Restriction map for
pF1KE2487
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspHI AviII AvrII
BbeI BbrPI BcgI BciVI BfrI
BglII BlnI BsaAI BsaBI BsaHI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoT22I FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MseI MslI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI TfiI Tru9I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 23 GGTACC G^GTACC
AccI 1513 GTCTAC GT^[AC][GT]AC
AccIII 461 TCCGGA T^CCGGA
AcsI 439 GAATTT [GA]^AATT[CT]
AhdI 931 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 439 GAATTT [GA]^AATT[CT]
Asp718I 23 GGTACC G^GTACC
AspEI 931 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1707 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1943 TGGCCA TGG^CCA
BamHI 203 GGATCC G^GATCC
BbvCI 772 GCTGAGG CCTCAGC(-5/-2)
BglI 1080 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1007 GAGACC GGTCTC(1/5)
BsaWI 461 TCCGGA [AT]^CCGG[AT]
BseAI 461 TCCGGA T^CCGGA
BspEI 461 TCCGGA T^CCGGA
BsrDI 1340 GCAATG GCAATG(2/0)
BssSI 1570 CACGAG CACGAG(-5/-1)
BstEII 1782 GGTAACC G^GT[ACGT]ACC
BstPI 1782 GGTAACC G^GT[ACGT]ACC
BstXI 678 CCAGGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 725 GCGC GCG^C
DraIII 1314 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1943 TGGCCA [CT]^GGCC[GA]
Eam1105I 931 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 543 CCTTACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1782 GGTAACC G^GT[ACGT]ACC
EcoRV 587 GATATC GAT^ATC
HaeII 724 AGCGCC [GA]GCGC^[CT]
HgaI 1711 GCGTC GACGC(5/10)
HhaI 725 GCGC GCG^C
HinP1I 725 GCGC G^CGC
KpnI 23 GGTACC GGTAC^C
MluNI 1943 TGGCCA TGG^CCA
MroI 461 TCCGGA T^CCGGA
MscI 1943 TGGCCA TGG^CCA
NcoI 1464 CCATGG C^CATGG
NspI 1506 ACATGC [GA]CATG^[CT]
PflFI 1707 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 135 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 1764 CCTGCAGG CCTGCA^GG
SmaI 1355 CCCGGG CCC^GGG
Sse8387I 1764 CCTGCAGG CCTGCA^GG
StuI 651 AGGCCT AGG^CCT
Tsp509I 440 AATT ^AATT
Tth111I 1707 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 135 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 732 CCACAATCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1355 CCCGGG C^CCGGG
XmaI 1355 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AvaI 851 CCCGAG C^[CT]CG[GA]G
1355 CCCGGG
BanI 23 GGTACC G^G[CT][GA]CC
432 GGCACC
BanII 243 GAGCCC G[GA]GC[CT]^C
636 GGGCTC
BlpI 423 GCTTAGC GC^T[ACGT]AGC
1724 GCTCAGC
BpmI 1027 CTGGAG CTGGAG(16/14)
1173 CTGGAG
Bpu1102I 423 GCTTAGC GC^T[ACGT]AGC
1724 GCTCAGC
BsmFI 1209 GGGAC GGGAC(10/14)
1022 GTCCC
BsoBI 851 CCCGAG C^[CT]CG[GA]G
1355 CCCGGG
BspHI 1454 TCATGA T^CATGA
1559 TCATGA
BstYI 203 GGATCC [GA]^GATC[CT]
467 GGATCT
CelII 423 GCTTAGC GC^T[ACGT]AGC
1724 GCTCAGC
DsaI 824 CCACGG C^C[GA][CT]GG
1464 CCATGG
EarI 304 CTCTTC CTCTTC(1/4)
1956 CTCTTC
Ksp632I 304 CTCTTC CTCTTC(1/4)
1956 CTCTTC
MaeII 114 ACGT A^CGT
1517 ACGT
MflI 203 GGATCC [GA]^GATC[CT]
467 GGATCT
PpuMI 5 GGGTCCT [GA]G^G[AT]CC[CT]
1436 AGGACCT
PshAI 1525 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1659 GACACTGGTC
RcaI 1454 TCATGA T^CATGA
1559 TCATGA
XhoII 203 GGATCC [GA]^GATC[CT]
467 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbsI BbvI BclI BcnI BfaI
BmyI BpuAI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I