Restriction map for
pF1KB4865
##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
Asp700I AspEI AspI AviII AvrII
BamHI BbvCI BcgI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaI
BsiWI BsmBI Bsp1407I BspDI BspMI
BsrGI BssHII BssSI BstAPI BstBI
Bsu36I CelII ClaI CpoI DraI
DrdI Eam1105I EarI Eco81I EcoRI
EcoRV EcoT22I FseI FspI HgaI
HpaI Ksp632I KspI MfeI MluI
MunI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 110 GGTACC G^GTACC
AccIII 180 TCCGGA T^CCGGA
AcyI 1027 GGCGCC G[GA]^CG[CT]C
AflIII 2180 ACATGT A^C[GA][CT]GT
Aor51HI 911 AGCGCT AGC^GCT
AseI 2398 ATTAAT AT^TAAT
AsnI 2398 ATTAAT AT^TAAT
Asp718I 110 GGTACC G^GTACC
BbeI 1027 GGCGCC GGCGC^C
BbrPI 2002 CACGTG CAC^GTG
BsaBI 710 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1027 GGCGCC G[GA]^CG[CT]C
BsaWI 180 TCCGGA [AT]^CCGG[AT]
BseAI 180 TCCGGA T^CCGGA
BspEI 180 TCCGGA T^CCGGA
BspHI 1532 TCATGA T^CATGA
BspLU11I 2180 ACATGT A^CATGT
BsrBI 16 CCGCTC CCGCTC(-3/-3)
BsrDI 2543 GCAATG GCAATG(2/0)
Bst1107I 1261 GTATAC GTA^TAC
BstXI 1871 CCACTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1688 GGATCT [GA]^GATC[CT]
BstZ17I 1261 GTATAC GTA^TAC
EagI 53 CGGCCG C^GGCCG
EclXI 53 CGGCCG C^GGCCG
Eco47III 911 AGCGCT AGC^GCT
Eco52I 53 CGGCCG C^GGCCG
EcoNI 1464 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 1027 GGCGCC G[GA]^CG[CT]C
HincII 2025 GTTGAC GT[CT]^[GA]AC
HindII 2025 GTTGAC GT[CT]^[GA]AC
HindIII 570 AAGCTT A^AGCTT
KasI 1027 GGCGCC G^GCGCC
KpnI 110 GGTACC GGTAC^C
MamI 710 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1688 GGATCT [GA]^GATC[CT]
MroI 180 TCCGGA T^CCGGA
NaeI 55 GCCGGC GCC^GGC
NarI 1027 GGCGCC GG^CGCC
NcoI 731 CCATGG C^CATGG
NdeI 1934 CATATG CA^TATG
NgoMIV 55 GCCGGC G^CCGGC
NspI 2180 ACATGT [GA]CATG^[CT]
PflMI 1930 CCAACATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2002 CACGTG CAC^GTG
PmlI 2002 CACGTG CAC^GTG
PshBI 2398 ATTAAT AT^TAAT
PstI 905 CTGCAG CTGCA^G
RcaI 1532 TCATGA T^CATGA
ScaI 2752 AGTACT AGT^ACT
SexAI 696 ACCAGGT A^CC[AT]GGT
SfoI 1027 GGCGCC GGC^GCC
SmaI 106 CCCGGG CCC^GGG
SmlI 2011 CTCAAG C^T[CT][GA]AG
SspI 1055 AATATT AAT^ATT
Van91I 1930 CCAACATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1688 GGATCT [GA]^GATC[CT]
XmaCI 106 CCCGGG C^CCGGG
XmaI 106 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 982 GTAGAC GT^[AC][GT]AC
1261 GTATAC
AccII 753 CGCG CG^CG
1676 CGCG
AlwNI 237 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
1362 CAGAAACTG
AspHI 31 GTGCTC G[AT]GC[AT]^C
2484 GAGCAC
AvaI 106 CCCGGG C^[CT]CG[GA]G
1696 CCCGAG
BalI 1880 TGGCCA TGG^CCA
2261 TGGCCA
BciVI 974 GGATAC GTATCC(6/5)
2494 GGATAC
BclI 203 TGATCA T^GATCA
1583 TGATCA
BfaI 461 CTAG C^TAG
631 CTAG
BsaAI 256 TACGTG [CT]AC^GT[GA]
2002 CACGTG
BsgI 392 GTGCAG GTGCAG(16/14)
2005 GTGCAG
BsiEI 53 CGGCCG CG[GA][CT]^CG
624 CGACCG
BsiHKAI 31 GTGCTC G[AT]GC[AT]^C
2484 GAGCAC
BsmI 514 GAATGC GAATGC(1/-1)
722 GCATTC
BsoBI 106 CCCGGG C^[CT]CG[GA]G
1696 CCCGAG
BsrFI 55 GCCGGC [GA]^CCGG[CT]
626 ACCGGC
BstEII 1392 GGTAACC G^GT[ACGT]ACC
2278 GGTGACC
BstPI 1392 GGTAACC G^GT[ACGT]ACC
2278 GGTGACC
BstUI 753 CGCG CG^CG
1676 CGCG
Cfr10I 55 GCCGGC [GA]^CCGG[CT]
626 ACCGGC
DraIII 386 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
458 CACCTAGTG
DsaI 731 CCATGG C^C[GA][CT]GG
1895 CCACGG
EcoO65I 1392 GGTAACC G^GT[ACGT]ACC
2278 GGTGACC
FauI 917 CCCGC CCCGC(4/6)
1423 CCCGC
FbaI 203 TGATCA T^GATCA
1583 TGATCA
MaeI 461 CTAG C^TAG
631 CTAG
MluNI 1880 TGGCCA TGG^CCA
2261 TGGCCA
MscI 1880 TGGCCA TGG^CCA
2261 TGGCCA
MspA1I 1766 CAGCTG C[AC]G^C[GT]G
2248 CAGCTG
MvnI 753 CGCG CG^CG
1676 CGCG
PvuII 1766 CAGCTG CAG^CTG
2248 CAGCTG
SfaNI 1371 GATGC GCATC(5/9)
1490 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI