-1)
BstAPI 1260 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 942 GGTGACC G^GT[ACGT]ACC
BstPI 942 GGTGACC G^GT[ACGT]ACC
CelII 715 GCTGAGC GC^T[ACGT]AGC
DraIII 1239 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 213 GACGCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 942 GGTGACC G^GT[ACGT]ACC
FspI 290 TGCGCA TGC^GCA
KspI 19 CCGCGG CCGC^GG
MaeII 1136 ACGT A^CGT
MfeI 204 CAATTG C^AATTG
MunI 204 CAATTG C^AATTG
PflMI 498 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 972 GAGTC GAGTC(4/5)
PmaCI 1135 CACGTG CAC^GTG
PmlI 1135 CACGTG CAC^GTG
RcaI 1298 TCATGA T^CATGA
SacI 718 GAGCTC GAGCT^C
SacII 19 CCGCGG CCGC^GG
SapI 240 GCTCTTC GCTCTTC(1/4)
Tsp509I 205 AATT ^AATT
Van91I 498 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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Aor51HI 342 AGCGCT AGC^GCT
879 AGCGCT
Asp700I 87 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
836 GAAAACCTTC
AspHI 162 GAGCAC G[AT]GC[AT]^C
718 GAGCTC
BbeI 1251 GGCGCC GGCGC^C
1291 GGCGCC
BcgI 564 CGAGACCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
437 GCAGCCTCTTCG
BsaI 565 GAGACC GGTCTC(1/5)
706 GAGACC
BsiHKAI 162 GAGCAC G[AT]GC[AT]^C
718 GAGCTC
BsmBI 11 GAGACG CGTCTC(1/5)
283 GAGACG
Eco47III 342 AGCGCT AGC^GCT
879 AGCGCT
EcoNI 789 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1350 CCTCTTCAAGG
KasI 1251 GGCGCC G^GCGCC
1291 GGCGCC
NarI 1251 GGCGCC GG^CGCC
1291 GGCGCC
PpuMI 177 GGGTCCC [GA]G^G[AT]CC[CT]
1147 AGGTCCC
PvuII 226 CAGCTG CAG^CTG
451 CAGCTG
SexAI 35 ACCTGGT A^CC[AT]GGT
1226 ACCTGGT
SfcI 430 CTACAG C^T[GA][CT]AG
474 CTACAG
SfoI 1251 GGCGCC GGC^GCC
1291 GGCGCC
TfiI 744 GATTC G^A[AT]TC
805 GAATC
XcmI 498 CCAGGGCCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
929 CCATCACAGCCATGG
XmnI 87 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
836 GAAAACCTTC
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaII BalI BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI
XhoII