################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI SalI SapI SbfI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AlwNI 463 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 145 AGCGCT AGC^GCT ApaI 532 GGGCCC GGGCC^C AspHI 963 GAGCTC G[AT]GC[AT]^C BalI 1456 TGGCCA TGG^CCA BamHI 741 GGATCC G^GATCC BbrPI 18 CACGTG CAC^GTG BbvCI 852 GCTGAGG CCTCAGC(-5/-2) BsaAI 18 CACGTG [CT]AC^GT[GA] BsaI 1150 GAGACC GGTCTC(1/5) BsgI 828 CTGCAC GTGCAG(16/14) BsiEI 1138 CGGTCG CG[GA][CT]^CG BsiHKAI 963 GAGCTC G[AT]GC[AT]^C BspMI 602 ACCTGC ACCTGC(4/8) BssSI 454 CACGAG CACGAG(-5/-1) BstEII 657 GGTCACC G^GT[ACGT]ACC BstPI 657 GGTCACC G^GT[ACGT]ACC BstXI 498 CCAGTTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 741 GGATCC [GA]^GATC[CT] CpoI 1384 CGGTCCG CG^G[AT]CCG DpnII 742 GATC ^GATC Eco47III 145 AGCGCT AGC^GCT EcoO65I 657 GGTCACC G^GT[ACGT]ACC EcoT14I 746 CCATGG C^C[AT][AT]GG KspI 174 CCGCGG CCGC^GG MboI 742 GATC ^GATC MflI 741 GGATCC [GA]^GATC[CT] MluNI 1456 TGGCCA TGG^CCA MscI 1456 TGGCCA TGG^CCA NaeI 33 GCCGGC GCC^GGC NcoI 746 CCATGG C^CATGG NdeII 742 GATC ^GATC NgoMIV 33 GCCGGC G^CCGGC NspI 1511 ACATGC [GA]CATG^[CT] PaeR7I 616 CTCGAG C^TCGAG PmaCI 18 CACGTG CAC^GTG PmlI 18 CACGTG CAC^GTG RsrII 1384 CGGTCCG CG^G[AT]CCG SacI 963 GAGCTC GAGCT^C SacII 174 CCGCGG CCGC^GG Sau3AI 742 GATC ^GATC ScaI 779 AGTACT AGT^ACT SgrAI 308 CGCCGGTG C[GA]^CCGG[CT]G SmlI 616 CTCGAG C^T[CT][GA]AG StyI 746 CCATGG C^C[AT][AT]GG TfiI 918 GAATC G^A[AT]TC XcmI 1532 CCAAACTCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 616 CTCGAG C^TCGAG XhoII 741 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AflIII 19 ACGTGT A^C[GA][CT]GT 1107 ACGTGT AlwI 742 GATCC GGATC(4/5) 741 GGATC Asp700I 1289 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1344 GAAACCATTC BbeI 130 GGCGCC GGCGC^C 306 GGCGCC BbsI 1398 GAAGAC GAAGAC(2/6) 1081 GTCTTC BglI 133 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 752 GCCAGCAGGGC BpuAI 1398 GAAGAC GAAGAC(2/6) 1081 GTCTTC BsmBI 38 CGTCTC CGTCTC(1/5) 154 CGTCTC BsmI 904 GAATGC GAATGC(1/-1) 1091 GCATTC BsrBI 54 CCGCTC CCGCTC(-3/-3) 288 GAGCGG DsaI 174 CCGCGG C^C[GA][CT]GG 746 CCATGG EcoT22I 906 ATGCAT ATGCA^T 1513 ATGCAT KasI 130 GGCGCC G^GCGCC 306 GGCGCC MboII 1398 GAAGA GAAGA(8/7) 1082 TCTTC MseI 943 TTAA T^TAA 1208 TTAA NarI 130 GGCGCC GG^CGCC 306 GGCGCC NsiI 906 ATGCAT ATGCA^T 1513 ATGCAT SfcI 73 CTACAG C^T[GA][CT]AG 864 CTACAG SfoI 130 GGCGCC GGC^GCC 306 GGCGCC SmaI 105 CCCGGG CCC^GGG 330 CCCGGG TaqI 617 TCGA T^CGA 665 TCGA Tru9I 943 TTAA T^TAA 1208 TTAA Tsp509I 877 AATT ^AATT 1493 AATT TthHB8I 617 TCGA T^CGA 665 TCGA XmaCI 105 CCCGGG C^CCGGG 330 CCCGGG XmaI 105 CCCGGG C^CCGGG 330 CCCGGG XmnI 1289 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1344 GAAACCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PpuMI PvuII RsaI Sau96I ScrFI SfaNI TseI Tsp45I TspRI