Restriction map for
pF1KB8478
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BbeI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BseAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FspI HindIII KasI MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tsp509I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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Acc65I 1769 GGTACC G^GTACC
AlwNI 343 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1769 GGTACC G^GTACC
BbrPI 1408 CACGTG CAC^GTG
BbsI 1546 GTCTTC GAAGAC(2/6)
BbvCI 600 CCTCAGC CCTCAGC(-5/-2)
BcgI 1478 GCATGCCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2150 GTATCC GTATCC(6/5)
BpuAI 1546 GTCTTC GAAGAC(2/6)
BsaAI 1408 CACGTG [CT]AC^GT[GA]
BsaWI 1532 ACCGGA [AT]^CCGG[AT]
BsmBI 236 GAGACG CGTCTC(1/5)
Bsp1407I 392 TGTACA T^GTACA
BsrGI 392 TGTACA T^GTACA
EcoNI 183 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 88 GGCCGGCC GGCCGG^CC
HpaI 1006 GTTAAC GTT^AAC
KpnI 1769 GGTACC GGTAC^C
MseI 1007 TTAA T^TAA
NaeI 89 GCCGGC GCC^GGC
NgoMIV 89 GCCGGC G^CCGGC
PmaCI 1408 CACGTG CAC^GTG
PmlI 1408 CACGTG CAC^GTG
SacI 608 GAGCTC GAGCT^C
SmaI 215 CCCGGG CCC^GGG
SspBI 392 TGTACA T^GTACA
Tru9I 1007 TTAA T^TAA
XmaCI 215 CCCGGG C^CCGGG
XmaI 215 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 910 GACGTC GACGT^C
1649 GACGTC
AfaI 393 GTAC GT^AC
1770 GTAC
ApaI 595 GGGCCC GGGCC^C
2469 GGGCCC
BalI 267 TGGCCA TGG^CCA
332 TGGCCA
BsaI 101 GGTCTC GGTCTC(1/5)
1213 GGTCTC
BsiEI 1402 CGACCG CG[GA][CT]^CG
2060 CGACCG
BsrDI 528 CATTGC GCAATG(2/0)
1667 GCAATG
BsrFI 89 GCCGGC [GA]^CCGG[CT]
589 GCCGGT
BssHII 1282 GCGCGC G^CGCGC
1453 GCGCGC
BstAPI 678 GCACTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
971 GCATTGACTGC
BstEII 193 GGTGACC G^GT[ACGT]ACC
1831 GGTAACC
BstPI 193 GGTGACC G^GT[ACGT]ACC
1831 GGTAACC
BstYI 1205 AGATCC [GA]^GATC[CT]
1801 GGATCT
Cfr10I 89 GCCGGC [GA]^CCGG[CT]
589 GCCGGT
EcoO65I 193 GGTGACC G^GT[ACGT]ACC
1831 GGTAACC
EcoT14I 1235 CCAAGG C^C[AT][AT]GG
1968 CCTTGG
HaeII 1131 GGCGCT [GA]GCGC^[CT]
1929 GGCGCT
HincII 646 GTCAAC GT[CT]^[GA]AC
1006 GTTAAC
HindII 646 GTCAAC GT[CT]^[GA]AC
1006 GTTAAC
MflI 1205 AGATCC [GA]^GATC[CT]
1801 GGATCT
MluNI 267 TGGCCA TGG^CCA
332 TGGCCA
MscI 267 TGGCCA TGG^CCA
332 TGGCCA
NspI 1478 GCATGC [GA]CATG^[CT]
1684 GCATGC
PflMI 636 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
961 CCATACCGTGG
RsaI 393 GTAC GT^AC
1770 GTAC
SapI 12 GAAGAGC GCTCTTC(1/4)
498 GCTCTTC
SphI 1478 GCATGC GCATG^C
1684 GCATGC
StyI 1235 CCAAGG C^C[AT][AT]GG
1968 CCTTGG
TfiI 1567 GATTC G^A[AT]TC
1777 GATTC
Van91I 636 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
961 CCATACCGTGG
XhoII 1205 AGATCC [GA]^GATC[CT]
1801 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AocI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI TaqI TseI Tsp45I
TspRI TthHB8I XcmI