Restriction map for
pF1KB6853
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiWI BsiYI
BslI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRII EcoRV EcoT22I FauI
FokI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MroI MslI
MspI MunI MvaI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI ScrFI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Asp700I 379 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 161 TGGCCA TGG^CCA
BamHI 81 GGATCC G^GATCC
BclI 519 TGATCA T^GATCA
BpmI 169 CTGGAG CTGGAG(16/14)
BseRI 229 GAGGAG GAGGAG(10/8)
BsiEI 441 CGATCG CG[GA][CT]^CG
BsmAI 147 GAGAC GTCTC(1/5)
BsrDI 260 CATTGC GCAATG(2/0)
BsrI 76 CCAGT ACTGG(1/-1)
BstYI 81 GGATCC [GA]^GATC[CT]
Cac8I 255 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 32 GGGCC G^G[ACGT]CC
DraII 31 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 134 CCATGG C^C[GA][CT]GG
EaeI 161 TGGCCA [CT]^GGCC[GA]
EcoO109I 31 GGGGCCT [GA]G^G[ACGT]CC[CT]
FbaI 519 TGATCA T^GATCA
MaeIII 130 GTGAC ^GT[ACGT]AC
MflI 81 GGATCC [GA]^GATC[CT]
MluNI 161 TGGCCA TGG^CCA
MscI 161 TGGCCA TGG^CCA
MspA1I 253 CAGCTG C[AC]G^C[GT]G
NcoI 134 CCATGG C^CATGG
NlaIII 135 CATG CATG^
PvuI 441 CGATCG CGAT^CG
PvuII 253 CAGCTG CAG^CTG
Sau96I 32 GGGCC G^G[ACGT]CC
SfcI 313 CTACAG C^T[GA][CT]AG
SspI 92 AATATT AAT^ATT
Tsp45I 130 GTGAC ^GT[GC]AC
TspRI 77 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 81 GGATCC [GA]^GATC[CT]
XmnI 379 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 82 GATCC GGATC(4/5)
81 GGATC
BanII 232 GAGCTC G[GA]GC[CT]^C
320 GAGCTC
BbvI 246 GCAGC GCAGC(8/12)
264 GCAGC
BsaJI 27 CCTTGG C^C[ACGT][ACGT]GG
134 CCATGG
DdeI 145 CTGAG C^T[ACGT]AG
414 CTCAG
EcoT14I 27 CCTTGG C^C[AT][AT]GG
134 CCATGG
Fnu4HI 246 GCAGC GC^[ACGT]GC
264 GCAGC
HaeIII 33 GGCC GG^CC
162 GGCC
HinfI 4 GAGTC G^A[ACGT]TC
412 GACTC
ItaI 246 GCAGC GC^[ACGT]GC
264 GCAGC
MseI 40 TTAA T^TAA
511 TTAA
NlaIV 31 GGGGCC GG[ACGT]^[ACGT]CC
81 GGATCC
PleI 412 GACTC GAGTC(4/5)
4 GAGTC
SacI 232 GAGCTC GAGCT^C
320 GAGCTC
StyI 27 CCTTGG C^C[AT][AT]GG
134 CCATGG
Tru9I 40 TTAA T^TAA
511 TTAA
TseI 246 GCAGC G^C[AT]GC
264 GCAGC
Tsp509I 185 AATT ^AATT
332 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI AspHI BfaI BmyI BsiHKAI
Bsp1286I DpnII MaeI MboI MboII
MnlI MwoI NdeII Sau3AI SmlI